نوع مقاله : مروری

نویسنده

گروه زیست شناسی، دانشکده علوم، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

چکیده

سیانوباکتری ها، موجودات میکروسکوپی دارای ویژگی های مشابه با پروکاریوت ها و یوکاریوت ها هستند که امروزه در تحقیقات بیوتکنولوژی بسیار مطرح هستند و قادرند طیف وسیعی از ترکیبات آلی بسیار ارزشمند را تولید نمایند. با این حال هنوز گروه کثیری از سویه های سیانوباکتری ها در ایران ناشناخته باقی مانده اند، گروهی که به آب های شیرین (برکه و آبگیر یا چشمه و نهر) اختصاص دارند و گروهی که به زیستگاه های خشکی مربوط هستند، این امر در بسیاری از موارد به دلیل این است که روش های کشت و پرورش اختصاصی سویه های سیانوباکتری ها به درستی شناخته نشده است. در واقع کشت سویه های سیانوباکتری های خالص شده و آکزنیک، فاقد هر گونه باکتری و پاتوژن دیگر، امکان انجام بسیاری از فعالیت های پژوهشی را در زمینه های مختلف ژنتیکی و مولکولی فراهم خواهد آورد. در این مقاله با هدف آشنایی با محیط کشت های مختلف برای انواع سویه های سیانوباکتری ها، روش درست و دقیق جداسازی، کشت و خالص سازی سیانوباکتری های آبزی و خاکزی توضیح داده خواهد شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Culturing of Aquatic and terrestrial cyanobacteria

نویسنده [English]

  • Bahareh Nowruzi

Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Cyanobacteria, microscopic organisms have similar characteristics to prokaryotes and eukaryotes, which are very popular in biotechnology research today and are able to produce a wide range of highly valuable organic compounds. However, many strains of cyanobacteria still remain unknown in Iran, a group that is dedicated to freshwater (ponds or springs and streams) and a group that is related to terrestrial habitats. This is in many cases due to the fact that the specific cultivation methods of cyanobacterial strains are not well known. In fact, culturing the strains of axenic cyanobacteria, free of any bacteria or other pathogens, will allow many research activities to be performed in various genetic and molecular fields. In this article, in order to get acquainted with different media cultures for different types of cyanobacterial strains, the correct and accurate method of isolation, cultivation and purification of aquatic and terrestrial, cyanobacteria will be explained.
Key words: Cyanobacteria, Culturing, Aquatic, Terrestrial.

کلیدواژه‌ها [English]

  • Cyanobacteria
  • Culturing
  • Aquatic
  • Terrestrial
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