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Evaluation effects of silymarin on cytotoxicity and apoptosis on SW480 colon cancer cell line

Document Type : Original Article

Authors

1 Pharmaceutical sciences center.Tehran medical sciences, Islamic Azad University, Tehran,Iran

2 Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

3 Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Abstract

Introduction and Aim: Silymarin, extracted from the milk thistle, is rich in flavolignans such as silybinin, silidianin, and silycristine. Several studies have been done on its anticancer effect. This study aimed to evaluate the effects of cytotoxicity and induction of silymarin-induced apoptosis on colon cancer SW480 and normal HEK-293 cell lines.
Methods: In the present study, the effect of cytotoxicity and apoptosis induction by silymarin on colon cancer SW480 and Normal HEK-293 cell lines were investigated by different methods such as trypan blue staining, MTT assay, Annexin V/PI staining, and evaluating the expression level of BAX and BCL2 Genes by q-PCR technique.
Results: The results of the trypan blue staining and MTT assay indicated the cytotoxicity effects of silymarin in SW480 cells in a time-dependent manner. The IC50 was also evaluated. However, no cytotoxic effects were observed in HEK-293 cells after treatment with silymarin. Also, an increase of apoptosis in SW480 cells that were treated with 50 and 100 µg/mL silymarin was observed. An increase in BAX/BCL2 gene expression level was also observed in SW480 cells compared to HEK-293 cells.
Conclusion: According to the results of the present study, silymarin can induce toxicity and cell apoptosis in the colon cancer cell line without causing cytotoxicity in the normal cell line. So silymarin can be suggested for effective treatment of colon cancer.

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References
Bayram, D., Çetin, E.S., Kara, M., Özgöçmen, M. and Candan, I. 2017. The apoptotic effects of silibinin on mda-mb-231 and mcf-7 human breast carcinoma cells. Human & experimental toxicology, 36(6): 573-586.
Chirumbolo, S., Bjørklund, G., Lysiuk, R., Vella, A., Lenchyk, L. and Upyr, T. 2018. Targeting cancer with phytochemicals via their fine tuning of the cell survival signaling pathways. International journal of molecular sciences, 19(11): 3568.
Choi, E.-S., Oh, S., Jang, B., Yu, H.-J., Shin, J.-A., Cho, N.-P., Yang, I.-H., Won, D.-H., Kwon, H.-J. and Hong, S.D. 2017. Silymarin and its active component silibinin act as novel therapeutic alternatives for salivary gland cancer by targeting the erk1/2-bim signaling cascade. Cellular Oncology, 40(3): 235-246.
de Avelar, C.R., Pereira, E.M., de Farias Costa, P.R., de Jesus, R.P. and de Oliveira, L.P.M. 2017. Effect of silymarin on biochemical indicators in patients with liver disease: Systematic review with meta-analysis. World journal of gastroenterology, 23(27): 5004.
Deep, G., Singh, R., Agarwal, C., Kroll, D. and Agarwal, R. 2006. Silymarin and silibinin cause g1 and g2–m cell cycle arrest via distinct circuitries in human prostate cancer pc3 cells: A comparison of flavanone silibinin with flavanolignan mixture silymarin. Oncogene, 25(7): 1053-1069.
Delmas, D., Xiao, J., Vejux, A. and Aires, V. 2020. Silymarin and cancer: A dual strategy in both in chemoprevention and chemosensitivity. Molecules, 25(9): 2009.
Farag, R.S., Abdel-Latif, M.S., Abd El Baky, H.H. and Tawfeek, L.S. 2020. Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices. Biotechnology Reports, 28: e00536.
Federico, A., Dallio, M. and Loguercio, C. 2017. Silymarin/silybin and chronic liver disease: A marriage of many years. Molecules, 22(2): 191.
Gheybi, F., Alavizadeh, S.H., Rezayat, S.M., Zendedel, E. and Jaafari, M. 2019. Chemotherapeutic activity of silymarin combined with doxorubicin liposomes in 4t1 breast cancer cells. Nanomedicine Research Journal, 4(1): 29-34.
Girardi, B., Principi, M., Pricci, M., Giorgio, F., Iannone, A., Losurdo, G., Ierardi, E., Di Leo, A. and Barone, M. 2018. Chemoprevention of inflammation-related colorectal cancer by silymarin-, acetyl-11-keto-beta-boswellic acid-, curcumin-and maltodextrin-enriched dietetic formulation in animal model. Carcinogenesis, 39(10): 1274-1282.
Hagag, A.A., Elgamsy, M.A., El-Asy, H.M. and Mabrouk, M.M. 2016. Protective role of silymarin on hepatic and renal toxicity induced by mtx based chemotherapy in children with acute lymphoblastic leukemia. Mediterranean journal of hematology and infectious diseases, 8(1).
Hekmat, A., Salavati, F. and Tackallou, S.H. 2020. The effects of paclitaxel in the combination of diamond nanoparticles on the structure of human serum albumin (hsa) and their antiproliferative role on mda-mb-231cells. The Protein Journal: 1-16.
Iqbal, B., Ali, J., Ganguli, M., Mishra, S. and Baboota, S. 2019. Silymarin-loaded nanostructured lipid carrier gel for the treatment of skin cancer. Nanomedicine, 14(9): 1077-1093.
Kim, S.H., Choo, G.S., Yoo, E.S., Woo, J.S., Han, S.H., Lee, J.H. and Jung, J.Y. 2019. Silymarin induces inhibition of growth and apoptosis through modulation of the mapk signaling pathway in ags human gastric cancer cells. Oncology reports, 42(5): 1904-1914.
Mehta, A. and Patel, B.M. 2019. Therapeutic opportunities in colon cancer: Focus on phosphodiesterase inhibitors. Life sciences, 230: 150-161.
Pashah, Z., Hekmat, A. and Hesami Tackallou, S. 2019. Structural effects of diamond nanoparticles and paclitaxel combination on calf thymus DNA. Nucleosides, Nucleotides and Nucleic Acids, 38(4): 249-278.
Shi, Z., Zhou, Q., Gao, S., Li, W., Li, X., Liu, Z., Jin, P. and Jiang, J. 2019. Silibinin inhibits endometrial carcinoma via blocking pathways of stat3 activation and srebp1-mediated lipid accumulation. Life sciences, 217: 70-80.
Siegel, R.L., Miller, K.D., Goding Sauer, A., Fedewa, S.A., Butterly, L.F., Anderson, J.C., Cercek, A., Smith, R.A. and Jemal, A. 2020. Colorectal cancer statistics, 2020. CA: a cancer journal for clinicians, 70(3): 145-164.
Soodvilai, S., Tipparos, W., Rangsimawong, W., Patrojanasophon, P., Soodvilai, S., Sajomsang, W. and Opanasopit, P. 2019. Effects of silymarin-loaded amphiphilic chitosan polymeric micelles on the renal toxicity and anticancer activity of cisplatin. Pharmaceutical development and technology, 24(8): 927-934.
Tuli, H.S., Mittal, S., Aggarwal, D., Parashar, G., Parashar, N.C., Upadhyay, S.K., Barwal, T.S., Jain, A., Kaur, G. and Savla, R. 2020. Path of silibinin from diet to medicine: A dietary polyphenolic flavonoid having potential anti-cancer therapeutic significance. In: Seminars in Cancer Biology. Elsevier.
Won, D.-H., Kim, L.-H., Jang, B., Yang, I.-H., Kwon, H.-J., Jin, B., Oh, S.H., Kang, J.-H., Hong, S.-D. and Shin, J.-A. 2018. In vitro and in vivo anti-cancer activity of silymarin on oral cancer. Tumor Biology, 40(5): 1010428318776170.
Research in Cell and Tissue
Volume 1, Issue 3
March 2021
Pages 8-15
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History
  • Receive Date: 12 September 2020
  • Revise Date: 10 December 2020
  • Accept Date: 21 February 2021
  • First Publish Date: 21 February 2021
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