Cell culture and Tissue culture
Shahin Seyf; Rahim Ahmadi; Sara Najafi
Abstract
Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in ...
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Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in human red blood cells.Methods: In this experimental laboratory study, blood samples were obtained from healthy individuals, and blood samples were divided into the control group (treated with normal saline) and 2, 4, and 6 mg/kg of sulfasalazine receiving groups. Samples treated with 2 mg/kg of sulfasalazine were treated with 2, 4, and 6 mg/kg of Pissum sativum L and Aloe vera extract, and the erythrocyte membrane stability was calculated by standard methods. Data were analyzed using a one-way analysis of variance.Results: Sulfasalazine significantly reduced the stability of the erythrocyte membrane compared to the control group. Treatment of samples with concentrations of 4 and 6 mg/kg Pissum sativum L extract significantly increased membrane stability compared to the groups treated with sulfasalazine. Treatment with 2 and 4 mg/kg of Aloe vera extract did not increase membrane stability and a 6 mg/kg of Aloe vera extract non-significantly increased the membrane stability.Conclusion: Unlike Aloe vera, Pissum sativum L extract can increase the membrane stability of red blood cells in healthy people.
Cell culture and Tissue culture
sasan Kalantari; Tahereh Naji; Rahim Ahmadi
Abstract
Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar ...
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Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar rats.Methods: In this study, 20 two-month-old adult male Wistar albino rats weighing approximately 200-150 g were studied in two groups: the control group (healthy) and the busulfan group. After this period, the testicular tissue of the mice was isolated and evaluated for the expression of Nrf-2 and Keap-1 genes, as well as changes in testosterone, FSH, and LH. Data were analyzed using SPSS software and a one-way ANOVA statistical test.Results: The statistical analysis of the results showed that the expression of Nrf-2 and Keap-1 genes in the treatment group (busulfan) decreased compared to the control group, but this decrease was not statistically significant (0.2951 and P = 0.3528). LH and testosterone levels were not significantly different between the control and treated groups (0.1327 and P = 0.0809) but FSH levels were significant between the two groups (P = 0.0189).Conclusion: The results of this study showed that busulfan can affect the level of FSH, which is effective in spermatogenesis, by causing changes in the expression of keap1 and Nrf-2 genes and causing infertility. Therefore, these genetic and hormonal factors can be used to predict infertility.
Cell culture and Tissue culture
Arefeh Kamali; Abbas Zare MirakAbadi
Volume 2, Issue 2 , October 2021, , Pages 21-27
Abstract
Introduction and Aim: Many colubrid snakes produce toxic oral secretions and some species have caused severe reactions in humans. In this research toxicity of Duvernoy´s gland secretion (DGS) from Hemorrhois ravergieri (aglyph) has been studied.Methods: In this study, the effect of Hemorrhois ravergieri ...
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Introduction and Aim: Many colubrid snakes produce toxic oral secretions and some species have caused severe reactions in humans. In this research toxicity of Duvernoy´s gland secretion (DGS) from Hemorrhois ravergieri (aglyph) has been studied.Methods: In this study, the effect of Hemorrhois ravergieri saliva on human dermal fibroblasts (HDF) cells growth was determined by the inverted microscope and MTT assay. The integrity of the cell membrane through LDH release was evaluated as well.Results: The MTT assay and Neutral red assay showed a significant (p˂0.05) cytotoxic effect of Hemorrhois ravergieri salvia on HDF cells growth after 24 h treatment. Additionally, Hemorrhois ravergieri venom caused a significant increase in LDH release (p˂0.05). Numerous morphological abnormalities were observed in cells exposed to the DGS and showed loss of their common polygonal shape, appearing as several roughly rounded cells of variable size. Conclusion: The Hemorrhois ravergieri saliva causes cytotoxic effects on HDF cells by the necrotic mechanism. This colubrid snake bite causes local symptoms, so this research suggests a more careful evaluation of the victims when considering the medical treatment to be adopted.
Cell culture and Tissue culture
Sahar Salmanzadeh Zehkesh; Naser Mohammadpour Donighi
Volume 2, Issue 2 , October 2021, , Pages 35-42
Abstract
Introduction and Aim: Alginate nanoparticles have been used in novel drug delivery systems due to marvelous features including biocompatibility, biodegradable, mucoadhesive, and non-toxic. At present various types of adjuvants are used against snake venom, as a solution, suspension, or emulsion systems, ...
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Introduction and Aim: Alginate nanoparticles have been used in novel drug delivery systems due to marvelous features including biocompatibility, biodegradable, mucoadhesive, and non-toxic. At present various types of adjuvants are used against snake venom, as a solution, suspension, or emulsion systems, which are used in different centers. The purpose of this study is to design a novel type of antigen delivery system as an adjuvant from alginate nanoparticles.Methods: Alginate nanoparticles were prepared based on the ionic gelation process. The nanoparticles' particle size, morphology, zeta potential, in vitro release behavior, and structure of nanoparticles contained venom were analyzed by SEM, DLS, and FT-IR spectroscopy. Cell viability of SW-480 cells was measured using an MTT assay.Results: The particles were spherical. The optimum particles size of alginate and venom-loaded nanoparticles were 118 and 169 nm, respectively. Viability significantly decreased in cells exposed to upper 20 µg/ml o (P<0.05).Conclusion: Our results suggested that alginate nanoparticles, which were prepared in our study possibly, could be used as an alternative for traditional adjuvant systems.
Cell culture and Tissue culture
Sona Zare; MohammadAli Nilforoushzade; Rahim Ahmadi; Zahra Esmaeili
Volume 2, Issue 1 , August 2021, , Pages 8-14
Abstract
Introduction and Aim: Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate ...
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Introduction and Aim: Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate and evaluate the cell population of human adipose stromal vascular fraction with the main aim of investigating the presence of stem cells in human adipose stromal vascular fraction.Methods: In this laboratory-experimental study adipose tissues were obtained from 10 healthy individuals (30 to 58 years) and maintained in standard condition. After enzymatic isolation, the viability of stromal vascular fraction cells and surface antigens was evaluated by flow cytometry.Results: The results of this study showed that adipose stromal vascular fraction cells had high viability (> 98%) and were positive for CD16, CD34, CD73, CD29, CD105, CD31, and CD45 markers and negative for CD3, CD19, and CD38 markers.Conclusion: The findings of this study indicate the presence of adipose-derived stem cells, endothelial cells, peri-adipocytes, and macrophages. According to this, this fraction has potential in the clinical field of cell therapy.
Toxicology
Abbas Zare MirakAbadi; Parvin Horrieh
Volume 1, Issue 1 , September 2020, , Pages 1-8
Abstract
Introduction and Aim: Echis carinatus are small in size and are considered to be very biting invasive snakes. Snake venom is a type of snake saliva that is secreted through the salivary glands and stored in the venom sac. A problem in human societies, especially in rural areas, is snake bites, which ...
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Introduction and Aim: Echis carinatus are small in size and are considered to be very biting invasive snakes. Snake venom is a type of snake saliva that is secreted through the salivary glands and stored in the venom sac. A problem in human societies, especially in rural areas, is snake bites, which are not treated properly. The aim of this study is evaluation of toxicity of saliva of Echis carinatus sochureki on cells growth and the mechanisms involved. Methods: In this study, the effect of Echis carinatus sochureki saliva on human dermal fibroblasts (HDF) cells growth was determined by the inverted microscope, MTT assay, and Neutral red assay. The integrity of the cell membrane through LDH release was also measured. Results: The MTT assay and Neutral red assay showed a significant (p < /em>˂0.001) cytotoxic effect of Echis carinatus sochureki salvia on HDF cells growth after 24 hours treatment. Also, Echis carinatus sochureki venom caused a significant (p < /em>˂0.001) increase in LDH release. Various morphological abnormalities were observed in cells. Conclusion: The Echis carinatus sochureki saliva causes cytotoxic effects on HDF cells by the necrotic mechanism. The results of this study will be useful for future research in this field, as well as therapeutic methods for snake bites.
