Plant Cell Culture and Tissue
Somayyeh Kheiri; Alameh Babajani
Abstract
Background and aim: This study was performed to identify the anatomical responses of lemon balm to selenium nanoparticles (nSe).Methods: This experimental laboratory study was done at the concentrations of 10 and 50 mg/L of nSe.Results: Plants were treated with nano-selenium (nSe; 0, 10 and 50 mg/l) ...
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Background and aim: This study was performed to identify the anatomical responses of lemon balm to selenium nanoparticles (nSe).Methods: This experimental laboratory study was done at the concentrations of 10 and 50 mg/L of nSe.Results: Plants were treated with nano-selenium (nSe; 0, 10 and 50 mg/l) and bulk selenium (BSe). The results of this study indicated that nano selenium had a positive and beneficial effect at a concentration of 10, but a concentration of 50 induced toxicity. The microscopic assessment of stem cross-sections showed that the diameter of the central cylinder was increased compared to the control. The highest increase was related to the seedlings exposed to the 50 and 10 mg/l. Stem thickness and diameter in the nSe-treated seedlings at 50 mg/l and bulk at 10 mg/l were increased compared to the control. Xylem diameter decreased in response to the supplements, the lowest belongs to the nSe50. Monitoring the leaf cross-sections showed that nSe10 increased the thickness of xylem diameter, palisade, and sponge parenchyma compared to the control. A comparison of petiole cross-sections indicated that the thickness of the epidermis and the diameter of the central and lateral xylem were changed. While 50 mg/l nSe declined these traits.Conclusion: Therefore, the results of this study indicate that the application of applied concentration-dependent selenium, in addition to inducing physiological and molecular changes, causes anatomical changes and tissue differentiation. Further research is needed in the future to determine the mechanism of these responses.
Plant Cell Culture and Tissue
Leila Zarandi-Miandoab; Zahra Oraghi Ardebili
Abstract
To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases ...
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To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases in root and shoot fresh weights, and chlorophyll content. While, the nSe at 50 mgl-1 exhibited severe phyotoxicity. Also, nSe10 enhanced uptake and accumulation of Ca and Mg in both leaf and root, contrasted to the nSe50-treated plants. The applied supplements modified phenylalanine ammonia lyase activity, concentrations of flavonids, glutathione, and proline. Moreover, these supplements in the dose and type-dependent manners changed the activities of catalase. Furthermore, the applied treatments up-regulated the expression of phenylalanine ammonia-lyase (PAL) and Coumarate: CoA-ligase (4CL) genes. The comparative physiological and molecular evidence on phytotoxicity and potential advantages of nSe and its bulk counterpart was provided as a theoretical basis for exploiting in food, agricultural, and pharmaceutical industries.
