Medical Genetics
Flora Forouzesh; Pantea Hajimirza Shafiesoltani; Mahsa Ghiaghi; Mahdi Shabani
Volume 1, Issue 3 , March 2021, , Pages 16-24
Abstract
Introduction and Aim: Colorectal cancer is one of the most common cancers. Epigenetic change has been considered by many scientists as a therapeutic target. Hyper acetylation of chromatin components by sodium butyrate can alter gene regulation. This study aims to investigate the effects of sodium butyrate ...
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Introduction and Aim: Colorectal cancer is one of the most common cancers. Epigenetic change has been considered by many scientists as a therapeutic target. Hyper acetylation of chromatin components by sodium butyrate can alter gene regulation. This study aims to investigate the effects of sodium butyrate on Bax and Bcl-2 gene expression. Methods: Caco-2 cell line was treated with different concentrations of sodium butyrate (25 mM to 150 mM) based on IC50 concentration in two time periods of 24 hours and 48 hours. Bax and Bcl-2 gene expression were measured by qReal-Time PCR technique and Bcl2/Bax ratio was evaluated. Results: The results showed that sodium butyrate increased the expression of Bax gene and decreased the expression of Bcl-2 gene in treated cells compared to the control group, which was statistically significant (p < /em> <0.05), and 25 mM was selected as the most effective dose after 48 hours of treatment. Also, the Bcl-2/Bax ratio at the same concentration showed a significant decrease Conclusion: Sodium butyrate induces apoptosis in cancer cells by reducing the expression ratio of Bcl-2/Bax. It can be used as a therapeutic target but needs further investigation.
Medical Genetics
Hybert keshishian; Shadi Habibnia; Mohamad Reza Fallahian; Saeed Zaker Bostanabad
Volume 1, Issue 3 , March 2021, , Pages 33-39
Abstract
Introduction and aim: The hepatitis B virus (HBV) was discovered in 1965. Approximately 350 million people are infected with HBV. The virus has a worldwide spread and one of the most important complications of infection is chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Hepatitis can be detected ...
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Introduction and aim: The hepatitis B virus (HBV) was discovered in 1965. Approximately 350 million people are infected with HBV. The virus has a worldwide spread and one of the most important complications of infection is chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Hepatitis can be detected by biochemical tests that determine liver function. Methods: This study was qualitative and 30 patients with suspected hepatitis B referring to Massoud Lab in 2019 were selected. Blood samples were tested for HBsAg positive by multiplex RT-PCR with specific probes and primers as well as β-Actin gene as quality control. Then the obtained results were compared with the results of commercial tests. Results: From 30 samples, 19 samples were HBsAg positive and 11 samples were HBsAg negative. Positive samples were re-examined to confirm the positive results. To compare and evaluate the quality of the results of the designed test, all of these 30 samples were analyzed using another common laboratory kit. Conclusion: The high sensitivity, good reproducibility, and low cost make this innovative quantitative HBV real-time PCR assay especially well-suited for application to large clinical surveys.
Medical Genetics
Saeed Zaker Bostanabad; Soheila Darmanlo; Somayeh Darmanlo
Volume 1, Issue 2 , December 2020, , Pages 35-40
Abstract
Introduction and aim: Recurrent miscarriage is two or more consecutive pregnancy losses before weeks 20. Different factors involved in recurrent abortion as a multifactorial disease. Genetic factors are one of the important factors associated with recurrent abortion. The goal of this article is to assess ...
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Introduction and aim: Recurrent miscarriage is two or more consecutive pregnancy losses before weeks 20. Different factors involved in recurrent abortion as a multifactorial disease. Genetic factors are one of the important factors associated with recurrent abortion. The goal of this article is to assess the association between single nucleotide polymorphisms of TGFB1 and JAK2 polymorphism and the recurrent miscarriage in Iranian women. Methods: The study group was 30 women with recurrent miscarriages with unknown reason and the control groups consisted of 10 women with at least two successful pregnancies and no miscarriages. PCR was used to find the association between TGFB1 and JAK2 genes and recurrent miscarriages. Analysing performed with FinchTV software after data sequencing. Results: Our results provided evidence that TGFB1 polymorphisms influence the risk of recurrent abortion in the Iranian population however there is no correlation with JAK2 polymorphism and recurrent abortion. Conclusion: This finding could help to prevent recurrent miscarriage in Iranian women.
Medical Genetics
Saeed Zaker Bostanabad; Soheila Darmanlo; Somayeh Darmanloo
Volume 1, Issue 1 , September 2020, , Pages 9-15
Abstract
Introduction and Aim: According to the WHO definition, recurrent miscarriage is a consecutive pregnancy loss before weeks 22. Different factors involved in recurrent abortion as a multifactorial disease. Genetic factors are one of the important factors associated with recurrent abortion. VEGFA (Vascular ...
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Introduction and Aim: According to the WHO definition, recurrent miscarriage is a consecutive pregnancy loss before weeks 22. Different factors involved in recurrent abortion as a multifactorial disease. Genetic factors are one of the important factors associated with recurrent abortion. VEGFA (Vascular Endothelial Growth Factor A) is one of the important gene in angiogenesis. MTHFD1 (Methylenetetrahydrofolate Dehydrogenase, Cyclohydrolase, And Formyltetrahydrofolate Synthetase 1) is a protein-coding gene that metabolism folate. The goal of this article is to assess the association between single nucleotide polymorphisms of VEGFA and MTHFD1 and the recurrent miscarriage in Iranian women.Material and method: The study group was 50 women with recurrent miscarriages and the control groups consisted of 10 women with at least two successful pregnancies and no miscarriages. The DNA extracted from leucocytes cells. PCR was used to find the association between VEGFA and MTHFD1 genes and recurrent miscarriages. Analyzing performed with FinchTV software after data sequencing.Result: Our results showed that the polymorphism frequency of CC and CT in rs2236225 VEGFA were 86% and 14% in women with recurrent miscarriages and 90% and 10% in the control group. the polymorphism frequency of GG and GA in rs3025039MTHFD1 were 82% and 18% in women with recurrent miscarriages and no polymorphism was detected in the control group.Conclusion: These results showed that there is an association between single nucleotide polymorphisms of VEGFA and MTHFD1 and the recurrent miscarriage in Iranian women. Furthermore, MTHFD1 polymorphism could be a molecular marker in the diagnosis of recurrent miscarriage.
Medical Genetics
Ani Dejakam; Ani Dejakam; Azadeh Hekmat
Volume 1, Issue 1 , September 2020, , Pages 16-25
Abstract
Introduction and Aim: Opium addiction is one of the most prevalent addiction in Iranian society. Throughout the last two decades, congress 60; a nongovernmental organization; has been operated a taper off treatment of opium associated with a package of psychological treatment group classes. Although ...
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Introduction and Aim: Opium addiction is one of the most prevalent addiction in Iranian society. Throughout the last two decades, congress 60; a nongovernmental organization; has been operated a taper off treatment of opium associated with a package of psychological treatment group classes. Although the effectiveness of the taper off method in opium addiction has been verified, molecular mechanisms involved in this treatment have not explained. BDNF gene is a brain-derived neurotrophic factor that is associated with numerous molecular mechanisms of the brain including memory. Methods: In this research, peripheral blood samples from 21 patients were collected from Congress 60. RNA was extracted from each sample, reverse transcribed and amplified via RT-PCR technique, utilizing specific primers for BDNF. The production of BDNF protein was also analyzed employing the western blotting technique. Results: Our results exhibited considerable down expression of BDNF in addict persons vs. non-psychiatric persons (p < /em><0.01). Furthermore, BDNF expression levels in addicts increased significantly after the therapy period. Findings showed the effect of opium abuse and taper off treatment on the expression of BDNF. Conclusion: The present study could help to a better understanding of molecular and neurological mechanisms of opium and taper off treatment. Additionally, BDNF level detection could be a potential marker for screening the effectiveness of various types of addiction treatment.
