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بررسی تاثیر بوسولفان بر تغییرات بیان ژن‌های Nrf -2 و Keap-1 در بافت بیضه رت‌های نر نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده داروسازی و علوم دارویی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه علوم پایه، دانشکده داروسازی و علوم دارویی، علوم پزشکی تهران،دانشگاه آزاد اسلامی،تهران،ایران

3 گروه زیست شناسی، دانشکده علوم پایه، واحد همدان، دانشگاه آزاد اسلامی، همدان، ایران

10.30495/rkctc.2022.65731.1035

چکیده

پیشینه مطالعه و هدف: یکی از شناخته ترین عوارض داروهای ضد سرطان ایجاد اختلال در روند اسپرماتوژنز است که در موارد زیادی ایجاد ناباروری می‌گردد. هدف بررسی حاضر ارزیابی تغییر بیان ژن های Nrf-2 و Keap-1 در اثر تزریق صفاقی بوسولفان در موش‌های نر نژاد ویستار انجام گردید.
روش مطالعه: در این مطالعه تعداد 20 رت نر ویستار آلبینو بالغ دوماهه به وزن تقریبی 200-150 گرم در دو گروه کنترل (سالم) و گروه دریافت کننده بوسولفان وارد مطالعه شدند. پس از گذشت دوره مد نظر بافت بیضه موش‌ها جداسازی شد به لحاظ بیان ژن های Nrf-2 و Keap-1 و همچنین تغییرات هورمون های تستوسترون، FSH و LH مورد ارزیابی قرار گرفتند. داده­ها با نرم افزارSPSS وآزمون آماری One-way ANOVA مورد تجزیه تحلیل قرار گرفت.
نتایج: بررسی آماری نتایج حاصله نشان داد بیان ژن Nrf-2 و Keap-1 در گروه تیمار (بوسولفان) نسبت به گروه شاهد کاهش پیدا کرده است اما این کاهش به لحاظ آماری معنادار نبود (0.2951 و P=0.3528). سطح هورمون LH و تستوسترون بین دو گروه کنترل و مورد درمان تفاوت معناداری نداشت (0.1327 و P =0.0809) اما سطح هورمون FSH بین دو گروه معنادار (P =0.0189) بود.
نتیجه گیری: نتایج این پژوهش نشان داد که بوسولفان می‌تواند با تغییراتی که در بیان ژن‌های keap1 و Nrf2 داشته باشد، سطح هورمون FSH که در فرآیند اسپرماتوژنز موثر است را تحت تاثیر قرار دهد و موجب ناباروری گردد. لذا از این فاکتورهای ژنی و هورمونی می‌توان جهت پیش آگاهی ناباروری استفاده نمود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of busulfan on the expression changes of Nrf -2 and Keap-1 genes in testicular tissue of male Wistar rats

نویسندگان [English]

  • sasan Kalantari 1
  • Tahereh Naji 2
  • Rahim Ahmadi 3

1 Pharmacy Student, Department of Basic Sciences, Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

2 Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

3 Department of Biology, Faculty of Basic Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran

چکیده [English]

Introduction and Aim:  One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar rats.
Methods: In this study, 20 two-month-old adult male Wistar albino rats weighing approximately 200-150 g were studied in two groups: the control group (healthy) and the busulfan group. After this period, the testicular tissue of the mice was isolated and evaluated for the expression of Nrf-2 and Keap-1 genes, as well as changes in testosterone, FSH, and LH. Data were analyzed using SPSS software and a one-way ANOVA statistical test.
Results: The statistical analysis of the results showed that the expression of Nrf-2 and Keap-1 genes in the treatment group (busulfan) decreased compared to the control group, but this decrease was not statistically significant (0.2951 and P = 0.3528). LH and testosterone levels were not significantly different between the control and treated groups (0.1327 and P = 0.0809) but FSH levels were significant between the two groups (P = 0.0189).
Conclusion: The results of this study showed that busulfan can affect the level of FSH, which is effective in spermatogenesis, by causing changes in the expression of keap1 and Nrf-2 genes and causing infertility. Therefore, these genetic and hormonal factors can be used to predict infertility.

کلیدواژه‌ها [English]

  • Infertility
  • Busulfan
  • Oxidative stress
  • Gene expression changes
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پژوهش‌هایی در یاخته و بافت
دوره 2، شماره 3
مهر 1400
صفحه 12-17
فایل ها
اشتراک گذاری
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