Document Type : Original Article
1 Department of Pilot Biotechnology, Pasteur Institute, Tehran, Iran
2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Introduction and Aim: Human chorionic gonadotropin (hCG) is a glycoprotein produced during pregnancy by trophoblast and placental syncytiotrophoblast cells. The produced hCG hormone affects the corpus luteum and prevents it from being destroyed. Studies show that diamond nanoparticles (NDs) interact with biological systems such as proteins, enzymes, and antibodies. In other words, NDs are capable of being absorbed by biomolecules and can also be used as a carrier for the transport of biologically active substances. This study aimed to investigate the effect of NDs on hCG structure and on hCG level in Wistar rats.
Methods: The effect of 10 nm NDs on the structure of hCG was investigated using spectroscopic techniques. Rats were randomly divided into five groups: Control, sham, and experimental groups (received NDs solution at doses of 25, 75, and 100 mg/ml). Injections were carried out twice a week. Then, serum biochemical parameters were calculated. Data were analyzed utilizing one-way ANOVA followed by Tukey's multiple comparisons test.
Results: The results showed that NDs could form a complex with hCG. The zeta potential and DLS results showed that NDs cause a decrease in the surface charge and size of hCG. Administration of NDs in concentrations of 100 mg/ml significantly decreased hCG levels compared with that of control samples (P<0.05),
Conclusion: Based on the investigation NDs could change the tertiary structure of hCG. NDs had toxic effects on hCG function. More studies to explore the toxic effects of NDs in various doses and times of exposure seem to be necessary.