tissue engineering and Regenerative medicine
Akram Fadavi; Rahim Ahmadi; Sepideh Shahbaz Gahrouei; Maryam Hassan Nasab
Abstract
Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast ...
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Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast cells.Methods: During this experimental-laboratory study, the human neonatal foreskin was prepared and the fibroblast cells of the dermal layer were isolated and cultured. Cell viability was determined by flow cytometry and cell identity was determined using a Vimentin marker. After the preparation of chitosan hydrogel, fibroblast cells were loaded on it and the cytotoxic effect of hydrogel on cells was evaluated using the MTT assay. Data were analyzed using a t-test.Results: Cell counts showed that each dense T75 flask contained about 2 million cells. Isolated cells expressed the Vimentin marker at a high level. Fibroblasts were observed with good adhesion and uniform distribution on chitosan hydrogel. According to the results of the MTT test, the prepared hydrogel had no significant toxicity effect on fibroblast cells.Conclusion: The results of this study showed that chitosan scaffolding can be a suitable scaffold for loading fibroblast cells and transporting these cells for transplantation to damaged tissues.
