نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه، واحد همدان، دانشگاه آزاد اسلامی، همدان، ایران

2 گروه بیولوژی، کالج بین المللی اویسینا، بوداپست، مجارستان

3 گروه روانشناسی بالینی، دانشکده علوم پزشکی، واحد تنکابن، دانشگاه آزاد تنکابن، ایران

چکیده

پیشینه مطالعه و هدف: مطالعات بسیاری نشان دهنده ارتباط امواج فراصوت با عملکرد سیستم تولید مثلی می‌باشند. در عین حال مکانیسم اثر امواج فراصوت بر هورمون‌های جنسی و بافت بیضه کاملا روشن نیست. بر این اساس مطالعه حاضر بررسی اثرات امواج فراصوت بر سطح سرمی هورمون‌های DHEA-SO4، تستوسترون و بافت سلولی بیضه در موش صحرایی نر پرداخته است.
روش مطالعه: موش‌های نر نژاد ویستار در سه گروه تقسیم شدند: گروه کنترل که در معرض امواج فراصوتی نبودند، موش‌هایی که یک ساعت در روز در معرض امواج فراصوت بودند، و موش‌هایی که شش ساعت در روز در معرض امواج فراصوت بودند. سطح سرمی تستوسترون و DHEA-SO4 به روش الایزا اندازه گیری شد. اسلایدهای بافت بیضه با استفاده از میکروسکوپ مورد تجزیه و تحلیل قرار گرفت. داده‌ها با استفاده از آزمون آنالیز واریانس یک‌طرفه مقایسه شدند.
نتایج: سطح سرمی هورمون‌های تستوسترون و DHEA-SO4 ، تعداد سلول‌های اسپرماتوگونی، سلول‌های اسپرماتوسیت و سلول‌های سرتولی و مورفولوژی لوله‌های اسپرم‌ساز بافت بیضه در گروه‌های در معرض امواج فراصوت در مقایسه با گروه کنترل تفاوت معناداری نداشت.
نتیجه‌گیری: یافته‌های مطالعه نشان داد که امواج فراصوت با فرکانس پایین بر عملکرد و بافت‌شناسی سیستم تناسلی نر تاثیر معناداری ندارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigated the effects of ultrasound waves on serum levels of DHEA-SO4, testosterone, and testicular tissue in male rats

نویسندگان [English]

  • Mohammad Karamali Gilaneh 1
  • Rahim Ahmadi 1 2
  • Fatemeh Amini Khodashahri 3

1 Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran

2 Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran

3 Department of Clinical Psychology, Faculty of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

چکیده [English]

Introduction: Many studies have shown the relationship between ultrasound and the function of the reproductive system. However, the mechanism of ultrasound waves action on sex hormones and testicular tissue is not perfectly clear. Accordingly, the present study investigated the effects of ultrasound waves on serum levels of DHEA-SO4, testosterone and testicular tissue in male rats.
Materials and Method: Male wistar rats were divided into three groups: control group which was not exposed to ultrasound waves, rats that were exposed to ultrasound waves for 1h/day, and rats that were exposed to ultrasound waves for 6h/day. The serum levels of testosterone and DHEA-SO4 were measured using ELISA. The testicular tissue slides were prepared and analyzed microscopically.Data were analyzed using one-way ANOVA.
Results: Serum levels of testosterone and DHEA-SO4 and spermatogonia, spermatocytes, sertoli cell count and seminiferous tubules morphology did not significantly changed in groups exposed to ultrasound waves compared to control animals.
Conclusion: Our findings show that ultrasound waves do not significantly influence male reproductive system function and histology.

کلیدواژه‌ها [English]

  • Ultrasound waves
  • Testosterone
  • DHEA-SO4
  • Testis tissue
  • Rat
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