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اثر ضد تکثیری سدیم بوتیرات بر رده سلولی Caco-2 سرطان کولورکتال انسانی با کاهش نسبت بیان رونوشت ژن BcL-2 به Bax

نوع مقاله : مقاله پژوهشی

نویسندگان

1 هیات علمی، دانشگاه علوم پزشکی آزاد اسلامی تهران، دانشکده علوم و فناوری های نوین

2 گروه ژنتیک، دانشکده علوم و فناوری های نوین، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران

3 گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

چکیده

پیشینه مطالعه و هدف: سرطان کولورکتال یکی از شایع‌ترین سرطان‌ها است و تغییرات اپی‌ژنتیکی به عنوان هدف درمانی مورد توجه قرار گرفته است. سدیم بوتیرات با هایپر-استیلاسیون اجزا کروماتین قادر به تغییر در بیان ژن‌ها است. هدف از این مطالعه بررسی تاثیر سدیم بوتیرات بر روی بیان ژن‌های Bax و Bcl-2 می‌باشد.
روش مطالعه: رده سلولی Caco-2 با غلظت‌های مختلف سدیم بوتیرات (25 الی 150 میلی‌مولار) براساس غلظت IC50 در دو بازه زمانی 24 ساعت و 48 ساعت تیمار شد. بیان ژن‌های Bax و Bcl-2 توسط تکنیک qReal-Time PCR به روش ΔΔCT -2 اندازه‌گیری و نسبت Bcl-2/Bax مورد ارزیابی قرار گرفت.
نتایج: نتایج نشان داد سدیم بوتیرات باعث افزایش بیان ژن Bax و کاهش بیان ژن Bcl2 در سلول‌های تحت تیمار در مقایسه با گروه کنترل شد که از نظر آماری معنی‌دار می‌باشد (p <0.05). غلظت 25 میلی‌مولار در بازه زمانی 48 ساعت تیمار موثرترین دوز انتخاب گردید. همچنین نسبت Bcl-2/Bax در همین غلظت کاهش معنی‌داری را نشان داد.
نتیجه‌گیری: سدیم بوتیرات با کاهش نسبت بیان Bcl-2/Bax باعث القا آپوپتوز در سلول‌های سرطانی می‌شود. این ماده می‌تواند به عنوان هدف درمانی استفاده شود ولی نیاز به بررسی بیشتر می‌باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Anti-proliferative effect of sodium butyrate against Caco-2 cell line of human colorectal cancer by reducing the expression ratio of BcL-2 to Bax mRNA

نویسندگان [English]

  • Flora Forouzesh 1
  • Pantea Hajimirza Shafiesoltani 2
  • Mahsa Ghiaghi 2
  • Mahdi Shabani 3

1 Assistant Professor Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

2 Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

3 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

چکیده [English]

Introduction and Aim: Colorectal cancer is one of the most common cancers. Epigenetic change has been considered by many scientists as a therapeutic target. Hyper acetylation of chromatin components by sodium butyrate can alter gene regulation. This study aims to investigate the effects of sodium butyrate on Bax and Bcl-2 gene expression.
Methods: Caco-2 cell line was treated with different concentrations of sodium butyrate (25 mM to 150 mM) based on IC50 concentration in two time periods of 24 hours and 48 hours. Bax and Bcl-2 gene expression were measured by qReal-Time PCR technique and Bcl2/Bax ratio was evaluated.
Results: The results showed that sodium butyrate increased the expression of Bax gene and decreased the expression of Bcl-2 gene in treated cells compared to the control group, which was statistically significant (p < /em> <0.05), and 25 mM was selected as the most effective dose after 48 hours of treatment. Also, the Bcl-2/Bax ratio at the same concentration showed a significant decrease
Conclusion: Sodium butyrate induces apoptosis in cancer cells by reducing the expression ratio of Bcl-2/Bax. It can be used as a therapeutic target but needs further investigation.

کلیدواژه‌ها [English]

  • Colorectal cancer
  • Sodium butyrate
  • Caco-2 cell line
  • Bax
  • Bcl2
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پژوهش‌هایی در یاخته و بافت
دوره 1، شماره 3
اسفند 1399
صفحه 16-24
فایل ها
سابقه مقاله
  • تاریخ دریافت: 12 مرداد 1399
  • تاریخ بازنگری: 20 آبان 1399
  • تاریخ پذیرش: 30 بهمن 1399
  • تاریخ اولین انتشار: 01 اسفند 1399
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