Extraction of the breast cancer tissue under intraoperative radiotherapy and investigating their proteome by proteomics technique

Shahani, Minoo; Firouzi, Farideh

Document Type : Original Article

Authors

Cancer Research Center Shahid Beheshti Medical University

Abstract

Introduction and Aim: Breast cancer is the most common cancer among Iranian women and women in the world. The aim of radiotherapy (radiation therapy) is to eliminate the maximum of cancer cells with minimal damage to healthy tissue. Intraoperative radiation therapy (IORT) can be utilized to treat a small area of tissue without any damages. In this method, because of DNA damage and initiation of the apoptosis process, the tumor cells (target tissue) are destructed.
Methods: Four Breast Cancer (BC) patients as a pilot study were treated by 21 Gy (Radical dose). Two-dimensional electrophoresis (2D-PAGE) and Progenesis Same Spots v3.2 were performed to study the proteomic of the IORT-treated tumor bed.
Results: Approximately 523 protein spots were detected. A total of 343 protein spots showed different expression. among which, 141 spots decrease in-expression level, while, the remaining 191 spots were up-regulated.
Conclusion: Our findings indicate that Caspase3, P53, BID, BAX, and BCL2 may be key proteins in the IORT-treated tumor bed.

Keywords

20.1001.1.27832376.1399.1.1.4.8

Main Subjects

Cancer tissue and cell culture

References

Bahadur, Y.A. and Constantinescu, C.T. 2012. Tumor bed boost radiotherapy in breast cancer. Saudi Med J, 33(4): 353-366.

Chadha, M., Yoon, H., Feldman, S., Shah, N., Moore, E. and Harrison, L.B. 2009. Partial breast brachytherapy as the primary treatment for breast cancer diagnosed after mantle radiation therapy for hodgkin's disease. American journal of clinical oncology, 32(2): 132-136.

Deighton, R.F., McGregor, R., Kemp, J., McCulloch, J. and Whittle, I.R. 2010. Glioma pathophysiology: Insights emerging from proteomics. Brain Pathology, 20(4): 691-703.

Ginsburg, O., Dinh, N., To, T., Quang, L., Linh, N., Duong, B., Royer, R., Llacuachaqui, M., Tulman, A. and Vichodez, G. 2011. Family history, brca mutations and breast cancer in vietnamese women. Clinical genetics, 80(1): 89-92.

Kontoghiorghe, C.N., Andreou, N., Constantinou, K. and Kontoghiorghes, G.J. 2014. World health dilemmas: Orphan and rare diseases, orphan drugs and orphan patients. World journal of methodology, 4(3): 163.

Kraus-Tiefenbacher, U., Bauer, L., Scheda, A., Schoeber, C., Schaefer, J., Steil, V. and Wenz, F. 2007. Intraoperative radiotherapy (iort) is an option for patients with localized breast recurrences after previous external-beam radiotherapy. BMC cancer, 7(1): 178.

Lee, C.-L., Blum, J.M. and Kirsch, D.G. 2013. Role of p53 in regulating tissue response to radiation by mechanisms independent of apoptosis. Translational cancer research, 2(5): 412.

Matharoo‐Ball, B., Miles, A., Creaser, C., Ball, G. and Rees, R. 2008. Serum biomarker profiling in cancer studies: A question of standardisation? Veterinary and comparative oncology, 6(4): 224-247.

Olsson, H. 2000. Tumour biology of a breast cancer at least partly reflects the biology of the tissue/epithelial cell of origin at the time of initiation—a hypothesis. The Journal of Steroid Biochemistry and Molecular Biology, 74(5): 345-350.

Phillips, L.S. 2007. Breast ductal carcinoma in situ (dcis): Reproductive and hormonal risk factors and reliability of histologic diagnoses.

Ruck, I. and William, R. 1989. Intraoperative radiation therapy: Characterization and application. AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOOL OF ENGINEERING.

Tanca, A., Pagnozzi, D. and Addis, M.F. 2012. Setting proteins free: Progresses and achievements in proteomics of formalin‐fixed, paraffin‐embedded tissues. PROTEOMICS–Clinical Applications, 6(1‐2): 7-21.

Vitzthum, F., Behrens, F., Anderson, N.L. and Shaw, J.H. 2005. Proteomics: From basic research to diagnostic application. A review of requirements & needs. Journal of proteome research, 4(4): 1086-1097.

Yamauchi, H., Miyamura, K. and Abo, M. 2009. Proteomic assessment of important proteins for motor recovery in a rat model of photochemically-induced thrombosis. Journal of Applied Research, 9(4).

Zhang, L., Zhou, Z., Mei, X., Yang, Z., Ma, J., Chen, X., Wang, J., Liu, G., Yu, X. and Guo, X. 2015. Intraoperative radiotherapy versus whole-breast external beam radiotherapy in early-stage breast cancer: A systematic review and meta-analysis. Medicine, 94(27).

Zygogianni, G., Kyrgias, G., Kouvaris, J., Antypas, C., Skarlatos, J., Armpilia, C., Nikiteas, N. and Kouloulias, E. 2011. Intraoperative radiation therapy on pancreatic cancer patients: A review of the literature. Minerva chirurgica, 66(4): 361-369.

Research in Cell and Tissue

Extraction of the breast cancer tissue under intraoperative radiotherapy and investigating their proteome by proteomics technique

References

References

Volume 1, Issue 1
September 2020
Pages 26-33

Extraction of the breast cancer tissue under intraoperative radiotherapy and investigating their proteome by proteomics technique

References

References

Volume 1, Issue 1September 2020Pages 26-33

Volume 1, Issue 1
September 2020
Pages 26-33