Plant Cell Culture and Tissue
Alireza Iranbakhsh; Marjan Assefi
Plant Cell Culture and Tissue
Leila Zarandi-Miandoab; Zahra Oraghi Ardebili
Abstract
To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases ...
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To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases in root and shoot fresh weights, and chlorophyll content. While, the nSe at 50 mgl-1 exhibited severe phyotoxicity. Also, nSe10 enhanced uptake and accumulation of Ca and Mg in both leaf and root, contrasted to the nSe50-treated plants. The applied supplements modified phenylalanine ammonia lyase activity, concentrations of flavonids, glutathione, and proline. Moreover, these supplements in the dose and type-dependent manners changed the activities of catalase. Furthermore, the applied treatments up-regulated the expression of phenylalanine ammonia-lyase (PAL) and Coumarate: CoA-ligase (4CL) genes. The comparative physiological and molecular evidence on phytotoxicity and potential advantages of nSe and its bulk counterpart was provided as a theoretical basis for exploiting in food, agricultural, and pharmaceutical industries.
Cancer tissue and cell culture
Ali Asghar Azizi Jirabadi; Seyed Ataollah Sadat Shandiz; Maryam Abbasi
Volume 1, Issue 2 , December 2020, , Pages 15-20
Abstract
Introduction and Aim: Breast Cancer is the most prevalent type of tumor and is also have second major death among women. The aim of the current study was to investigate the anticancer properties of Phormidiumm Animale cyanobacterium extract using MTT assay and through modulation of p53 gene expression ...
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Introduction and Aim: Breast Cancer is the most prevalent type of tumor and is also have second major death among women. The aim of the current study was to investigate the anticancer properties of Phormidiumm Animale cyanobacterium extract using MTT assay and through modulation of p53 gene expression in breast cancer cells. Methods: In the current study, the breast cancer MCF-7 and normal HEK293 cell lines were treated with various concentrations of P. animale extract ranging 50, 100, 200 mg/mL for 24 hours. Cell viability was measured using MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay against cell lines. The gene expressions of p53 compared with GAPDH were measured using the real-time PCR method. Results: The MTT results showed that P. animale significantly decreased the viability of cancer cells compared to normal cells in dose-dependent mode. Moreover, the mRNA levels of p53 were significantly increased from 3.34 in MCF-7 cells to 5.15 and 9.11 fold, respectively, following treatment with 50, 100, and 200 mg/mL P. animale extract. Conclusion: The data suggest that P. animale extract can induce cytotoxicity and might modulate apoptosis by up-regulating p53 gene expression in human breast cancer MCF-7 cell line.
