Cancer tissue and cell culture
Kimia Farokhi Bahar; Azadeh Hekmat
Abstract
Introduction and aim: Palm oil (PO) is the second largest vegetable oil in the world and contains large quantities of phytochemicals, for example, vitamin E, carotenoids, and phytosterols. This study aimed to investigate the effect of Palm oil on the cellular growth of K652 blood cancer, as a model of ...
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Introduction and aim: Palm oil (PO) is the second largest vegetable oil in the world and contains large quantities of phytochemicals, for example, vitamin E, carotenoids, and phytosterols. This study aimed to investigate the effect of Palm oil on the cellular growth of K652 blood cancer, as a model of cancer cells.Methods: In this study, K562 leukemia cells were treated with concentrations of 5, 25, 50, 75, and 100 µg per mL of PO for 24 and 48 hours. MTT assay, NAC assay, and microscopic observations were used to evaluate cell changes and cell death/survival.Results: PO at concentrations above 5 μg per ml induced significant cell death. Microscopic observations also confirmed cell death in cells that were treated with PO. Also, the rate of inhibition of cell growth of PO after 24 hours was more than 48 hours. After NAC treatment, 81% cell survival was observed (P <0.001).Conclusion: According to the results of this study, PO has a great inhibitory effect on K562 leukemia cells and could be a good candidate for cancer therapy.
Cancer tissue and cell culture
Mahdi Azadmanesh; Tahereh Naji; Abdolreza Mohammadnia
Abstract
Introduction and aim: Genes play a very important role in the development of cancer. This study aimed to evaluate three very important genes (C-fos, C-jun, and P53) in Wistar mice's stomach tissue cancer cells designed by dimethylhydrazine.Methods: In this study, forty male Wistar mice, 57 weeks old, ...
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Introduction and aim: Genes play a very important role in the development of cancer. This study aimed to evaluate three very important genes (C-fos, C-jun, and P53) in Wistar mice's stomach tissue cancer cells designed by dimethylhydrazine.Methods: In this study, forty male Wistar mice, 57 weeks old, were evaluated and divided into four groups of Ten. In the control group, the second group received Cinnamaldehyde-free carcinogens, the third group was healthy with the Cinnamaldehyde, and the fourth group received carcinogens with the Cinnamaldehyde at the same time. Then, the gastric tissue of mice was isolated and evaluated for the expression of C-fos, C-Jun, and P53 genes. The data were analyzed with One-way ANOVA using SPSS.Results: Statistical analysis of the results showed that the expression of C-fos, C-Jun, and P53 genes in the carcinogen receptor with the Cinnamaldehyde was increased compared to the control group. This increase was statistically significant for two genes C-fos (P = 0.0146) and P53 (P = 0.0212) and for the C-Jun gene (P = 0.01604) this increase was not significant.Conclusion: The results of this study showed that Cinnamaldehyde can alter the expression of important genes C-fos, C-Jun, and P53 that are effective in causing gastric cancer.
Cancer tissue and cell culture
Mina Maftoon; Rahim Ahmadi
Volume 2, Issue 2 , October 2021, , Pages 28-34
Abstract
Introduction and Aim: Fibroblastoma is a common skin malignancy and a common cause of morbidity worldwide. Studies have shown that sex steroid hormones including progesterone have cytotoxic effects on cancer cells. According to this, the present study aims to determine the effects of progesterone cytotoxic ...
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Introduction and Aim: Fibroblastoma is a common skin malignancy and a common cause of morbidity worldwide. Studies have shown that sex steroid hormones including progesterone have cytotoxic effects on cancer cells. According to this, the present study aims to determine the effects of progesterone cytotoxic concentration on iNOs expression in fibroblastoma (L929) cells.Methods: Cell viability was measured using MTT assay in cells exposed to 0.001, 0.01, 0.1, 1, and 10 mg/ml of progesterone, and IC50 dose was determined. NO concentration levels were also measured using the Griess test. The expression level of iNOS was measured by real-time RT-PCR. Data were analyzed using SPSS by one-way ANOVA.Results: Viability significantly decreased in fibroblastoma cells exposed to 1 and 10 mg/ml of progesterone (P<0.001). The relative expression level of iNOS significantly increased in cells exposed to IC50 dose of progesterone (P<0.001). The relative concentration of NO significantly increased in fibroblastoma cells exposed to 0.01, 0.1, and 1 mg/ml of progesterone (P<0.001, P<0.001, and P<0.05, respectively).Conclusion: Progesterone has cytotoxic effects on fibroblastoma cancer cells due partly to the effects of the hormone on iNOS expression level and increased NO that probably induces apoptosis in fibroblastoma cancer cells.
Cancer tissue and cell culture
Yeganeh Bahram Beygipour; Rahim Ahmadi; Hossein Zafari
Volume 2, Issue 1 , August 2021, , Pages 1-7
Abstract
Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was ...
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Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was to investigate the cytotoxic effects of dandelion (Taraxacum officinale) extract on cervical cancer cells compared to non-cancerous cells.Methods: In this laboratory experimental study, HeLa cancer cell line and non-cancerous embryonic kidney cells (Hek293) were purchased from Pasteur Institute, Tehran, Iran. Cells were divided into control and treatment groups. In the treatment group, cells were exposed to 0.002, 0.02, 0.2, and 2 mg/ml of dandelion flower extract. MTT assay was used to evaluate cell viability. Data were analyzed using one-way analysis of varianceResults: Hela cancer cells viability was significantly reduced in the groups exposed to 0.2 and 2 mg/ml of extract compared to the control group (p<0.01 and P<0.001, respectively). None of the concentrations used had a significant effect on the viability of Hek293 cells.Conclusion: Dandelion flower extract in appropriate concentrations can reduce the viability of cervical cancer cells without side effects on healthy non-cancerous cells. The findings of this study support previous research indicating the anticancer effects of dandelion on cancer cells.
Cancer tissue and cell culture
Hossein Gravand; Jafar Kazemian; Masoumeh Heshmati
Volume 2, Issue 1 , August 2021, , Pages 21-27
Abstract
Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis ...
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Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis induction of 7-hydroxycycoumarin were investigated on the SW480 colon cancer cell line.Methods: anti-proliferative effect of 7-hydroxycoumarin was investigated using Trypan blue and MTT assay at different concentrations on SW480 cell line. Additionally, apoptosis induction was examined by flow cytometry and real-time PCR.Results: The results show a decrease in viability. IC50 concentrations were observed at the concentrations of 164.2, 80.48, and 41.97 μg/ml for 24, 48, and 72 h, respectively. Of note, IC50 concentrations were not observed in the trypan blue assay. As well, there was a 2.4-fold increase in apoptosis (P<0.001) using Annexin / PI kit by flow cytometry. as well as the increased expression of BAX/BCL2 ratio of about 13.69, which was observed at 100 μg/ml compared to the control.Discussion: The results indicate that 7-hydroxycoumarin could inhibit cell proliferation and induce apoptosis in the SW480 cell line. Therefore, 7-hydroxycycoumarin can be considered as an anti-cancer agent in the complementary treatment of colon cancer.
Cancer tissue and cell culture
Maryam Bikhof Torbati; Masoud Shaabanzadeh; Mahzad Motallebi
Volume 1, Issue 3 , March 2021, , Pages 1-7
Abstract
Introduction and Aim: Drug nanocarriers have been used extensively in cancer therapy due to their features like the ability to targeting drug transmission, increasing drug solubility, and reducing the drug cytotoxic effects on healthy tissues. The purpose of this study was to investigate the effects ...
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Introduction and Aim: Drug nanocarriers have been used extensively in cancer therapy due to their features like the ability to targeting drug transmission, increasing drug solubility, and reducing the drug cytotoxic effects on healthy tissues. The purpose of this study was to investigate the effects of Tamoxifen nanocapsules on the expression of Bax and Bak genes in MCF-7 cell lines. Methods: In this study, the nanocapsule structure was confirmed by FTIR spectroscopy and the effects of Tamoxifen nanocapsules on cell bioactivity were evaluated by MTT assay at concentrations of 10, 50, 100, and 200 µg/mL after 48 hours. Real-time PCR was used to analyze the expression of Bax and Bak genes and data were analyzed using SPSS (23.0) software. Results: According to the MTT assay, higher concentrations of Tamoxifen nanocapsules decreased cell bioactivity in a dose-dependent manner and the highest toxicity of nanocapsules was at the concentration of 200 µg/mL. The expression level of Bax and Bak genes in MCF-7 treated cells after 48 hours indicated the induction of apoptosis in cells. The results revealed a 1.8-fold increase in cytotoxicity of Tamoxifen nanocapsules compared to free Tamoxifen. Conclusion: The apoptosis induction as a result of increased expression of Bax and Bak genes and enhanced cytotoxicity, makes Tamoxifen nanocapsules a promising treatment for breast cancer therapy compared to free Tamoxifen.
Cancer tissue and cell culture
Zainab Kamrani; Masoumeh Heshmati; Sadegh Babashah
Volume 1, Issue 3 , March 2021, , Pages 8-15
Abstract
Introduction and Aim: Silymarin, extracted from the milk thistle, is rich in flavolignans such as silybinin, silidianin, and silycristine. Several studies have been done on its anticancer effect. This study aimed to evaluate the effects of cytotoxicity and induction of silymarin-induced apoptosis on ...
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Introduction and Aim: Silymarin, extracted from the milk thistle, is rich in flavolignans such as silybinin, silidianin, and silycristine. Several studies have been done on its anticancer effect. This study aimed to evaluate the effects of cytotoxicity and induction of silymarin-induced apoptosis on colon cancer SW480 and normal HEK-293 cell lines. Methods: In the present study, the effect of cytotoxicity and apoptosis induction by silymarin on colon cancer SW480 and Normal HEK-293 cell lines were investigated by different methods such as trypan blue staining, MTT assay, Annexin V/PI staining, and evaluating the expression level of BAX and BCL2 Genes by q-PCR technique. Results: The results of the trypan blue staining and MTT assay indicated the cytotoxicity effects of silymarin in SW480 cells in a time-dependent manner. The IC50 was also evaluated. However, no cytotoxic effects were observed in HEK-293 cells after treatment with silymarin. Also, an increase of apoptosis in SW480 cells that were treated with 50 and 100 µg/mL silymarin was observed. An increase in BAX/BCL2 gene expression level was also observed in SW480 cells compared to HEK-293 cells. Conclusion: According to the results of the present study, silymarin can induce toxicity and cell apoptosis in the colon cancer cell line without causing cytotoxicity in the normal cell line. So silymarin can be suggested for effective treatment of colon cancer.
Cancer tissue and cell culture
Ali Asghar Azizi Jirabadi; Seyed Ataollah Sadat Shandiz; Maryam Abbasi
Volume 1, Issue 2 , December 2020, , Pages 15-20
Abstract
Introduction and Aim: Breast Cancer is the most prevalent type of tumor and is also have second major death among women. The aim of the current study was to investigate the anticancer properties of Phormidiumm Animale cyanobacterium extract using MTT assay and through modulation of p53 gene expression ...
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Introduction and Aim: Breast Cancer is the most prevalent type of tumor and is also have second major death among women. The aim of the current study was to investigate the anticancer properties of Phormidiumm Animale cyanobacterium extract using MTT assay and through modulation of p53 gene expression in breast cancer cells. Methods: In the current study, the breast cancer MCF-7 and normal HEK293 cell lines were treated with various concentrations of P. animale extract ranging 50, 100, 200 mg/mL for 24 hours. Cell viability was measured using MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay against cell lines. The gene expressions of p53 compared with GAPDH were measured using the real-time PCR method. Results: The MTT results showed that P. animale significantly decreased the viability of cancer cells compared to normal cells in dose-dependent mode. Moreover, the mRNA levels of p53 were significantly increased from 3.34 in MCF-7 cells to 5.15 and 9.11 fold, respectively, following treatment with 50, 100, and 200 mg/mL P. animale extract. Conclusion: The data suggest that P. animale extract can induce cytotoxicity and might modulate apoptosis by up-regulating p53 gene expression in human breast cancer MCF-7 cell line.
Cancer tissue and cell culture
Minoo Shahani; Farideh Firouzi
Volume 1, Issue 1 , September 2020, , Pages 26-33
Abstract
Introduction and Aim: Breast cancer is the most common cancer among Iranian women and women in the world. The aim of radiotherapy (radiation therapy) is to eliminate the maximum of cancer cells with minimal damage to healthy tissue. Intraoperative radiation therapy (IORT) can be utilized to treat a small ...
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Introduction and Aim: Breast cancer is the most common cancer among Iranian women and women in the world. The aim of radiotherapy (radiation therapy) is to eliminate the maximum of cancer cells with minimal damage to healthy tissue. Intraoperative radiation therapy (IORT) can be utilized to treat a small area of tissue without any damages. In this method, because of DNA damage and initiation of the apoptosis process, the tumor cells (target tissue) are destructed. Methods: Four Breast Cancer (BC) patients as a pilot study were treated by 21 Gy (Radical dose). Two-dimensional electrophoresis (2D-PAGE) and Progenesis Same Spots v3.2 were performed to study the proteomic of the IORT-treated tumor bed. Results: Approximately 523 protein spots were detected. A total of 343 protein spots showed different expression. among which, 141 spots decrease in-expression level, while, the remaining 191 spots were up-regulated. Conclusion: Our findings indicate that Caspase3, P53, BID, BAX, and BCL2 may be key proteins in the IORT-treated tumor bed.
