نوع مقاله : مقاله پژوهشی

نویسندگان

1 Pharmaceutical sciences center,Tehran medical sciences, Islamic Azad University, Tehran, Iran

2 Faculty of Biological Sciences and Technology, Shahid Beheshti University of Tehran, Iran

3 Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

چکیده

Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis induction of 7-hydroxycycoumarin were investigated on the SW480 colon cancer cell line.
Methods: anti-proliferative effect of 7-hydroxycoumarin was investigated using Trypan blue and MTT assay at different concentrations on SW480 cell line. Additionally, apoptosis induction was examined by flow cytometry and real-time PCR.
Results: The results show a decrease in viability. IC50 concentrations were observed at the concentrations of 164.2, 80.48, and 41.97 μg/ml for 24, 48, and 72 h, respectively. Of note, IC50 concentrations were not observed in the trypan blue assay. As well, there was a 2.4-fold increase in apoptosis (P<0.001) using Annexin / PI kit by flow cytometry. as well as the increased expression of BAX/BCL2 ratio of about 13.69, which was observed at 100 μg/ml compared to the control.
Discussion: The results indicate that 7-hydroxycoumarin could inhibit cell proliferation and induce apoptosis in the SW480 cell line. Therefore, 7-hydroxycycoumarin can be considered as an anti-cancer agent in the complementary treatment of colon cancer.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Cytotoxicity and Apoptosis Induction of 7- Hydroxycoumarin on SW480 Colon Cancer Cell Line

نویسندگان [English]

  • Hossein Gravand 1
  • Jafar Kazemian 2
  • Masoumeh Heshmati 3

1 Pharmaceutical sciences center, Tehran medical sciences, Islamic Azad University, Tehran, Iran

2 Faculty of Biological Sciences and Technology, Shahid Beheshti University of Tehran, Iran

3 Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

چکیده [English]

Introduction: Colorectal cancer is a common disease ranked as the third-highest deathly cancer worldwide. In recent years, for the treatment of cancers scientists have preferred herbal remedies because of their better effects and fewer side effects. So, in this study, the cytotoxicity effect and apoptosis induction of 7-hydroxycycoumarin were investigated on the SW480 colon cancer cell line.
Methods: anti-proliferative effect of 7-hydroxycoumarin was investigated using Trypan blue and MTT assay at different concentrations on SW480 cell line. Additionally, apoptosis induction was examined by flow cytometry and real-time PCR.
Results: The results show a decrease in viability. IC50 concentrations were observed at the concentrations of 164.2, 80.48, and 41.97 μg/ml for 24, 48, and 72 h, respectively. Of note, IC50 concentrations were not observed in the trypan blue assay. As well, there was a 2.4-fold increase in apoptosis (P<0.001) using Annexin / PI kit by flow cytometry. as well as the increased expression of BAX/BCL2 ratio of about 13.69, which was observed at 100 μg/ml compared to the control.
Discussion: The results indicate that 7-hydroxycoumarin could inhibit cell proliferation and induce apoptosis in the SW480 cell line. Therefore, 7-hydroxycycoumarin can be considered as an anti-cancer agent in the complementary treatment of colon cancer.

کلیدواژه‌ها [English]

  • Keywords: Apoptsis
  • Colon cancer
  • Cytotoxicity
  • 7- hydroxycoumarin
Beillerot, A., Domínguez, J.-C.R., Kirsch, G. and Bagrel, D. 2008. Synthesis and protective effects of coumarin derivatives against oxidative stress induced by doxorubicin. Bioorganic & medicinal chemistry letters, 18(3): 1102-1105.
Bisi, A., Cappadone, C., Rampa, A., Farruggia, G., Sargenti, A., Belluti, F., Di Martino, R.M., Malucelli, E., Meluzzi, A. and Iotti, S. 2017. Coumarin derivatives as potential antitumor agents: Growth inhibition, apoptosis induction and multidrug resistance reverting activity. European journal of medicinal chemistry, 127: 577-585.
Devji, T., Reddy, C., Woo, C., Awale, S., Kadota, S. and Carrico-Moniz, D. 2011. Pancreatic anticancer activity of a novel geranylgeranylated coumarin derivative. Bioorganic & medicinal chemistry letters, 21(19): 5770-5773.
Dillard, C.J. and German, J.B. 2000. Phytochemicals: Nutraceuticals and human health. Journal of the Science of Food and Agriculture, 80(12): 1744-1756.
Giovannucci, E. 2002. Modifiable risk factors for colon cancer. Gastroenterology Clinics, 31(4): 925-943.
Issa, I.A. and Noureddine, M. 2017. Colorectal cancer screening: An updated review of the available options. World journal of gastroenterology, 23(28): 5086.
Küpeli Akkol, E., Genç, Y., Karpuz, B., Sobarzo-Sánchez, E. and Capasso, R. 2020. Coumarins and coumarin-related compounds in pharmacotherapy of cancer. Cancers, 12(7): 1959.
Lagoa, R., Silva, J., Rodrigues, J.R. and Bishayee, A. 2020. Advances in phytochemical delivery systems for improved anticancer activity. Biotechnology advances, 38: 107382.
Lopez-Gonzalez, J.S., Prado-Garcia, H., Aguilar-Cazares, D., Molina-Guarneros, J.A., Morales-Fuentes, J. and Mandoki, J.J. 2004. Apoptosis and cell cycle disturbances induced by coumarin and 7-hydroxycoumarin on human lung carcinoma cell lines. Lung cancer, 43(3): 275-283.
Marshall, M., Kervin, K., Benefield, C., Umerani, A., Albainy-Jenei, S., Zhao, Q. and Khazaeli, M. 1994. Growth-inhibitory effects of coumarin (1, 2-benzopyrone) and 7-hydroxycoumarin on human malignant cell lines in vitro. Journal of cancer research and clinical oncology, 120(1): S3-S10.
Mirunalini, S., Deepalakshmi, K. and Manimozhi, J. 2014. Antiproliferative effect of coumarin by modulating oxidant/antioxidant status and inducing apoptosis in hep2 cells. Biomedicine & Aging Pathology, 4(2): 131-135.
Musa, M.A., Badisa, V.L., Latinwo, L.M., Cooperwood, J., Sinclair, A. and Abdullah, A. 2011. Cytotoxic activity of new acetoxycoumarin derivatives in cancer cell lines. Anticancer research, 31(6): 2017-2022.
Önder, A. 2020. Anticancer activity of natural coumarins for biological targets. Studies in Natural Products Chemistry, 64: 85-109.
Rafiemanesh, H., Pakzad, R., Abedi, M., Kor, Y., Moludi, J., Towhidi, F., Makhsosi, B.R. and Salehiniya, H. 2016. Colorectal cancer in iran: Epidemiology and morphology trends. EXCLI journal, 15: 738.
Saidu, N.E.B., Valente, S., Bana, E., Kirsch, G., Bagrel, D. and Montenarh, M. 2012. Coumarin polysulfides inhibit cell growth and induce apoptosis in hct116 colon cancer cells. Bioorganic & medicinal chemistry, 20(4): 1584-1593.
Sung, H., Ferlay, J., Siegel, R.L., Laversanne, M., Soerjomataram, I., Jemal, A. and Bray, F. 2021. Global cancer statistics 2020: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians, 71(3): 209-249.
Vardanjani, H.M., Haghdoost, A., Bagheri-Lankarani, K. and Hadipour, M. 2018. Estimation and projection of prevalence of colorectal cancer in iran, 2015–2020. Advanced biomedical research, 7.
Weston, C.R., Balmanno, K., Chalmers, C., Hadfield, K., Molton, S.A., Ley, R., Wagner, E.F. and Cook, S.J. 2003. Activation of erk1/2 by δraf-1: Er* represses bim expression independently of the jnk or pi3k pathways. Oncogene, 22(9): 1281-1293.