Cell culture and Tissue culture
Arefeh Kamali; Abbas Zare MirakAbadi
Volume 2, Issue 2 , October 2021, , Pages 21-27
Abstract
Introduction and Aim: Many colubrid snakes produce toxic oral secretions and some species have caused severe reactions in humans. In this research toxicity of Duvernoy´s gland secretion (DGS) from Hemorrhois ravergieri (aglyph) has been studied.Methods: In this study, the effect of Hemorrhois ravergieri ...
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Introduction and Aim: Many colubrid snakes produce toxic oral secretions and some species have caused severe reactions in humans. In this research toxicity of Duvernoy´s gland secretion (DGS) from Hemorrhois ravergieri (aglyph) has been studied.Methods: In this study, the effect of Hemorrhois ravergieri saliva on human dermal fibroblasts (HDF) cells growth was determined by the inverted microscope and MTT assay. The integrity of the cell membrane through LDH release was evaluated as well.Results: The MTT assay and Neutral red assay showed a significant (p˂0.05) cytotoxic effect of Hemorrhois ravergieri salvia on HDF cells growth after 24 h treatment. Additionally, Hemorrhois ravergieri venom caused a significant increase in LDH release (p˂0.05). Numerous morphological abnormalities were observed in cells exposed to the DGS and showed loss of their common polygonal shape, appearing as several roughly rounded cells of variable size. Conclusion: The Hemorrhois ravergieri saliva causes cytotoxic effects on HDF cells by the necrotic mechanism. This colubrid snake bite causes local symptoms, so this research suggests a more careful evaluation of the victims when considering the medical treatment to be adopted.
Toxicology
Abbas Zare MirakAbadi; Parvin Horrieh
Volume 1, Issue 1 , September 2020, , Pages 1-8
Abstract
Introduction and Aim: Echis carinatus are small in size and are considered to be very biting invasive snakes. Snake venom is a type of snake saliva that is secreted through the salivary glands and stored in the venom sac. A problem in human societies, especially in rural areas, is snake bites, which ...
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Introduction and Aim: Echis carinatus are small in size and are considered to be very biting invasive snakes. Snake venom is a type of snake saliva that is secreted through the salivary glands and stored in the venom sac. A problem in human societies, especially in rural areas, is snake bites, which are not treated properly. The aim of this study is evaluation of toxicity of saliva of Echis carinatus sochureki on cells growth and the mechanisms involved. Methods: In this study, the effect of Echis carinatus sochureki saliva on human dermal fibroblasts (HDF) cells growth was determined by the inverted microscope, MTT assay, and Neutral red assay. The integrity of the cell membrane through LDH release was also measured. Results: The MTT assay and Neutral red assay showed a significant (p < /em>˂0.001) cytotoxic effect of Echis carinatus sochureki salvia on HDF cells growth after 24 hours treatment. Also, Echis carinatus sochureki venom caused a significant (p < /em>˂0.001) increase in LDH release. Various morphological abnormalities were observed in cells. Conclusion: The Echis carinatus sochureki saliva causes cytotoxic effects on HDF cells by the necrotic mechanism. The results of this study will be useful for future research in this field, as well as therapeutic methods for snake bites.
