Cancer tissue and cell culture
Maryam Bikhof Torbati; Masoud Shaabanzadeh; Mahzad Motallebi
Volume 1, Issue 3 , March 2021, , Pages 1-7
Abstract
Introduction and Aim: Drug nanocarriers have been used extensively in cancer therapy due to their features like the ability to targeting drug transmission, increasing drug solubility, and reducing the drug cytotoxic effects on healthy tissues. The purpose of this study was to investigate the effects ...
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Introduction and Aim: Drug nanocarriers have been used extensively in cancer therapy due to their features like the ability to targeting drug transmission, increasing drug solubility, and reducing the drug cytotoxic effects on healthy tissues. The purpose of this study was to investigate the effects of Tamoxifen nanocapsules on the expression of Bax and Bak genes in MCF-7 cell lines. Methods: In this study, the nanocapsule structure was confirmed by FTIR spectroscopy and the effects of Tamoxifen nanocapsules on cell bioactivity were evaluated by MTT assay at concentrations of 10, 50, 100, and 200 µg/mL after 48 hours. Real-time PCR was used to analyze the expression of Bax and Bak genes and data were analyzed using SPSS (23.0) software. Results: According to the MTT assay, higher concentrations of Tamoxifen nanocapsules decreased cell bioactivity in a dose-dependent manner and the highest toxicity of nanocapsules was at the concentration of 200 µg/mL. The expression level of Bax and Bak genes in MCF-7 treated cells after 48 hours indicated the induction of apoptosis in cells. The results revealed a 1.8-fold increase in cytotoxicity of Tamoxifen nanocapsules compared to free Tamoxifen. Conclusion: The apoptosis induction as a result of increased expression of Bax and Bak genes and enhanced cytotoxicity, makes Tamoxifen nanocapsules a promising treatment for breast cancer therapy compared to free Tamoxifen.
Medical Genetics
Flora Forouzesh; Pantea Hajimirza Shafiesoltani; Mahsa Ghiaghi; Mahdi Shabani
Volume 1, Issue 3 , March 2021, , Pages 16-24
Abstract
Introduction and Aim: Colorectal cancer is one of the most common cancers. Epigenetic change has been considered by many scientists as a therapeutic target. Hyper acetylation of chromatin components by sodium butyrate can alter gene regulation. This study aims to investigate the effects of sodium butyrate ...
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Introduction and Aim: Colorectal cancer is one of the most common cancers. Epigenetic change has been considered by many scientists as a therapeutic target. Hyper acetylation of chromatin components by sodium butyrate can alter gene regulation. This study aims to investigate the effects of sodium butyrate on Bax and Bcl-2 gene expression. Methods: Caco-2 cell line was treated with different concentrations of sodium butyrate (25 mM to 150 mM) based on IC50 concentration in two time periods of 24 hours and 48 hours. Bax and Bcl-2 gene expression were measured by qReal-Time PCR technique and Bcl2/Bax ratio was evaluated. Results: The results showed that sodium butyrate increased the expression of Bax gene and decreased the expression of Bcl-2 gene in treated cells compared to the control group, which was statistically significant (p < /em> <0.05), and 25 mM was selected as the most effective dose after 48 hours of treatment. Also, the Bcl-2/Bax ratio at the same concentration showed a significant decrease Conclusion: Sodium butyrate induces apoptosis in cancer cells by reducing the expression ratio of Bcl-2/Bax. It can be used as a therapeutic target but needs further investigation.
