tissue engineering and Regenerative medicine
Sona Zare; Rahim Ahmadi; DIBA SAMADI
Abstract
Background and Aim: Despite numerous studies on the biological properties and differentiability of umbilical cord-derived mesenchymal stem cells, these studies are still ongoing in order to achieve new findings. Therefore, the present study investigates the biological properties of umbilical cord-derived ...
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Background and Aim: Despite numerous studies on the biological properties and differentiability of umbilical cord-derived mesenchymal stem cells, these studies are still ongoing in order to achieve new findings. Therefore, the present study investigates the biological properties of umbilical cord-derived mesenchymal stem cells and their ability to differentiate into osteocyte and adipocyte.Materials and Methods: In this experimental laboratory study, 30 whole placenta specimens were prepared from the mothers under cesarean section and kept under standardized conditions. The mesenchymal cells were isolated by enzymatic method and their morphological characteristics were examined by microscopy and absorption spectroscopy and their biological properties, in particular expression of CD markers, were determined by flow cytometry. Finally, mesenchymal stem cells were cultured in specific media in order to differentiate into osteocyte and adipocyte. Data were analyzed using descriptive statistics.Results: Morphological and physical examinations by microscope and absorption spectroscopy as well as presenting of CD44, CD73, CD90, and CD105 markers and lacking CD34 and CD45 markers demonstrated the mesenchymal entity of stem cells. Mesenchymal stem cells successfully differentiated into osteocyte and adipocyte.Conclusion: Human cord-derived mesenchymal stem cells can differentiate into adult fat and bone cells. In this respect, the use of cord-derived mesenchymal cells could be of significant interest in cell therapy.
Cell culture and Tissue culture
Shahin Seyf; Rahim Ahmadi; Sara Najafi
Abstract
Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in ...
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Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in human red blood cells.Methods: In this experimental laboratory study, blood samples were obtained from healthy individuals, and blood samples were divided into the control group (treated with normal saline) and 2, 4, and 6 mg/kg of sulfasalazine receiving groups. Samples treated with 2 mg/kg of sulfasalazine were treated with 2, 4, and 6 mg/kg of Pissum sativum L and Aloe vera extract, and the erythrocyte membrane stability was calculated by standard methods. Data were analyzed using a one-way analysis of variance.Results: Sulfasalazine significantly reduced the stability of the erythrocyte membrane compared to the control group. Treatment of samples with concentrations of 4 and 6 mg/kg Pissum sativum L extract significantly increased membrane stability compared to the groups treated with sulfasalazine. Treatment with 2 and 4 mg/kg of Aloe vera extract did not increase membrane stability and a 6 mg/kg of Aloe vera extract non-significantly increased the membrane stability.Conclusion: Unlike Aloe vera, Pissum sativum L extract can increase the membrane stability of red blood cells in healthy people.
Cell culture and Tissue culture
sasan Kalantari; Tahereh Naji; Rahim Ahmadi
Abstract
Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar ...
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Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar rats.Methods: In this study, 20 two-month-old adult male Wistar albino rats weighing approximately 200-150 g were studied in two groups: the control group (healthy) and the busulfan group. After this period, the testicular tissue of the mice was isolated and evaluated for the expression of Nrf-2 and Keap-1 genes, as well as changes in testosterone, FSH, and LH. Data were analyzed using SPSS software and a one-way ANOVA statistical test.Results: The statistical analysis of the results showed that the expression of Nrf-2 and Keap-1 genes in the treatment group (busulfan) decreased compared to the control group, but this decrease was not statistically significant (0.2951 and P = 0.3528). LH and testosterone levels were not significantly different between the control and treated groups (0.1327 and P = 0.0809) but FSH levels were significant between the two groups (P = 0.0189).Conclusion: The results of this study showed that busulfan can affect the level of FSH, which is effective in spermatogenesis, by causing changes in the expression of keap1 and Nrf-2 genes and causing infertility. Therefore, these genetic and hormonal factors can be used to predict infertility.
tissue engineering and Regenerative medicine
Akram Fadavi; Rahim Ahmadi; Sepideh Shahbaz Gahrouei; Maryam Hassan Nasab
Abstract
Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast ...
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Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast cells.Methods: During this experimental-laboratory study, the human neonatal foreskin was prepared and the fibroblast cells of the dermal layer were isolated and cultured. Cell viability was determined by flow cytometry and cell identity was determined using a Vimentin marker. After the preparation of chitosan hydrogel, fibroblast cells were loaded on it and the cytotoxic effect of hydrogel on cells was evaluated using the MTT assay. Data were analyzed using a t-test.Results: Cell counts showed that each dense T75 flask contained about 2 million cells. Isolated cells expressed the Vimentin marker at a high level. Fibroblasts were observed with good adhesion and uniform distribution on chitosan hydrogel. According to the results of the MTT test, the prepared hydrogel had no significant toxicity effect on fibroblast cells.Conclusion: The results of this study showed that chitosan scaffolding can be a suitable scaffold for loading fibroblast cells and transporting these cells for transplantation to damaged tissues.
Animal cell and culture
Mohammad Karamali Gilaneh; Rahim Ahmadi; Fatemeh Amini Khodashahri
Volume 2, Issue 2 , October 2021, , Pages 43-50
Abstract
Introduction: Many studies have shown the relationship between ultrasound and the function of the reproductive system. However, the mechanism of ultrasound waves action on sex hormones and testicular tissue is not perfectly clear. Accordingly, the present study investigated the effects of ultrasound ...
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Introduction: Many studies have shown the relationship between ultrasound and the function of the reproductive system. However, the mechanism of ultrasound waves action on sex hormones and testicular tissue is not perfectly clear. Accordingly, the present study investigated the effects of ultrasound waves on serum levels of DHEA-SO4, testosterone and testicular tissue in male rats.Materials and Method: Male wistar rats were divided into three groups: control group which was not exposed to ultrasound waves, rats that were exposed to ultrasound waves for 1h/day, and rats that were exposed to ultrasound waves for 6h/day. The serum levels of testosterone and DHEA-SO4 were measured using ELISA. The testicular tissue slides were prepared and analyzed microscopically.Data were analyzed using one-way ANOVA. Results: Serum levels of testosterone and DHEA-SO4 and spermatogonia, spermatocytes, sertoli cell count and seminiferous tubules morphology did not significantly changed in groups exposed to ultrasound waves compared to control animals. Conclusion: Our findings show that ultrasound waves do not significantly influence male reproductive system function and histology.
Cancer tissue and cell culture
Yeganeh Bahram Beygipour; Rahim Ahmadi; Hossein Zafari
Volume 2, Issue 1 , August 2021, , Pages 1-7
Abstract
Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was ...
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Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was to investigate the cytotoxic effects of dandelion (Taraxacum officinale) extract on cervical cancer cells compared to non-cancerous cells.Methods: In this laboratory experimental study, HeLa cancer cell line and non-cancerous embryonic kidney cells (Hek293) were purchased from Pasteur Institute, Tehran, Iran. Cells were divided into control and treatment groups. In the treatment group, cells were exposed to 0.002, 0.02, 0.2, and 2 mg/ml of dandelion flower extract. MTT assay was used to evaluate cell viability. Data were analyzed using one-way analysis of varianceResults: Hela cancer cells viability was significantly reduced in the groups exposed to 0.2 and 2 mg/ml of extract compared to the control group (p<0.01 and P<0.001, respectively). None of the concentrations used had a significant effect on the viability of Hek293 cells.Conclusion: Dandelion flower extract in appropriate concentrations can reduce the viability of cervical cancer cells without side effects on healthy non-cancerous cells. The findings of this study support previous research indicating the anticancer effects of dandelion on cancer cells.
Cell culture and Tissue culture
Sona Zare; MohammadAli Nilforoushzade; Rahim Ahmadi; Zahra Esmaeili
Volume 2, Issue 1 , August 2021, , Pages 8-14
Abstract
Introduction and Aim: Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate ...
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Introduction and Aim: Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate and evaluate the cell population of human adipose stromal vascular fraction with the main aim of investigating the presence of stem cells in human adipose stromal vascular fraction.Methods: In this laboratory-experimental study adipose tissues were obtained from 10 healthy individuals (30 to 58 years) and maintained in standard condition. After enzymatic isolation, the viability of stromal vascular fraction cells and surface antigens was evaluated by flow cytometry.Results: The results of this study showed that adipose stromal vascular fraction cells had high viability (> 98%) and were positive for CD16, CD34, CD73, CD29, CD105, CD31, and CD45 markers and negative for CD3, CD19, and CD38 markers.Conclusion: The findings of this study indicate the presence of adipose-derived stem cells, endothelial cells, peri-adipocytes, and macrophages. According to this, this fraction has potential in the clinical field of cell therapy.
