tissue engineering and Regenerative medicine
Sona Zare; Rahim Ahmadi; DIBA SAMADI
Abstract
Background and Aim: Despite numerous studies on the biological properties and differentiability of umbilical cord-derived mesenchymal stem cells, these studies are still ongoing in order to achieve new findings. Therefore, the present study investigates the biological properties of umbilical cord-derived ...
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Background and Aim: Despite numerous studies on the biological properties and differentiability of umbilical cord-derived mesenchymal stem cells, these studies are still ongoing in order to achieve new findings. Therefore, the present study investigates the biological properties of umbilical cord-derived mesenchymal stem cells and their ability to differentiate into osteocyte and adipocyte.Materials and Methods: In this experimental laboratory study, 30 whole placenta specimens were prepared from the mothers under cesarean section and kept under standardized conditions. The mesenchymal cells were isolated by enzymatic method and their morphological characteristics were examined by microscopy and absorption spectroscopy and their biological properties, in particular expression of CD markers, were determined by flow cytometry. Finally, mesenchymal stem cells were cultured in specific media in order to differentiate into osteocyte and adipocyte. Data were analyzed using descriptive statistics.Results: Morphological and physical examinations by microscope and absorption spectroscopy as well as presenting of CD44, CD73, CD90, and CD105 markers and lacking CD34 and CD45 markers demonstrated the mesenchymal entity of stem cells. Mesenchymal stem cells successfully differentiated into osteocyte and adipocyte.Conclusion: Human cord-derived mesenchymal stem cells can differentiate into adult fat and bone cells. In this respect, the use of cord-derived mesenchymal cells could be of significant interest in cell therapy.
tissue engineering and Regenerative medicine
Akram Fadavi; Rahim Ahmadi; Sepideh Shahbaz Gahrouei; Maryam Hassan Nasab
Abstract
Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast ...
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Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast cells.Methods: During this experimental-laboratory study, the human neonatal foreskin was prepared and the fibroblast cells of the dermal layer were isolated and cultured. Cell viability was determined by flow cytometry and cell identity was determined using a Vimentin marker. After the preparation of chitosan hydrogel, fibroblast cells were loaded on it and the cytotoxic effect of hydrogel on cells was evaluated using the MTT assay. Data were analyzed using a t-test.Results: Cell counts showed that each dense T75 flask contained about 2 million cells. Isolated cells expressed the Vimentin marker at a high level. Fibroblasts were observed with good adhesion and uniform distribution on chitosan hydrogel. According to the results of the MTT test, the prepared hydrogel had no significant toxicity effect on fibroblast cells.Conclusion: The results of this study showed that chitosan scaffolding can be a suitable scaffold for loading fibroblast cells and transporting these cells for transplantation to damaged tissues.
tissue engineering and Regenerative medicine
Elaheh Roshanzadeh; Abbas Sahebghadam Lotfi; Sareh Arjmand
Volume 2, Issue 2 , October 2021, , Pages 8-20
Abstract
Introduction and Aim: Using 3D culture to increase cell density and creating a suitable bedrock for the growth and proliferation and differentiation of stem cells is one of the important methods for their application in tissue engineering. In this study, mesenchymal stem cells of adipose tissue because ...
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Introduction and Aim: Using 3D culture to increase cell density and creating a suitable bedrock for the growth and proliferation and differentiation of stem cells is one of the important methods for their application in tissue engineering. In this study, mesenchymal stem cells of adipose tissue because of ease of access and abundance have been used to differentiate into hepatocyte-like cells to investigate the effect of 3D cultures on gelatin/laminin 3D scaffolds.Methods: Mesenchymal stem cells were extracted from subcutaneous fat of the abdominal region of C57 mice by enzymatic digestion using collagenase enzyme. The extracted stem cells were confirmed by flow cytometry analysis and observation of the expression of specific stem cells markers such as CD105, CD44, and the absence of specific leukocyte (CD34) and hematopoietic (CD45). The non-toxicity of the scaffold was also investigated by the MTT method. Stem cells were cultured on a polystyrene surface and laminin and gelatin/laminin scaffold and treated with growth factors for 21 days in two stages. Cell differentiation was investigated by biochemical methods including examination of secretion indices such as urea and glycogen storage.Results: The expression of specific stem cells markers such as CD105, CD44, and the absence of CD34 and CD45 were proved. The ability to differentiate these cells into adipocytes and osteocytes has been proven.Conclusion: The results show the presence of a three-dimensional gelatin/laminin scaffold increases the adhesion, proliferation, and differentiation of fatty mesenchymal cells to hepatocyte-like cells.
