Original Article
Cell culture and Tissue culture
mahdi farhadi mahalli; pejman mortazavi; Saeed Motesaddi Zarandi; akram eidi
Abstract
Introduction and aim: In this study, we have investigated the role of gaseous pollutants and PM2.5 on changes in some blood parameters and inflammatory tissue factors in a rat model in treatment period of six months.Methods: A total of 24 Wistar rats were randomly divided into 3 groups: receiving PM2.5 ...
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Introduction and aim: In this study, we have investigated the role of gaseous pollutants and PM2.5 on changes in some blood parameters and inflammatory tissue factors in a rat model in treatment period of six months.Methods: A total of 24 Wistar rats were randomly divided into 3 groups: receiving PM2.5 + gaseous pollutants (6 months), gaseous pollutants (6 months) and clean air intake control (6 months).Results: The results showed that the groups treated with PM2.5 + gaseous pollutants showed a significant reduction in the RBC, WBC, HB and PLT count compared to the control group. The results also showed that the level of both interleukin 6 and TNF-α in lung tissue and interleukin 10 were significantly increased and decreased in the PM2.5 + gaseous pollutant group at 6-month period compared to the control group, respectively.Conclusion: Air pollution PM2.5 as one of the most important causes of disease and mortality worldwide can cause changes in blood parameters such as blood cells and change (increase or decrease) and some cytokines such as interleukin 6, 10 and TNF- α in lung tissue. These inflammatory cytokines may cause the formation of free radicals and their destructive effects in the lung.
Original Article
Cell culture and Tissue culture
Mohamad Mahdi Ramezani; Ali Asghar Razmaraei Iranagh; Reaza Aghaei; Nasser Razmaraii
Abstract
Introduction and aim: Cultured poultry cells can provide a valuable resource for various researchers in the field of pharmaceutical studies, the culture of poultry and human viruses, as well as the production of viral vaccines. Avian cell culture requires an optimal basic medium and currently, there ...
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Introduction and aim: Cultured poultry cells can provide a valuable resource for various researchers in the field of pharmaceutical studies, the culture of poultry and human viruses, as well as the production of viral vaccines. Avian cell culture requires an optimal basic medium and currently, there are limited options for this basic medium. This means that there is still room to improve an optimal basal medium for avian cell culture.Methods: In this study, fibroblast cells were isolated from SPF chickens and the growth and proliferation of fibroblast cells were done in three culture mediums: DMEM with high glucose, DMEM with low glucose, and RMI1640 culture medium enriched with different concentrations of fetal calf serum (FBS), were evaluated.Results: The results of this study showed that there is a significant difference in the growth and proliferation of fibroblasts cultured in DMEM culture medium with high glucose enriched with 10% FBS compared to DMEM culture medium with low glucose and RMI1640 culture medium enriched with 10% FBS. There were (p<0.05). However, no significant difference was observed in the doubling time of these cells in three culture media.Conclusion: These results show that it is easy to isolate chicken embryo fibroblast cells in the laboratory and provide the necessary conditions for the growth and proliferation of these cells by using a DMEM culture medium with high glucose enriched with 10% FBS. The results of this study can be used by researchers in various pharmaceutical and virological research and the production of poultry viral vaccines.
Original Article
tissue engineering and Regenerative medicine
sona zare; masoumeh Aalipour; hsdis rostami motamed
Abstract
Background and Aim: Many studies have shown that the amniotic membrane can be used to prepare a suitable acellular sheet. At the same time, the quality of acellular sheets prepared by different methods is different. Based on this, the present study aims to obtain a suitable scaffold prepared from the ...
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Background and Aim: Many studies have shown that the amniotic membrane can be used to prepare a suitable acellular sheet. At the same time, the quality of acellular sheets prepared by different methods is different. Based on this, the present study aims to obtain a suitable scaffold prepared from the amniotic membrane, investigate the characteristics and evaluate the acellular sheet derived from the amniotic membrane. Methods: During this experimental laboratory study, the complete sample of placenta was isolated from mothers who underwent cesarean section. The amnion membrane was treated with PBS solution and 0.03% (w/v) sodium dodecyl sulfate and Tris-buffered saline (TBS) solution and decellularized.Results: The results of examination with electron microscope showed that there is no cell content in the prepared acellular sheet.Conclusion: Overall, the results of this research showed that the acellular sheet prepared from human amniotic membrane has good quality as a biological scaffold.
Original Article
Cancer tissue and cell culture
Kimia Farokhi Bahar; Azadeh Hekmat
Abstract
Introduction and aim: Palm oil (PO) is the second largest vegetable oil in the world and contains large quantities of phytochemicals, for example, vitamin E, carotenoids, and phytosterols. This study aimed to investigate the effect of Palm oil on the cellular growth of K652 blood cancer, as a model of ...
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Introduction and aim: Palm oil (PO) is the second largest vegetable oil in the world and contains large quantities of phytochemicals, for example, vitamin E, carotenoids, and phytosterols. This study aimed to investigate the effect of Palm oil on the cellular growth of K652 blood cancer, as a model of cancer cells.Methods: In this study, K562 leukemia cells were treated with concentrations of 5, 25, 50, 75, and 100 µg per mL of PO for 24 and 48 hours. MTT assay, NAC assay, and microscopic observations were used to evaluate cell changes and cell death/survival.Results: PO at concentrations above 5 μg per ml induced significant cell death. Microscopic observations also confirmed cell death in cells that were treated with PO. Also, the rate of inhibition of cell growth of PO after 24 hours was more than 48 hours. After NAC treatment, 81% cell survival was observed (P <0.001).Conclusion: According to the results of this study, PO has a great inhibitory effect on K562 leukemia cells and could be a good candidate for cancer therapy.
Editorial
Volume 3, Issue 2 , November 2023
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