Microbiology and Microbial media
Niloofar Seyfollahi; Simin Nabizadeh; Zahra Shafiei
Abstract
Introduction and Aim: In the second half of the last century, Pseudomonas aeruginosa is considered an important hospital pathogen, according to the US Centers for Disease Control. Pseudomonas aeruginosa is the fifth pathogen among hospital microorganisms, which comprises 10% of hospital-acquired infections, ...
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Introduction and Aim: In the second half of the last century, Pseudomonas aeruginosa is considered an important hospital pathogen, according to the US Centers for Disease Control. Pseudomonas aeruginosa is the fifth pathogen among hospital microorganisms, which comprises 10% of hospital-acquired infections, as the purslane plant has a variety of vitamins and minerals in fatty acids and as a fever antiseptic medication and it is used against spasm.Material and method: To evaluate the antimicrobial activity of aqueous and ethanolic extracts of Purslane, four standard microbial methods including mixed agar diffusion method, disk diffusion, the minimum inhibitory concentration of MIC, and minimum killer concentration of MBC using the microdilution method.Results: The results showed that silver nanoparticles obtained from purslane significantly reduced the growth of Pseudomonas, however, treatments of this bacterium with only purslane extract did not cause any effects on the growth of Pseudomonas.Conclusion: It can be said that the effect of silver nanoparticles from purslane was significantly higher than the extracts extracted from purslane. pH 10 combined with 2 mM nitrate salt yielded the most suitable dimensions for silver nanoparticles, and silver nanoparticles synthesized from purslane had far greater antibacterial effect than the extract extracted from purslane. So, this nanoparticle could be a good candidate to fight infection caused by Pseudomonas aeruginosa.
