tissue engineering and Regenerative medicine
Akram Fadavi; Rahim Ahmadi; Sepideh Shahbaz Gahrouei; Maryam Hassan Nasab
Abstract
Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast ...
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Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast cells.Methods: During this experimental-laboratory study, the human neonatal foreskin was prepared and the fibroblast cells of the dermal layer were isolated and cultured. Cell viability was determined by flow cytometry and cell identity was determined using a Vimentin marker. After the preparation of chitosan hydrogel, fibroblast cells were loaded on it and the cytotoxic effect of hydrogel on cells was evaluated using the MTT assay. Data were analyzed using a t-test.Results: Cell counts showed that each dense T75 flask contained about 2 million cells. Isolated cells expressed the Vimentin marker at a high level. Fibroblasts were observed with good adhesion and uniform distribution on chitosan hydrogel. According to the results of the MTT test, the prepared hydrogel had no significant toxicity effect on fibroblast cells.Conclusion: The results of this study showed that chitosan scaffolding can be a suitable scaffold for loading fibroblast cells and transporting these cells for transplantation to damaged tissues.
Cancer tissue and cell culture
Mina Maftoon; Rahim Ahmadi
Volume 2, Issue 2 , October 2021, , Pages 28-34
Abstract
Introduction and Aim: Fibroblastoma is a common skin malignancy and a common cause of morbidity worldwide. Studies have shown that sex steroid hormones including progesterone have cytotoxic effects on cancer cells. According to this, the present study aims to determine the effects of progesterone cytotoxic ...
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Introduction and Aim: Fibroblastoma is a common skin malignancy and a common cause of morbidity worldwide. Studies have shown that sex steroid hormones including progesterone have cytotoxic effects on cancer cells. According to this, the present study aims to determine the effects of progesterone cytotoxic concentration on iNOs expression in fibroblastoma (L929) cells.Methods: Cell viability was measured using MTT assay in cells exposed to 0.001, 0.01, 0.1, 1, and 10 mg/ml of progesterone, and IC50 dose was determined. NO concentration levels were also measured using the Griess test. The expression level of iNOS was measured by real-time RT-PCR. Data were analyzed using SPSS by one-way ANOVA.Results: Viability significantly decreased in fibroblastoma cells exposed to 1 and 10 mg/ml of progesterone (P<0.001). The relative expression level of iNOS significantly increased in cells exposed to IC50 dose of progesterone (P<0.001). The relative concentration of NO significantly increased in fibroblastoma cells exposed to 0.01, 0.1, and 1 mg/ml of progesterone (P<0.001, P<0.001, and P<0.05, respectively).Conclusion: Progesterone has cytotoxic effects on fibroblastoma cancer cells due partly to the effects of the hormone on iNOS expression level and increased NO that probably induces apoptosis in fibroblastoma cancer cells.
Cancer tissue and cell culture
Yeganeh Bahram Beygipour; Rahim Ahmadi; Hossein Zafari
Volume 2, Issue 1 , August 2021, , Pages 1-7
Abstract
Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was ...
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Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was to investigate the cytotoxic effects of dandelion (Taraxacum officinale) extract on cervical cancer cells compared to non-cancerous cells.Methods: In this laboratory experimental study, HeLa cancer cell line and non-cancerous embryonic kidney cells (Hek293) were purchased from Pasteur Institute, Tehran, Iran. Cells were divided into control and treatment groups. In the treatment group, cells were exposed to 0.002, 0.02, 0.2, and 2 mg/ml of dandelion flower extract. MTT assay was used to evaluate cell viability. Data were analyzed using one-way analysis of varianceResults: Hela cancer cells viability was significantly reduced in the groups exposed to 0.2 and 2 mg/ml of extract compared to the control group (p<0.01 and P<0.001, respectively). None of the concentrations used had a significant effect on the viability of Hek293 cells.Conclusion: Dandelion flower extract in appropriate concentrations can reduce the viability of cervical cancer cells without side effects on healthy non-cancerous cells. The findings of this study support previous research indicating the anticancer effects of dandelion on cancer cells.
