Document Type : Original Article


1 Department of Basic Sciences School of Pharmacy and Pharmaceutical Sciences, Tehran University of Medical Sciences, Islamic Azad University, Tehran, Iran

2 Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran University of Medical Sciences, Islamic Azad University, Tehran, Iran

3 Chronic Respiratory Diseases Research Center, Tuberculosis and Pulmonary Diseases Research Institute, Tuberculosis and Pulmonary Diseases Research Center, Masih Daneshvari Hospital Shahid Beheshti University of Medical Sciences, Tehran, Iran.



Introduction and aim: Genes play a very important role in the development of cancer. This study aimed to evaluate three very important genes (C-fos, C-jun, and P53) in Wistar mice's stomach tissue cancer cells designed by dimethylhydrazine.
Methods: In this study, forty male Wistar mice, 57 weeks old, were evaluated and divided into four groups of Ten. In the control group, the second group received Cinnamaldehyde-free carcinogens, the third group was healthy with the Cinnamaldehyde, and the fourth group received carcinogens with the Cinnamaldehyde at the same time. Then, the gastric tissue of mice was isolated and evaluated for the expression of C-fos, C-Jun, and P53 genes. The data were analyzed with One-way ANOVA using SPSS.
Results: Statistical analysis of the results showed that the expression of C-fos, C-Jun, and P53 genes in the carcinogen receptor with the Cinnamaldehyde was increased compared to the control group. This increase was statistically significant for two genes C-fos (P = 0.0146) and P53 (P = 0.0212) and for the C-Jun gene (P = 0.01604) this increase was not significant.
Conclusion: The results of this study showed that Cinnamaldehyde can alter the expression of important genes C-fos, C-Jun, and P53 that are effective in causing gastric cancer.


Main Subjects

Baranovsky, A. and Myers, M.H. 1986. Cancer incidence and survival in patients 65 years of age and older. CA: a cancer journal for clinicians, 36(1): 26-41.
Cabello, C.M., Bair III, W.B., Lamore, S.D., Ley, S., Bause, A.S., Azimian, S. and Wondrak, G.T. 2009. The cinnamon-derived michael acceptor cinnamic aldehyde impairs melanoma cell proliferation, invasiveness, and tumor growth. Free Radical Biology and Medicine, 46(2): 220-231.
Dejakam, A., Dejakam, A. and Hekmat, A. 2020. Evaluating of bdnf expression in blood cells of opium recovering patients with a new treatment method: A molecular marker. Research in Karyotic Cell & Tissue, 1(1): 16-25.
Edwards, J., Krishna, N.S., Mukherjee, R. and Bartlett, J.M. 2004. The role of c‐jun and c‐fos expression in androgen‐independent prostate cancer. The Journal of Pathology: A Journal of the Pathological Society of Great Britain and Ireland, 204(2): 153-158.
Eferl, R., Ricci, R., Kenner, L., Zenz, R., David, J.-P., Rath, M. and Wagner, E.F. 2003. Liver tumor development: C-jun antagonizes the proapoptotic activity of p53. Cell, 112(2): 181-192.
Fialka, I., Schwarz, H., Reichmann, E., Oft, M., Busslinger, M. and Beug, H. 1996. The estrogen-dependent c-juner protein causes a reversible loss of mammary epithelial cell polarity involving a destabilization of adherens junctions. The Journal of cell biology, 132(6): 1115-1132.
Gutzeit, H.O. and Ludwig-M, J. 2014. Plant natural products: Synthesis, biological functions and practical applications. John Wiley & Sons.
Guzowski, J.F., Setlow, B., Wagner, E.K. and McGaugh, J.L. 2001. Experience-dependent gene expression in the rat hippocampus after spatial learning: A comparison of the immediate-early genesarc, c-fos, and zif268. Journal of Neuroscience, 21(14): 5089-5098.
Hanahan, D. 2012. Hallmarks of cancer: A 2012 perspective. Annals of oncology, 23: ix23.
Hartgrink, H.H., Jansen, E.P., van Grieken, N.C. and van de Velde, C.J. 2009. Gastric cancer. The Lancet, 374(9688): 477-490.
Kristiniak, S., Harpel, J., Breckenridge, D.M. and Buckle, J. 2012. Black pepper essential oil to enhance intravenous catheter insertion in patients with poor vein visibility: A controlled study. The Journal of Alternative and Complementary Medicine, 18(11): 1003-1007.
Kwon, H.-K., Hwang, J.-S., So, J.-S., Lee, C.-G., Sahoo, A., Ryu, J.-H., Jeon, W.K., Ko, B.S., Im, C.-R. and Lee, S.H. 2010. Cinnamon extract induces tumor cell death through inhibition of nfκb and ap1. BMC cancer, 10(1): 1-10.
Lieu, C.H., William, W.N. and Lippman, S.M. 2011. Cancer chemoprevention. In: Principles of anticancer drug development. Springer: pp: 463-481.
Lin, L.-T., Wu, S.-J. and Lin, C.-C. 2013. The anticancer properties and apoptosis-inducing mechanisms of cinnamaldehyde and the herbal prescription huang-lian-jie-du-tang (黃連解毒湯 huáng lián jiě dú tang) in human hepatoma cells. Journal of Traditional and Complementary Medicine, 3(4): 227-233.
Long, Y., Wu, Z., Yang, X., Chen, L., Han, Z., Zhang, Y., Liu, J., Liu, W. and Liu, X. 2016. Microrna-101 inhibits the proliferation and invasion of bladder cancer cells via targeting c-fos. Molecular medicine reports, 14(3): 2651-2656.
Malekzadeh, R., Derakhshan, M.H. and Malekzadeh, Z. 2009. Gastric cancer in iran: Epidemiology and risk factors.
Mancini-Filho, J., Van-Koiij, A., Mancini, D., Cozzolino, F. and Torres, R. 1998. Antioxidant activity of cinnamon (cinnamomum zeylanicum, breyne) extracts. Bollettino chimico farmaceutico, 137(11): 443-447.
Milde-Langosch, K. 2005. The fos family of transcription factors and their role in tumourigenesis. European journal of cancer, 41(16): 2449-2461.
Milde‐Langosch, K., Bamberger, A.M., Methner, C., Rieck, G. and Löning, T. 2000. Expression of cell cycle–regulatory proteins rb, p16/mts1, p27/kip1, p21/waf1, cyclin d1 and cyclin e in breast cancer: Correlations with expression of activating protein‐1 family members. International journal of cancer, 87(4): 468-472.
Muhammad, N., Bhattacharya, S., Steele, R., Phillips, N. and Ray, R.B. 2017. Involvement of c-fos in the promotion of cancer stem-like cell properties in head and neck squamous cell carcinoma. Clinical Cancer Research, 23(12): 3120-3128.
Murcia, M.A., Egea, I., Romojaro, F., Parras, P., Jiménez, A.M. and Martínez-Tomé, M. 2004. Antioxidant evaluation in dessert spices compared with common food additives. Influence of irradiation procedure. Journal of Agricultural and Food Chemistry, 52(7): 1872-1881.
Nassr-Allah, A.A., Aboul-Enein, A.M., Aboul-Enein, K.M., Lightfoot, D.A., Cocchetto, A. and El-Shemy, H.A. 2009. Anti-cancer and anti-oxidant activity of some egyptian medicinal plants. Journal of Medicinal Plants Research, 3(10): 799-808.
Nateri, A.S., Spencer-Dene, B. and Behrens, A. 2005. Interaction of phosphorylated c-jun with tcf4 regulates intestinal cancer development. Nature, 437(7056): 281-285.
Ranasinghe, P., Pigera, S., Premakumara, G., Galappaththy, P., Constantine, G.R. and Katulanda, P. 2013. Medicinal properties of ‘true’cinnamon (cinnamomum zeylanicum): A systematic review. BMC complementary and alternative medicine, 13(1): 1-10.
Sadeghi, S., Davoodvandi, A., Pourhanifeh, M.H., Sharifi, N., ArefNezhad, R., Sahebnasagh, R., Moghadam, S.A., Sahebkar, A. and Mirzaei, H. 2019. Anti-cancer effects of cinnamon: Insights into its apoptosis effects. European journal of medicinal chemistry, 178: 131-140.
Soussi, T. 2000. P53 antibodies in the sera of patients with various types of cancer: A review. Cancer research, 60(7): 1777-1788.
Ukiya, M., Akihisa, T., Yasukawa, K., Tokuda, H., Suzuki, T. and Kimura, Y. 2006. Anti-inflammatory, anti-tumor-promoting, and cytotoxic activities of constituents of marigold (calendula officinalis) flowers. Journal of natural products, 69(12): 1692-1696.
Venkatachalam, K., Vinayagam, R., Arokia Vijaya Anand, M., Isa, N.M. and Ponnaiyan, R. 2020. Biochemical and molecular aspects of 1, 2-dimethylhydrazine (dmh)-induced colon carcinogenesis: A review. Toxicology research, 9(1): 2-18.
Vleugel, M.M., Greijer, A.E., Bos, R., van der Wall, E. and van Diest, P.J. 2006. C-jun activation is associated with proliferation and angiogenesis in invasive breast cancer. Human pathology, 37(6): 668-674.
Vogt, P.K. 2012. Retroviral oncogenes: A historical primer. Nature Reviews Cancer, 12(9): 639-648.
Yue, X., Zhao, Y., Xu, Y., Zheng, M., Feng, Z. and Hu, W. 2017. Mutant p53 in cancer: Accumulation, gain-of-function, and therapy. Journal of molecular biology, 429(11): 1595-1606.
Zaker Bostanabad, S., Darmanlo, S. and Darmanloo, S. 2020. Evaluation of vegfa and mthfd1 polymorphism in the blood cells of iranian women with recurrent miscarriage. Research in Karyotic Cell & Tissue, 1(1): 9-15.