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Isolation and evaluation of human adipose tissue characterization for preparation of vascular stem cells

Document Type : Original Article

Authors

1 Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran

3 Department of Hematology, Faculty of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Introduction and Aim: Several studies have been carried out on the biological characteristics of a human adipose tissue-derived-stromal vascular fraction. However, investigating this fraction concerning its clinical application is still of significant importance. The aim of this study was to isolate and evaluate the cell population of human adipose stromal vascular fraction with the main aim of investigating the presence of stem cells in human adipose stromal vascular fraction.
Methods: In this laboratory-experimental study adipose tissues were obtained from 10 healthy individuals (30 to 58 years) and maintained in standard condition. After enzymatic isolation, the viability of stromal vascular fraction cells and surface antigens was evaluated by flow cytometry.
Results: The results of this study showed that adipose stromal vascular fraction cells had high viability (> 98%) and were positive for CD16, CD34, CD73, CD29, CD105, CD31, and CD45 markers and negative for CD3, CD19, and CD38 markers.
Conclusion: The findings of this study indicate the presence of adipose-derived stem cells, endothelial cells, peri-adipocytes, and macrophages. According to this, this fraction has potential in the clinical field of cell therapy.

Keywords

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References
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Research in Cell and Tissue
Volume 2, Issue 1
August 2021
Pages 8-14
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History
  • Receive Date: 26 January 2021
  • Revise Date: 03 April 2021
  • Accept Date: 09 June 2021
  • First Publish Date: 09 June 2021
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