Document Type : Original Article


1 Assistant professor, Agriculture Department, Production engineering and plant breeding Group, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran

2 Department of Biology, faculty of Sciences, University of Shiraz, Shiraz, Iran


Introduction and Aim: Low frequency of transformation and, more importantly, the occurrence of soma clonal variation at very high rates have considered disadvantages of Potato In vitro culture. Two Phytohormones, auxins (NAA, 2, 4-D), and cytokines (BAP, ZR) with concentrations were used to develop an efficient regeneration protocol for three genotypes of Potato.
Methods: The explants cultured on MS-medium supplemented with BAP 1 mg.l-1; NAA 0.1 mg.l-1; BAP 2 mg.l-1; NAA 0.2 mg.l-1; BAP 3 mg.l-1; NAA 0.3 mg.l-1; ZR 1 mg.l-1; 2.4-D 0.1 mg.l-1; ZR 2 mg.l-1; 2.4-D 0.2 mg.l-1; ZR 3 mg.l-1; 2.4-D 0.3 mg.l-1.
Results:The results showed that the highest percentage and number of root formation and shoot regeneration obtained in the medium included BAP 3 mg.l-1; NAA 0.3 mg.l-1 or ZR 3 mg.l-1; 2.4-D 0.3 mg.l-1 respectively. In this study, 0.3 mg.l-1 of both NAA and 2, 4-D were able to induce the most effective root regeneration.
Conclusion: The three potato genotypes were statistically different in their leaf regeneration efficiency. The Odyssey genotype showed a weak response to leaf regeneration.


Main Subjects

Asghari, F., Hossini, B., Hassani, A., Asghari, M. and Farokhi, J. 2013. Effect of different hormonal combination on in vitro direct shoot regeneration of basil (ocimum basilicum). Agricultural Biotechnology Journal, 4(2): 1-15.
Beaujean, A., Sangwan, R., Lecardonnel, A. and Sangwan-Norreel, B. 1998. Agrobacterium-mediated transformation of three economically important potato cultivars using sliced internodal explants: An efficient protocol of transformation. Journal of experimental Botany, 49(326): 1589-1595.
Begum, F., Amin, M. and Azad, M. 2002. In vitro rapid clonal propagation of ocimum basilicum l.
Bipasha, C. and Wang-Pruski, G. 2010. Rapid regeneration of stable transformants in cultures of potato by improving factors influencing agrobacterium-mediated transformation. Advances in Bioscience and Biotechnology, 1(5): 409-416.
Chen, S.-C., Liu, H.-W., Lee, K.-T. and Yamakawa, T. 2007. High-efficiency agrobacterium rhizogenes-mediated transformation of heat inducible shsp18. 2-gus in nicotiana tabacum. Plant cell reports, 26(1): 29-37.
De Block, M. 1988. Genotype-independent leaf disc transformation of potato (solanum tuberosum) using agrobacterium tumefaciens. Theoretical and applied genetics, 76(5): 767-774.
Esfahani, K., MOTALEBI, M., Zamani, M.R., HASHEMI, S.H. and Jourabchi, E. 2010. Transformation of potato (solanum tuberosum cv. Savalan) by chitinase and β-1, 3-glucanase genes of mycoparasitic fungi towards improving resistance to rhizoctonia solani ag-3.
Fatima, N., Ahmad, N., Ahmad, I. and Anis, M. 2015. Interactive effects of growth regulators, carbon sources, ph on plant regeneration and assessment of genetic fidelity using single primer amplification reaction (spars) techniques in withania somnifera l. Applied biochemistry and biotechnology, 177(1): 118-136.
Fatima, N. and Anis, M. 2012. Role of growth regulators on in vitro regeneration and histological analysis in indian ginseng (withania somnifera l.) dunal. Physiology and molecular biology of plants, 18(1): 59-67.
Gerdakaneh, M., Badakhshan, H., Mohamadi, M. and Arji, I. 2020. Effect of different media and growth regulators on micropropagation of gf677. Journal of Plant Productions (Agronomy, Breeding and Horticulture), 43(2): 241-254.
Hijmans, R.J. and Spooner, D.M. 2001. Geographic distribution of wild potato species. American journal of Botany, 88(11): 2101-2112.
Hossein, P. and Nikolaevich, E.A. 2015. Statistical analysis of growth factors in potato during regeneration with different hormonal treatments. Austrian Journal of Technical and Natural Sciences(11-12).
Imai, T., Aida, R. and Ishige, T. 1993. High frequency of tetraploidy in agrobacterium-mediated transformants regenerated from tuber discs of diploid potato lines. Plant cell reports, 12(6): 299-302.
Ishida, B.K., Snyder, G.W. and Belknap, W.R. 1989. The use of in vitro-grown microtuber discs in agrobacterium-mediated transformation of russet burbank and lemhi russet potatoes. Plant cell reports, 8(6): 325-328.
Kharrazi, M., Sharifi, A., Keykha Akhar, F., Bagheri, A. and Moradian, Y. 2018. Effect of hormonal compositions on micropropagation of fifteen cultivars of gerbera (gerbera jamesonii bolus ex hooker f.). Journal of Plant Productions (Agronomy, Breeding and Horticulture), 40(4): 91-102.
Mohamadzadeh, M.N., Safipour, A.A. and Saeid, N.F. 2019. The effects of medium, sterilization and hormonal treatment on micropropagation of some apple (mallus domestica borkh.) rootstocks.
Muktadir, M.A., Habib, M.A., Mian, M.A.K. and Akhond, M.A.Y. 2016. Regeneration efficiency based on genotype, culture condition and growth regulators of eggplant (solanum melongena l.). Agriculture and Natural Resources, 50(1): 38-42.
Olivera-Ortega, V., Gutiérrez-Espinosa, M. and Andrade-Rodríguez, M. 2000. In vitro culture of gerbera (gerbera jamesonii h. Bolus) and its acclimation in greenhouse. Bioagro, 12(3): 75-80.
Ooms, G., Burrell, M., Karp, A., Bevan, M. and Hille, J. 1987. Genetic transformation in two potato cultivars with t-DNA from disarmed agrobacterium. Theoretical and Applied Genetics, 73(5): 744-750.
Pasalari, H. 2020. Evaluating the resistance of potato tubers under different temperatures related to potato bacterial soft rot disease control. Plant Protection, 43(3): 35-42.
Sahoo, Y., Pattnaik, S. and Chand, P. 1997. In vitro clonal propagation of an aromatic medicinal herb ocimum basilicum l.(sweet basil) by axillary shoot proliferation. In Vitro Cellular & Developmental Biology-Plant, 33(4): 293-296.
Van Eck, J. and Kitto, S. 1992. Regeneration of peppermint and orange mint from leaf disks. Plant Cell, Tissue and Organ Culture, 30(1): 41-49.