Document Type : Original Article


1 Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran

2 Department of Biology, Avicenna International College, Budapest, Hungary



Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in human red blood cells.
Methods: In this experimental laboratory study, blood samples were obtained from healthy individuals, and blood samples were divided into the control group (treated with normal saline) and 2, 4, and 6 mg/kg of sulfasalazine receiving groups. Samples treated with 2 mg/kg of sulfasalazine were treated with 2, 4, and 6 mg/kg of Pissum sativum L and Aloe vera extract, and the erythrocyte membrane stability was calculated by standard methods. Data were analyzed using a one-way analysis of variance.
Results: Sulfasalazine significantly reduced the stability of the erythrocyte membrane compared to the control group. Treatment of samples with concentrations of 4 and 6 mg/kg Pissum sativum L extract significantly increased membrane stability compared to the groups treated with sulfasalazine. Treatment with 2 and 4 mg/kg of Aloe vera extract did not increase membrane stability and a 6 mg/kg of Aloe vera extract non-significantly increased the membrane stability.
Conclusion: Unlike Aloe vera, Pissum sativum L extract can increase the membrane stability of red blood cells in healthy people.


Main Subjects

Langmead, L., Makins, R.J. and Rampton, D.S. 2004. Anti‐inflammatory effects of aloe vera gel in human colorectal mucosa in vitro. Alimentary pharmacology & therapeutics, 19(5): 521-527.
Martínez, J.E.B., Concha, D.d.R.M., Velázquez, T.G.G., Martínez, C.J. and Ruiz, J.C.R. 2021. Anti-inflammatory properties of phenolic extracts from phaseolus vulgaris and pisum sativum during germination. Food Bioscience, 42: 101067.
Michel, F., Navellou, J.-C., Ferraud, D., Toussirot, E. and Wendling, D. 2005. Dress syndrome in a patient on sulfasalazine for rheumatoid arthritis. Joint Bone Spine, 72(1): 82-85.
Paul, S., Modak, D., Chattaraj, S., Nandi, D., Sarkar, A., Roy, J., Chaudhuri, T.K. and Bhattacharjee, S. 2021. Aloe vera gel homogenate shows anti-inflammatory activity through lysosomal membrane stabilization and downregulation of tnf-α and cox-2 gene expressions in inflammatory arthritic animals. Future Journal of Pharmaceutical Sciences, 7(1): 1-8.
Pirmohamed, M., Coleman, M., Hussain, F., Breckenridge, A. and Park, B. 1991. Direct and metabolism‐dependent toxicity of sulphasalazine and its principal metabolites towards human erythrocytes and leucocytes. British journal of clinical pharmacology, 32(3): 303-310.
Rajasekaran, S., Sivagnanam, K. and Subramanian, S. 2005. Antioxidant effect of aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacol Rep, 57(1): 90-96.
Ratnayake, W., Hoover, R., Shahidi, F., Perera, C. and Jane, J. 2001. Composition, molecular structure, and physicochemical properties of starches from four field pea (pisum sativum l.) cultivars. Food chemistry, 74(2): 189-202.
Sese-Owei, E., Enagbonma, B.J. and Ekiyor, C. 2020. Haematological parameters of albino rats exposed to lead metal: Alleviating effect of cocos nucifera l. Water and pisum sativum extract. NISEB Journal, 19(2).
Surjushe, A., Vasani, R. and Saple, D. 2008. Aloe vera: A short review. Indian journal of dermatology, 53(4): 163.
Svetina, S., Kuzman, D., Waugh, R.E., Ziherl, P. and Žekš, B. 2004. The cooperative role of membrane skeleton and bilayer in the mechanical behaviour of red blood cells. Bioelectrochemistry, 62(2): 107-113.
Teplitsky, V., Virag, I. and Halabe, A. 2000. Immune complex haemolytic anaemia associated with sulfasalazine. BMJ, 320(7242): 1113.