Document Type : Review


Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran


Cyanobacteria, microscopic organisms have similar characteristics to prokaryotes and eukaryotes, which are very popular in biotechnology research today and are able to produce a wide range of highly valuable organic compounds. However, many strains of cyanobacteria still remain unknown in Iran, a group that is dedicated to freshwater (ponds or springs and streams) and a group that is related to terrestrial habitats. This is in many cases due to the fact that the specific cultivation methods of cyanobacterial strains are not well known. In fact, culturing the strains of axenic cyanobacteria, free of any bacteria or other pathogens, will allow many research activities to be performed in various genetic and molecular fields. In this article, in order to get acquainted with different media cultures for different types of cyanobacterial strains, the correct and accurate method of isolation, cultivation and purification of aquatic and terrestrial, cyanobacteria will be explained.
Key words: Cyanobacteria, Culturing, Aquatic, Terrestrial.


Main Subjects

Ahmadi Moghadam, A. and Nourozi, B. 2008. A new report of n fixation by two species of cyanobacteria.
Berg, K. 2009. Heterotrophic bacteria associated with cyanobacteria in recreational and drinking water.
Hosseini, N., Akhavan, A. and Nowruzi, B. 2019. Detection and relation of polyketide synthase (pkss) genes with antimicrobial activity in terrestrial cyanobacteria of lavasan. Iranian Journal of Medical Microbiology, 12(6): 419-431.
Komarek, J. 2006. Cyanobacterial taxonomy: Current problems and prospects for the integration of traditional and molecular approaches. Algae, 21(4): 349-375.
Komárek, J. 2010. Modern taxonomic revision of planktic nostocacean cyanobacteria: A short review of genera. Hydrobiologia, 639(1): 231-243.
Komárek, J. 2010. Recent changes (2008) in cyanobacteria taxonomy based on a combination of molecular background with phenotype and ecological consequences (genus and species concept). Hydrobiologia, 639(1): 245-259.
Komárek, J. and Anagnostidis, K. 2005. Bd. 19/2: Cyanoprokaryota: Teil 2: Oscillatoriales. Elsevier, München.
Komárek, J., Kaštovský, J., Mareš, J. and Johansen, J.R. 2014. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. Preslia, 86(4): 295-335.
Kotai, J. 1972. Instructions for preparation of modified nutrient solution z8 for algae. Norwegian Institute for Water Research, Oslo, 11(69): 5.
Liu, L., Jokela, J., Wahlsten, M., Nowruzi, B., Permi, P., Zhang, Y.Z., Xhaard, H., Fewer, D.P. and Sivonen, K. 2014. Nostosins, trypsin inhibitors isolated from the terrestrial cyanobacterium nostoc sp. Strain fsn. Journal of natural products, 77(8): 1784-1790.
Niva, A. 1976. Estimation of algal growth potential. Norwegian Inst Water Res, 11(82): 2-25.
Nourozi, B. and Ahmadi Moghadam, A. 2006. Determination of generation time and growth curve of nostoc muscorum and nostoc ellipsosporum by measuring of chlorophyll in modified allen's medium.
Nourozi, B. and Ahmadi Moghadam, A. 2007. New records of relationship between soil macro elements and the distribution of heterocystous cyanobacteria in paddy fields, wheat fields and woodland in golestan province.
Nourozi, B. and Ahmadi Moghadam, A. 2007. New records on morphological variation of nostoc muscorum and nostoc ellipsosporum on benecks, and modified allen's, media during their life cycle.
Nowruzi, B. and Ahmadimoghadam, A. 2006. Two new records of heterocystus cyanobacteria (nostocaceae) from paddy fields of golestan province. Iran. Journ. Bot, 11(2): 170-173.
Nowruzi, B., AKHAVAN, S.A. and Soltani, S.G.S. 2019. Genetic analysis of nonribosomal peptide synthesis genes (nrpss) in natural product biosynthesis of the cyanobacterial strains of lavasan lake.
Nowruzi, B. and Blanco, S. 2019. In silico identification and evolutionary analysis of candidate genes involved in the biosynthesis methylproline genes in cyanobacteria strains of iran. Phytochemistry Letters, 29: 199-211.
Nowruzi, B., Blanco, S. and Nejadsattari, T. 2018. Chemical and molecular evidences for the poisoning of a duck by anatoxin-a, nodularin and cryptophycin at the coast of lake shoormast (mazandaran province, iran). Int J Algae, 20(4).
Nowruzi, B., Fahimi, H. and Ordodari, N. 2018. Molecular phylogenetic and morphometric evaluation of calothrix sp. N42 and scytonema sp. N11. Rostaniha, 18(2): 210-221.
Nowruzi, B., Fahimi, H., Ordodari, N. and Assareh, R. 2017. Genetic analysis of polyketide synthase and peptide synthase genes of‎ cyanobacteria as a mining tool for new pharmaceutical compounds. Journal of Pharmaceutical & Health Sciences, 5(2): 139-150.
Nowruzi, B., Haghighat, S., Fahimi, H. and Mohammadi, E. 2018. Nostoc cyanobacteria species: A new and rich source of novel bioactive compounds with pharmaceutical potential. Journal of Pharmaceutical Health Services Research, 9(1): 5-12.
Nowruzi, B., Khavari-Nejad, R.-A., Nejadsattari, T., Sivonen, K. and Fewer, D. 2017. A proposal for the unification of two cyanobacterial strains of nostoc as the same species. Rostaniha, 17(2): 161-172.
Nowruzi, B., Khavari-Nejad, R.-A., Sivonen, K., Kazemi, B., Najafi, F. and Nejadsattari, T. 2012. Identification and toxigenic potential of a nostoc sp. Algae, 27(4): 303.
Nowruzi, B., Khavari-Nejad, R., Sivonen, K., Kazemi, B., Najafi, F. and Nejadsattari, T. 2013. Optimization of cultivation conditions to maximize extracellular investments of two nostoc strains. Algological Studies, 142(1): 63-76.
Nowruzi, B., Khavari-Nejad, R.A., Sivonen, K., Kazemi, B., Najafi, F. and Nejadsattari, T. 2013. Identification and toxigenic potential of a cyanobacterial strain (stigomena sp.). Progress in Biological Sciences, 3(1): 79-85.
Nowruzi, B., Sarvari, G. and Blanco, S. 2020. Applications of cyanobacteria in biomedicine. In: Handb. Algal sci. Microbiol. Technol. Med. Elsevier Amsterdam.
Nowruzi, B., Wahlsten, M. and Jokela, J. 2019. A report on finding a new peptide aldehyde from cyanobacterium nostoc sp. Bahar m by lc-ms and marfey’s analysis. Iranian journal of biotechnology, 17(2).
Rajabpour, N., Nowruzi, B. and Ghobeh, M. 2019. Investigation of the toxicity, antioxidant and antimicrobial activities of some cyanobacterial strains isolated from different habitats. Acta Biologica Slovenica, 62: 2.
Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M. and Stanier, R.Y. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology, 111(1): 1-61.
Safavi, M., Nowruzi, B., Estalaki, S. and Shokri, M. 2019. Biological activity of methanol extract from nostoc sp. N42 and fischerella sp. S29 isolated from aquatic and terrestrial ecosystems. International Journal on Algae, 21(4).
Shokraei, R., Fahimi, H., Blanco, S. and Nowruzi, B. 2019. Genomic fingerprinting using highly repetitive sequences to differentiate close cyanobacterial strains. Microbial. Bioactiv, 2(1): 068-075.
Stanier, R., Kunisawa, R., Mandel, M. and Cohen-Bazire, G. 1971. Purification and properties of unicellular blue-green algae (order chroococcales). Bacteriological reviews, 35(2): 171.
Temraleeva, A., Dronova, S., Moskalenko, S. and Didovich, S. 2016. Modern methods for isolation, purification, and cultivation of soil cyanobacteria. Microbiology, 85(4): 389-399.
Uyeda, J.C., Harmon, L.J. and Blank, C.E. 2016. A comprehensive study of cyanobacterial morphological and ecological evolutionary dynamics through deep geologic time. PloS one, 11(9): e0162539.