Alireza Iranbakhsh
Volume 1, Issue 1 , September 2020, , Pages 1-1
Cancer tissue and cell culture
Yeganeh Bahram Beygipour; Rahim Ahmadi; Hossein Zafari
Volume 2, Issue 1 , August 2021, , Pages 1-7
Abstract
Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was ...
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Introduction and Aim: Although several studies have been carried to investigate the effects of dandelion extracts on the viability of cervical cancer cells, the results on the effects of dandelion on cell viability, particularly in cervical cancer cells, are still challenging. The aim of this study was to investigate the cytotoxic effects of dandelion (Taraxacum officinale) extract on cervical cancer cells compared to non-cancerous cells.Methods: In this laboratory experimental study, HeLa cancer cell line and non-cancerous embryonic kidney cells (Hek293) were purchased from Pasteur Institute, Tehran, Iran. Cells were divided into control and treatment groups. In the treatment group, cells were exposed to 0.002, 0.02, 0.2, and 2 mg/ml of dandelion flower extract. MTT assay was used to evaluate cell viability. Data were analyzed using one-way analysis of varianceResults: Hela cancer cells viability was significantly reduced in the groups exposed to 0.2 and 2 mg/ml of extract compared to the control group (p<0.01 and P<0.001, respectively). None of the concentrations used had a significant effect on the viability of Hek293 cells.Conclusion: Dandelion flower extract in appropriate concentrations can reduce the viability of cervical cancer cells without side effects on healthy non-cancerous cells. The findings of this study support previous research indicating the anticancer effects of dandelion on cancer cells.
Cell culture and Tissue culture
Shahin Seyf; Rahim Ahmadi; Sara Najafi
Abstract
Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in ...
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Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in human red blood cells.Methods: In this experimental laboratory study, blood samples were obtained from healthy individuals, and blood samples were divided into the control group (treated with normal saline) and 2, 4, and 6 mg/kg of sulfasalazine receiving groups. Samples treated with 2 mg/kg of sulfasalazine were treated with 2, 4, and 6 mg/kg of Pissum sativum L and Aloe vera extract, and the erythrocyte membrane stability was calculated by standard methods. Data were analyzed using a one-way analysis of variance.Results: Sulfasalazine significantly reduced the stability of the erythrocyte membrane compared to the control group. Treatment of samples with concentrations of 4 and 6 mg/kg Pissum sativum L extract significantly increased membrane stability compared to the groups treated with sulfasalazine. Treatment with 2 and 4 mg/kg of Aloe vera extract did not increase membrane stability and a 6 mg/kg of Aloe vera extract non-significantly increased the membrane stability.Conclusion: Unlike Aloe vera, Pissum sativum L extract can increase the membrane stability of red blood cells in healthy people.
Plant Cell Culture and Tissue
Somayyeh Kheiri; Alameh Babajani
Abstract
Background and aim: This study was performed to identify the anatomical responses of lemon balm to zinc oxide nanoparticles (nZnO).Methods: This experimental laboratory study was done at the concentrations of 100 and 300 mg/L of nZnO.Results: The microscopic observation showed that the nZnO300 had the ...
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Background and aim: This study was performed to identify the anatomical responses of lemon balm to zinc oxide nanoparticles (nZnO).Methods: This experimental laboratory study was done at the concentrations of 100 and 300 mg/L of nZnO.Results: The microscopic observation showed that the nZnO300 had the largest diameter of the central cylinder and the diameter of xylem and phloem in the root compared to the control. The study of stem cross-section revealed that the highest stem diameter was observed in response to the nZnO100 group. The maximum thickness of collenchyma and the highest diameter of the central cylinder and the diameter of phloem and xylem were also recorded in the nZnO100 group. Comparing the leaf cross-sections indicated that the thickness of the upper and lower epidermis, the diameter of the xylem, and the thickness of the spongy parenchyma in the nZnO100 group were significantly increased relative to the control. A comparison of petiole cross-sections showed that the thickness of the upper and lower epidermis and the diameter of the central/lateral phloem and xylem in all nZnO-treated seedlings were significantly reduced compared to the control.Conclusion: This study provided anatomical evidence that indicates fundamental changes in the fate of cell differentiation and tissue formation in response to nZnO. Future research is needed to link physiological, molecular, and anatomical evidence to accurately understand the impact of nanoproducts on the entire life of plants, especially crops and medicines.
Zahra Oraghi Ardebili
Volume 1, Issue 3 , March 2021
Alireza Iranbakhsh
Volume 1, Issue 2 , December 2020
Toxicology
Abbas Zare MirakAbadi; Parvin Horrieh
Volume 1, Issue 1 , September 2020, , Pages 1-8
Abstract
Introduction and Aim: Echis carinatus are small in size and are considered to be very biting invasive snakes. Snake venom is a type of snake saliva that is secreted through the salivary glands and stored in the venom sac. A problem in human societies, especially in rural areas, is snake bites, which ...
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Introduction and Aim: Echis carinatus are small in size and are considered to be very biting invasive snakes. Snake venom is a type of snake saliva that is secreted through the salivary glands and stored in the venom sac. A problem in human societies, especially in rural areas, is snake bites, which are not treated properly. The aim of this study is evaluation of toxicity of saliva of Echis carinatus sochureki on cells growth and the mechanisms involved. Methods: In this study, the effect of Echis carinatus sochureki saliva on human dermal fibroblasts (HDF) cells growth was determined by the inverted microscope, MTT assay, and Neutral red assay. The integrity of the cell membrane through LDH release was also measured. Results: The MTT assay and Neutral red assay showed a significant (p < /em>˂0.001) cytotoxic effect of Echis carinatus sochureki salvia on HDF cells growth after 24 hours treatment. Also, Echis carinatus sochureki venom caused a significant (p < /em>˂0.001) increase in LDH release. Various morphological abnormalities were observed in cells. Conclusion: The Echis carinatus sochureki saliva causes cytotoxic effects on HDF cells by the necrotic mechanism. The results of this study will be useful for future research in this field, as well as therapeutic methods for snake bites.
Plant Cell Culture and Tissue
Nazila Bagheri; Bahram Maleki; Ali Ammarellou
Volume 1, Issue 2 , December 2020, , Pages 1-5
Abstract
Introduction and Aim: Myrtus communis L. is a perennial and aromatic herb with numerous medicinal properties. Myrtus communis L is a medicinal plant that is used in traditional medicine in many parts of the world. Propagation of this plant with cuttings or seeding is associated with particular difficulties. ...
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Introduction and Aim: Myrtus communis L. is a perennial and aromatic herb with numerous medicinal properties. Myrtus communis L is a medicinal plant that is used in traditional medicine in many parts of the world. Propagation of this plant with cuttings or seeding is associated with particular difficulties. The long seed dormancy period and the production of small and weak seedlings are the disadvantages of seed propagation. The optimization of in vitro tissue culture to facilitate the extraction of the component of this plant is important. Consequently, this study aimed to investigate the callus induction rate and callus initiation of Myrtus communis L. in different hormones. Methods: This experiment required a large number of germinated seeds of Myrtus communis L. in a Murashic-Scug (MS) medium. The seeds began to germinate after 20 days. Then, germinated seeds were divided into three studied explants: shoot explants, root explants, and leaf explants in MS medium containing 2,4-D. After 7 days, the callus production was recognized. The explants then were used to evaluate the regeneration rate of Myrtus communis L. in the MS medium containing growth regulators kinetin, BAP, TDZ, and combining the two hormones (BAP+TDZ) at four levels (0-1-2-3) mgl-1. Results: The results of this study showed the highest callus regeneration rate and callus growth rate were obtained from the leaf explants. Kentin hormone was the least effective hormone for regeneration of Myrtus communis L. and TDZ hormone had a greater effect than Kentin and BAP hormones. Additionally, the combination of 1.5 mgl-1 BAP with 1.5 mgl-1 TDZ, produced the highest callus regeneration in leaf explants. Conclusion: From the results of this study, it can be concluded that the regeneration of Myrtus communis L. is feasible through the application of leaf explants and a combination of BAP+TDZ hormones.
Cancer tissue and cell culture
Maryam Bikhof Torbati; Masoud Shaabanzadeh; Mahzad Motallebi
Volume 1, Issue 3 , March 2021, , Pages 1-7
Abstract
Introduction and Aim: Drug nanocarriers have been used extensively in cancer therapy due to their features like the ability to targeting drug transmission, increasing drug solubility, and reducing the drug cytotoxic effects on healthy tissues. The purpose of this study was to investigate the effects ...
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Introduction and Aim: Drug nanocarriers have been used extensively in cancer therapy due to their features like the ability to targeting drug transmission, increasing drug solubility, and reducing the drug cytotoxic effects on healthy tissues. The purpose of this study was to investigate the effects of Tamoxifen nanocapsules on the expression of Bax and Bak genes in MCF-7 cell lines. Methods: In this study, the nanocapsule structure was confirmed by FTIR spectroscopy and the effects of Tamoxifen nanocapsules on cell bioactivity were evaluated by MTT assay at concentrations of 10, 50, 100, and 200 µg/mL after 48 hours. Real-time PCR was used to analyze the expression of Bax and Bak genes and data were analyzed using SPSS (23.0) software. Results: According to the MTT assay, higher concentrations of Tamoxifen nanocapsules decreased cell bioactivity in a dose-dependent manner and the highest toxicity of nanocapsules was at the concentration of 200 µg/mL. The expression level of Bax and Bak genes in MCF-7 treated cells after 48 hours indicated the induction of apoptosis in cells. The results revealed a 1.8-fold increase in cytotoxicity of Tamoxifen nanocapsules compared to free Tamoxifen. Conclusion: The apoptosis induction as a result of increased expression of Bax and Bak genes and enhanced cytotoxicity, makes Tamoxifen nanocapsules a promising treatment for breast cancer therapy compared to free Tamoxifen.
Microbiology and Microbial media
Niloofar Seyfollahi; Simin Nabizadeh; Zahra Shafiei
Abstract
Introduction and Aim: In the second half of the last century, Pseudomonas aeruginosa is considered an important hospital pathogen, according to the US Centers for Disease Control. Pseudomonas aeruginosa is the fifth pathogen among hospital microorganisms, which comprises 10% of hospital-acquired infections, ...
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Introduction and Aim: In the second half of the last century, Pseudomonas aeruginosa is considered an important hospital pathogen, according to the US Centers for Disease Control. Pseudomonas aeruginosa is the fifth pathogen among hospital microorganisms, which comprises 10% of hospital-acquired infections, as the purslane plant has a variety of vitamins and minerals in fatty acids and as a fever antiseptic medication and it is used against spasm.Material and method: To evaluate the antimicrobial activity of aqueous and ethanolic extracts of Purslane, four standard microbial methods including mixed agar diffusion method, disk diffusion, the minimum inhibitory concentration of MIC, and minimum killer concentration of MBC using the microdilution method.Results: The results showed that silver nanoparticles obtained from purslane significantly reduced the growth of Pseudomonas, however, treatments of this bacterium with only purslane extract did not cause any effects on the growth of Pseudomonas.Conclusion: It can be said that the effect of silver nanoparticles from purslane was significantly higher than the extracts extracted from purslane. pH 10 combined with 2 mM nitrate salt yielded the most suitable dimensions for silver nanoparticles, and silver nanoparticles synthesized from purslane had far greater antibacterial effect than the extract extracted from purslane. So, this nanoparticle could be a good candidate to fight infection caused by Pseudomonas aeruginosa.
Plant Cell Culture and Tissue
Somayyeh Kheiri; Alameh Babajani
Abstract
Background and aim: This study was performed to identify the anatomical responses of lemon balm to selenium nanoparticles (nSe).Methods: This experimental laboratory study was done at the concentrations of 10 and 50 mg/L of nSe.Results: Plants were treated with nano-selenium (nSe; 0, 10 and 50 mg/l) ...
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Background and aim: This study was performed to identify the anatomical responses of lemon balm to selenium nanoparticles (nSe).Methods: This experimental laboratory study was done at the concentrations of 10 and 50 mg/L of nSe.Results: Plants were treated with nano-selenium (nSe; 0, 10 and 50 mg/l) and bulk selenium (BSe). The results of this study indicated that nano selenium had a positive and beneficial effect at a concentration of 10, but a concentration of 50 induced toxicity. The microscopic assessment of stem cross-sections showed that the diameter of the central cylinder was increased compared to the control. The highest increase was related to the seedlings exposed to the 50 and 10 mg/l. Stem thickness and diameter in the nSe-treated seedlings at 50 mg/l and bulk at 10 mg/l were increased compared to the control. Xylem diameter decreased in response to the supplements, the lowest belongs to the nSe50. Monitoring the leaf cross-sections showed that nSe10 increased the thickness of xylem diameter, palisade, and sponge parenchyma compared to the control. A comparison of petiole cross-sections indicated that the thickness of the epidermis and the diameter of the central and lateral xylem were changed. While 50 mg/l nSe declined these traits.Conclusion: Therefore, the results of this study indicate that the application of applied concentration-dependent selenium, in addition to inducing physiological and molecular changes, causes anatomical changes and tissue differentiation. Further research is needed in the future to determine the mechanism of these responses.
Cell culture and Tissue culture
Sona Zare; MohammadAli Nilforoushzade; Rahim Ahmadi; Zahra Esmaeili
Volume 2, Issue 1 , August 2021, , Pages 8-14
Abstract
Introduction and Aim: Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate ...
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Introduction and Aim: Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate and evaluate the cell population of human adipose stromal vascular fraction with the main aim of investigating the presence of stem cells in human adipose stromal vascular fraction.Methods: In this laboratory-experimental study adipose tissues were obtained from 10 healthy individuals (30 to 58 years) and maintained in standard condition. After enzymatic isolation, the viability of stromal vascular fraction cells and surface antigens was evaluated by flow cytometry.Results: The results of this study showed that adipose stromal vascular fraction cells had high viability (> 98%) and were positive for CD16, CD34, CD73, CD29, CD105, CD31, and CD45 markers and negative for CD3, CD19, and CD38 markers.Conclusion: The findings of this study indicate the presence of adipose-derived stem cells, endothelial cells, peri-adipocytes, and macrophages. According to this, this fraction has potential in the clinical field of cell therapy.
Virology and Viral transport medium
Azadeh Hekmat
Abstract
Humans have been battling viruses since before our species had even evolved into its modern form. some viruses are equally deadly, and some that are even deadlier. Accurate and early detection of viruses is often crucial for clinical diagnosis and therapy. Aptamers are the artificial single-stranded ...
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Humans have been battling viruses since before our species had even evolved into its modern form. some viruses are equally deadly, and some that are even deadlier. Accurate and early detection of viruses is often crucial for clinical diagnosis and therapy. Aptamers are the artificial single-stranded DNA, RNA sequences, or peptides that can bind to certain targets with tremendously high specificity. A number of their unique features make them a more effective choice than antibodies. Aptamers are typically generated through Systematic Evolution of Ligands by Exponential enrichment (SELEX) and screened and selected via in vitro process from a library, making it possible to attach to any target molecules. In this review, aptamers were briefly defined and their applications in the rapid detection and diagnosis of some deadly viruses (HIV, Ebola, IVA, IVB, HPV, SARS-CoV, MERS-CoV, and COVID-19) were described.
Cancer tissue and cell culture
Zainab Kamrani; Masoumeh Heshmati; Sadegh Babashah
Volume 1, Issue 3 , March 2021, , Pages 8-15
Abstract
Introduction and Aim: Silymarin, extracted from the milk thistle, is rich in flavolignans such as silybinin, silidianin, and silycristine. Several studies have been done on its anticancer effect. This study aimed to evaluate the effects of cytotoxicity and induction of silymarin-induced apoptosis on ...
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Introduction and Aim: Silymarin, extracted from the milk thistle, is rich in flavolignans such as silybinin, silidianin, and silycristine. Several studies have been done on its anticancer effect. This study aimed to evaluate the effects of cytotoxicity and induction of silymarin-induced apoptosis on colon cancer SW480 and normal HEK-293 cell lines. Methods: In the present study, the effect of cytotoxicity and apoptosis induction by silymarin on colon cancer SW480 and Normal HEK-293 cell lines were investigated by different methods such as trypan blue staining, MTT assay, Annexin V/PI staining, and evaluating the expression level of BAX and BCL2 Genes by q-PCR technique. Results: The results of the trypan blue staining and MTT assay indicated the cytotoxicity effects of silymarin in SW480 cells in a time-dependent manner. The IC50 was also evaluated. However, no cytotoxic effects were observed in HEK-293 cells after treatment with silymarin. Also, an increase of apoptosis in SW480 cells that were treated with 50 and 100 µg/mL silymarin was observed. An increase in BAX/BCL2 gene expression level was also observed in SW480 cells compared to HEK-293 cells. Conclusion: According to the results of the present study, silymarin can induce toxicity and cell apoptosis in the colon cancer cell line without causing cytotoxicity in the normal cell line. So silymarin can be suggested for effective treatment of colon cancer.
Medical Genetics
Saeed Zaker Bostanabad; Soheila Darmanlo; Somayeh Darmanloo
Volume 1, Issue 1 , September 2020, , Pages 9-15
Abstract
Introduction and Aim: According to the WHO definition, recurrent miscarriage is a consecutive pregnancy loss before weeks 22. Different factors involved in recurrent abortion as a multifactorial disease. Genetic factors are one of the important factors associated with recurrent abortion. VEGFA (Vascular ...
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Introduction and Aim: According to the WHO definition, recurrent miscarriage is a consecutive pregnancy loss before weeks 22. Different factors involved in recurrent abortion as a multifactorial disease. Genetic factors are one of the important factors associated with recurrent abortion. VEGFA (Vascular Endothelial Growth Factor A) is one of the important gene in angiogenesis. MTHFD1 (Methylenetetrahydrofolate Dehydrogenase, Cyclohydrolase, And Formyltetrahydrofolate Synthetase 1) is a protein-coding gene that metabolism folate. The goal of this article is to assess the association between single nucleotide polymorphisms of VEGFA and MTHFD1 and the recurrent miscarriage in Iranian women.Material and method: The study group was 50 women with recurrent miscarriages and the control groups consisted of 10 women with at least two successful pregnancies and no miscarriages. The DNA extracted from leucocytes cells. PCR was used to find the association between VEGFA and MTHFD1 genes and recurrent miscarriages. Analyzing performed with FinchTV software after data sequencing.Result: Our results showed that the polymorphism frequency of CC and CT in rs2236225 VEGFA were 86% and 14% in women with recurrent miscarriages and 90% and 10% in the control group. the polymorphism frequency of GG and GA in rs3025039MTHFD1 were 82% and 18% in women with recurrent miscarriages and no polymorphism was detected in the control group.Conclusion: These results showed that there is an association between single nucleotide polymorphisms of VEGFA and MTHFD1 and the recurrent miscarriage in Iranian women. Furthermore, MTHFD1 polymorphism could be a molecular marker in the diagnosis of recurrent miscarriage.
Cell culture and Tissue culture
sasan Kalantari; Tahereh Naji; Rahim Ahmadi
Abstract
Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar ...
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Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar rats.Methods: In this study, 20 two-month-old adult male Wistar albino rats weighing approximately 200-150 g were studied in two groups: the control group (healthy) and the busulfan group. After this period, the testicular tissue of the mice was isolated and evaluated for the expression of Nrf-2 and Keap-1 genes, as well as changes in testosterone, FSH, and LH. Data were analyzed using SPSS software and a one-way ANOVA statistical test.Results: The statistical analysis of the results showed that the expression of Nrf-2 and Keap-1 genes in the treatment group (busulfan) decreased compared to the control group, but this decrease was not statistically significant (0.2951 and P = 0.3528). LH and testosterone levels were not significantly different between the control and treated groups (0.1327 and P = 0.0809) but FSH levels were significant between the two groups (P = 0.0189).Conclusion: The results of this study showed that busulfan can affect the level of FSH, which is effective in spermatogenesis, by causing changes in the expression of keap1 and Nrf-2 genes and causing infertility. Therefore, these genetic and hormonal factors can be used to predict infertility.
tissue engineering and Regenerative medicine
Sona Zare; Rahim Ahmadi; DIBA SAMADI
Abstract
Background and Aim: Despite numerous studies on the biological properties and differentiability of umbilical cord-derived mesenchymal stem cells, these studies are still ongoing in order to achieve new findings. Therefore, the present study investigates the biological properties of umbilical cord-derived ...
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Background and Aim: Despite numerous studies on the biological properties and differentiability of umbilical cord-derived mesenchymal stem cells, these studies are still ongoing in order to achieve new findings. Therefore, the present study investigates the biological properties of umbilical cord-derived mesenchymal stem cells and their ability to differentiate into osteocyte and adipocyte.Materials and Methods: In this experimental laboratory study, 30 whole placenta specimens were prepared from the mothers under cesarean section and kept under standardized conditions. The mesenchymal cells were isolated by enzymatic method and their morphological characteristics were examined by microscopy and absorption spectroscopy and their biological properties, in particular expression of CD markers, were determined by flow cytometry. Finally, mesenchymal stem cells were cultured in specific media in order to differentiate into osteocyte and adipocyte. Data were analyzed using descriptive statistics.Results: Morphological and physical examinations by microscope and absorption spectroscopy as well as presenting of CD44, CD73, CD90, and CD105 markers and lacking CD34 and CD45 markers demonstrated the mesenchymal entity of stem cells. Mesenchymal stem cells successfully differentiated into osteocyte and adipocyte.Conclusion: Human cord-derived mesenchymal stem cells can differentiate into adult fat and bone cells. In this respect, the use of cord-derived mesenchymal cells could be of significant interest in cell therapy.
Animal cell and culture
Zeynab Akbarkashani; Azadeh Hekmat; Seyed Mohammad Atyabi
Volume 2, Issue 1 , August 2021, , Pages 15-20
Abstract
Introduction and Aim: Human chorionic gonadotropin (hCG) is a glycoprotein produced during pregnancy by trophoblast and placental syncytiotrophoblast cells. The produced hCG hormone affects the corpus luteum and prevents it from being destroyed. Studies show that diamond nanoparticles (NDs) interact ...
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Introduction and Aim: Human chorionic gonadotropin (hCG) is a glycoprotein produced during pregnancy by trophoblast and placental syncytiotrophoblast cells. The produced hCG hormone affects the corpus luteum and prevents it from being destroyed. Studies show that diamond nanoparticles (NDs) interact with biological systems such as proteins, enzymes, and antibodies. In other words, NDs are capable of being absorbed by biomolecules and can also be used as a carrier for the transport of biologically active substances. This study aimed to investigate the effect of NDs on hCG structure and on hCG level in Wistar rats.Methods: The effect of 10 nm NDs on the structure of hCG was investigated using spectroscopic techniques. Rats were randomly divided into five groups: Control, sham, and experimental groups (received NDs solution at doses of 25, 75, and 100 mg/ml). Injections were carried out twice a week. Then, serum biochemical parameters were calculated. Data were analyzed utilizing one-way ANOVA followed by Tukey's multiple comparisons test.Results: The results showed that NDs could form a complex with hCG. The zeta potential and DLS results showed that NDs cause a decrease in the surface charge and size of hCG. Administration of NDs in concentrations of 100 mg/ml significantly decreased hCG levels compared with that of control samples (P<0.05),Conclusion: Based on the investigation NDs could change the tertiary structure of hCG. NDs had toxic effects on hCG function. More studies to explore the toxic effects of NDs in various doses and times of exposure seem to be necessary.
Cancer tissue and cell culture
Ali Asghar Azizi Jirabadi; Seyed Ataollah Sadat Shandiz; Maryam Abbasi
Volume 1, Issue 2 , December 2020, , Pages 15-20
Abstract
Introduction and Aim: Breast Cancer is the most prevalent type of tumor and is also have second major death among women. The aim of the current study was to investigate the anticancer properties of Phormidiumm Animale cyanobacterium extract using MTT assay and through modulation of p53 gene expression ...
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Introduction and Aim: Breast Cancer is the most prevalent type of tumor and is also have second major death among women. The aim of the current study was to investigate the anticancer properties of Phormidiumm Animale cyanobacterium extract using MTT assay and through modulation of p53 gene expression in breast cancer cells. Methods: In the current study, the breast cancer MCF-7 and normal HEK293 cell lines were treated with various concentrations of P. animale extract ranging 50, 100, 200 mg/mL for 24 hours. Cell viability was measured using MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay against cell lines. The gene expressions of p53 compared with GAPDH were measured using the real-time PCR method. Results: The MTT results showed that P. animale significantly decreased the viability of cancer cells compared to normal cells in dose-dependent mode. Moreover, the mRNA levels of p53 were significantly increased from 3.34 in MCF-7 cells to 5.15 and 9.11 fold, respectively, following treatment with 50, 100, and 200 mg/mL P. animale extract. Conclusion: The data suggest that P. animale extract can induce cytotoxicity and might modulate apoptosis by up-regulating p53 gene expression in human breast cancer MCF-7 cell line.
Medical Genetics
Ani Dejakam; Ani Dejakam; Azadeh Hekmat
Volume 1, Issue 1 , September 2020, , Pages 16-25
Abstract
Introduction and Aim: Opium addiction is one of the most prevalent addiction in Iranian society. Throughout the last two decades, congress 60; a nongovernmental organization; has been operated a taper off treatment of opium associated with a package of psychological treatment group classes. Although ...
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Introduction and Aim: Opium addiction is one of the most prevalent addiction in Iranian society. Throughout the last two decades, congress 60; a nongovernmental organization; has been operated a taper off treatment of opium associated with a package of psychological treatment group classes. Although the effectiveness of the taper off method in opium addiction has been verified, molecular mechanisms involved in this treatment have not explained. BDNF gene is a brain-derived neurotrophic factor that is associated with numerous molecular mechanisms of the brain including memory. Methods: In this research, peripheral blood samples from 21 patients were collected from Congress 60. RNA was extracted from each sample, reverse transcribed and amplified via RT-PCR technique, utilizing specific primers for BDNF. The production of BDNF protein was also analyzed employing the western blotting technique. Results: Our results exhibited considerable down expression of BDNF in addict persons vs. non-psychiatric persons (p < /em><0.01). Furthermore, BDNF expression levels in addicts increased significantly after the therapy period. Findings showed the effect of opium abuse and taper off treatment on the expression of BDNF. Conclusion: The present study could help to a better understanding of molecular and neurological mechanisms of opium and taper off treatment. Additionally, BDNF level detection could be a potential marker for screening the effectiveness of various types of addiction treatment.
Medical Genetics
Flora Forouzesh; Pantea Hajimirza Shafiesoltani; Mahsa Ghiaghi; Mahdi Shabani
Volume 1, Issue 3 , March 2021, , Pages 16-24
Abstract
Introduction and Aim: Colorectal cancer is one of the most common cancers. Epigenetic change has been considered by many scientists as a therapeutic target. Hyper acetylation of chromatin components by sodium butyrate can alter gene regulation. This study aims to investigate the effects of sodium butyrate ...
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Introduction and Aim: Colorectal cancer is one of the most common cancers. Epigenetic change has been considered by many scientists as a therapeutic target. Hyper acetylation of chromatin components by sodium butyrate can alter gene regulation. This study aims to investigate the effects of sodium butyrate on Bax and Bcl-2 gene expression. Methods: Caco-2 cell line was treated with different concentrations of sodium butyrate (25 mM to 150 mM) based on IC50 concentration in two time periods of 24 hours and 48 hours. Bax and Bcl-2 gene expression were measured by qReal-Time PCR technique and Bcl2/Bax ratio was evaluated. Results: The results showed that sodium butyrate increased the expression of Bax gene and decreased the expression of Bcl-2 gene in treated cells compared to the control group, which was statistically significant (p < /em> <0.05), and 25 mM was selected as the most effective dose after 48 hours of treatment. Also, the Bcl-2/Bax ratio at the same concentration showed a significant decrease Conclusion: Sodium butyrate induces apoptosis in cancer cells by reducing the expression ratio of Bcl-2/Bax. It can be used as a therapeutic target but needs further investigation.
Cancer tissue and cell culture
Mahdi Azadmanesh; Tahereh Naji; Abdolreza Mohammadnia
Abstract
Introduction and aim: Genes play a very important role in the development of cancer. This study aimed to evaluate three very important genes (C-fos, C-jun, and P53) in Wistar mice's stomach tissue cancer cells designed by dimethylhydrazine.Methods: In this study, forty male Wistar mice, 57 weeks old, ...
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Introduction and aim: Genes play a very important role in the development of cancer. This study aimed to evaluate three very important genes (C-fos, C-jun, and P53) in Wistar mice's stomach tissue cancer cells designed by dimethylhydrazine.Methods: In this study, forty male Wistar mice, 57 weeks old, were evaluated and divided into four groups of Ten. In the control group, the second group received Cinnamaldehyde-free carcinogens, the third group was healthy with the Cinnamaldehyde, and the fourth group received carcinogens with the Cinnamaldehyde at the same time. Then, the gastric tissue of mice was isolated and evaluated for the expression of C-fos, C-Jun, and P53 genes. The data were analyzed with One-way ANOVA using SPSS.Results: Statistical analysis of the results showed that the expression of C-fos, C-Jun, and P53 genes in the carcinogen receptor with the Cinnamaldehyde was increased compared to the control group. This increase was statistically significant for two genes C-fos (P = 0.0146) and P53 (P = 0.0212) and for the C-Jun gene (P = 0.01604) this increase was not significant.Conclusion: The results of this study showed that Cinnamaldehyde can alter the expression of important genes C-fos, C-Jun, and P53 that are effective in causing gastric cancer.
Animal cell and culture
Talie Esmaeeli; Ramazan Khanbabaee; Azadeh Hekmat
Abstract
Background and Aim: Noise pollution is one of the hazardous factors in the environment. One of the major sources of this sort of pollution is the noise produced by urban transportation, mostly subway and business centers. In this study, the noise pollution effects on the histopathological changes in ...
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Background and Aim: Noise pollution is one of the hazardous factors in the environment. One of the major sources of this sort of pollution is the noise produced by urban transportation, mostly subway and business centers. In this study, the noise pollution effects on the histopathological changes in the liver and kidney of Wistar male rats were investigated.Materials and Methods: Rats were arranged in 3 groups (control, and experimental, n=6). The control group was kept in the standard situation and the experimental groups were kept in Tehran grand bazaar and around sadeghiyeh metro station (west rose street). After eight weeks all animals were euthanized via cervical dislocation and tissue samples stained with hematoxylin and eosin for histopathology evaluation. Results: In the samples of all the rats located around Sadeghiyeh subway and Tehran's grand bazaar, there is no difference in kidney volume between the control and experimental groups. Nonetheless the destruction of hepatocytes and cell degeneration around the central vein in liver tissue were observed in group placed around Sadeghiyeh subway.Conclusion: The results of this study showed that noise pollution in the two areas of Tehran's grand bazaar and Sadeghiyeh subway causes changes in liver tissue. Consequently, control and correction measures to reduce noise in these areas seem necessary.
Cancer tissue and cell culture
Hossein Gravand; Jafar Kazemian; Masoumeh Heshmati
Volume 2, Issue 1 , August 2021, , Pages 21-27
Abstract
Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis ...
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Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis induction of 7-hydroxycycoumarin were investigated on the SW480 colon cancer cell line.Methods: anti-proliferative effect of 7-hydroxycoumarin was investigated using Trypan blue and MTT assay at different concentrations on SW480 cell line. Additionally, apoptosis induction was examined by flow cytometry and real-time PCR.Results: The results show a decrease in viability. IC50 concentrations were observed at the concentrations of 164.2, 80.48, and 41.97 μg/ml for 24, 48, and 72 h, respectively. Of note, IC50 concentrations were not observed in the trypan blue assay. As well, there was a 2.4-fold increase in apoptosis (P<0.001) using Annexin / PI kit by flow cytometry. as well as the increased expression of BAX/BCL2 ratio of about 13.69, which was observed at 100 μg/ml compared to the control.Discussion: The results indicate that 7-hydroxycoumarin could inhibit cell proliferation and induce apoptosis in the SW480 cell line. Therefore, 7-hydroxycycoumarin can be considered as an anti-cancer agent in the complementary treatment of colon cancer.
Allergy and Algal culture media
Ali Naseri; Mostafa Noroozi
Volume 1, Issue 2 , December 2020, , Pages 21-26
Abstract
Introduction and Aim: Diatoms are one of the most important branches of algae which are classified as pinnate and centric groups. Taleghan River is one of the main tributaries of Sepid Roud in Iran, which has 180 km in length. This study was complementary to the previous diatom projects in Taleghan River ...
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Introduction and Aim: Diatoms are one of the most important branches of algae which are classified as pinnate and centric groups. Taleghan River is one of the main tributaries of Sepid Roud in Iran, which has 180 km in length. This study was complementary to the previous diatom projects in Taleghan River and introducing the new record for this area. Methods: This study was carried out from Shahrivar 1398 to Shahrivar 1399 for one year and samples were collected seasonally from two upstream stations. 60 ml of precipitates was collected from 1 cm depth of the River using a syringe and fixed in 4% Formalin. The permanent slides were provided based on the standard protocol and the images were captured with a photomicroscope. Results: During the sampling from two stations upstream of Taleghan Dam, Pinnularia grunowii Krammer was identified as a new record for the diatom flora of Iran. The most important morphological characters of this taxon are the valve outline treeudulate, non-curved striae, and it's size which make it different from the other similar taxa such as; Pinnularia subanglica Petersen, Pinnularia biceps Gregory, Pinnularia lundii var. linearis Krammer, and Pinnularia angusta var. angusta (Cleve) Krammer. Conclusion: It is necessary to investigate in other ecosystems of Iran to understand the ecology and distribution of the Pinnularia grunowii Krammer.
