Cancer tissue and cell culture
Zainab Kamrani; Masoumeh Heshmati; Sadegh Babashah
Volume 1, Issue 3 , March 2021, , Pages 8-15
Abstract
Introduction and Aim: Silymarin, extracted from the milk thistle, is rich in flavolignans such as silybinin, silidianin, and silycristine. Several studies have been done on its anticancer effect. This study aimed to evaluate the effects of cytotoxicity and induction of silymarin-induced apoptosis on ...
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Introduction and Aim: Silymarin, extracted from the milk thistle, is rich in flavolignans such as silybinin, silidianin, and silycristine. Several studies have been done on its anticancer effect. This study aimed to evaluate the effects of cytotoxicity and induction of silymarin-induced apoptosis on colon cancer SW480 and normal HEK-293 cell lines. Methods: In the present study, the effect of cytotoxicity and apoptosis induction by silymarin on colon cancer SW480 and Normal HEK-293 cell lines were investigated by different methods such as trypan blue staining, MTT assay, Annexin V/PI staining, and evaluating the expression level of BAX and BCL2 Genes by q-PCR technique. Results: The results of the trypan blue staining and MTT assay indicated the cytotoxicity effects of silymarin in SW480 cells in a time-dependent manner. The IC50 was also evaluated. However, no cytotoxic effects were observed in HEK-293 cells after treatment with silymarin. Also, an increase of apoptosis in SW480 cells that were treated with 50 and 100 µg/mL silymarin was observed. An increase in BAX/BCL2 gene expression level was also observed in SW480 cells compared to HEK-293 cells. Conclusion: According to the results of the present study, silymarin can induce toxicity and cell apoptosis in the colon cancer cell line without causing cytotoxicity in the normal cell line. So silymarin can be suggested for effective treatment of colon cancer.
Cancer tissue and cell culture
Minoo Shahani; Farideh Firouzi
Volume 1, Issue 1 , September 2020, , Pages 26-33
Abstract
Introduction and Aim: Breast cancer is the most common cancer among Iranian women and women in the world. The aim of radiotherapy (radiation therapy) is to eliminate the maximum of cancer cells with minimal damage to healthy tissue. Intraoperative radiation therapy (IORT) can be utilized to treat a small ...
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Introduction and Aim: Breast cancer is the most common cancer among Iranian women and women in the world. The aim of radiotherapy (radiation therapy) is to eliminate the maximum of cancer cells with minimal damage to healthy tissue. Intraoperative radiation therapy (IORT) can be utilized to treat a small area of tissue without any damages. In this method, because of DNA damage and initiation of the apoptosis process, the tumor cells (target tissue) are destructed. Methods: Four Breast Cancer (BC) patients as a pilot study were treated by 21 Gy (Radical dose). Two-dimensional electrophoresis (2D-PAGE) and Progenesis Same Spots v3.2 were performed to study the proteomic of the IORT-treated tumor bed. Results: Approximately 523 protein spots were detected. A total of 343 protein spots showed different expression. among which, 141 spots decrease in-expression level, while, the remaining 191 spots were up-regulated. Conclusion: Our findings indicate that Caspase3, P53, BID, BAX, and BCL2 may be key proteins in the IORT-treated tumor bed.
