نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار پژوهش، موسسه تحقیقات جنگل‌ها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

2 گروه زیست شناسی دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران ایران

چکیده

پیشینه و هدف: این تحقیق به منظور شناخت پاسخ های آناتومیک گیاه بادرنجبویه به نانوسلنیوم (nSe) انجام شد.
روش بررسی: این تحقیق nSe در غلظت های ۱۰ و 5۰ میلی‌گرم در لیتر انجام شد.
نتایج: نتایج این تحقیق دلالت بر آن داشت که نانوسلنیوم در غلظت 10 اثرات مثبت و مفیدی داشت اما غلظت 50 موجب القا سمیت شد. ارزیابی میکروسکوپی مقاطع ساقه نشان داد که قطر استوانه مرکزی نسبت به شاهد افزایش یافت. بیشترین افزایش مربوط به دانه رست های در معرض دوزهای 50 و 10 میلی گرم در لیتر سلنیوم بود. ضخامت و قطر ساقه در نمونه‌های تیمار شده با nSe در غلظت 50 میلی گرم در لیتر و بالک 10 میلی گرم در لیتر نسبت به شاهد افزایش یافت. قطر آوند چوبی در پاسخ به مکمل ها کاهش یافت و کمترین میزان متعلق به گروه nSe50 بود. بررسی سطح مقطع برگ نشان داد که nSe10 باعث افزایش ضخامت قطر آوند چوبی، پرده و پارانشیم اسفنجی نسبت به شاهد شد. مقایسه سطح مقطع دمبرگ نشان داد که ضخامت اپیدرم و قطر آوند چوبی مرکزی و جانبی تغییر کرده است. در حالی که 50 میلی گرم در لیتر nSe این صفات را کاهش داد.
نتیجه‌گیری: نتایج این مطالعه حاکی از آن است که کاربرد سلنیوم وابسته به غلظت، علاوه بر القای تغییرات فیزیولوژیکی و مولکولی، موجب تغییرات آناتومیکی و تمایز بافتی می‌شود. تحقیقات بیشتری در آینده برای تعیین مکانیسم این پاسخ ها مورد نیاز است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Differential tissue differentiation in lemon balm following the application of selenium nanoparticles

نویسندگان [English]

  • Somayyeh Kheiri 1
  • Alameh Babajani 2

1 Assistant professor, Research institute of forests and Rangelands, agricultural research, education and extension organization (AREEO), Tehran, Iran

2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Background and aim: This study was performed to identify the anatomical responses of lemon balm to selenium nanoparticles (nSe).
Methods: This experimental laboratory study was done at the concentrations of 10 and 50 mg/L of nSe.
Results: Plants were treated with nano-selenium (nSe; 0, 10 and 50 mg/l) and bulk selenium (BSe). The results of this study indicated that nano selenium had a positive and beneficial effect at a concentration of 10, but a concentration of 50 induced toxicity. The microscopic assessment of stem cross-sections showed that the diameter of the central cylinder was increased compared to the control. The highest increase was related to the seedlings exposed to the 50 and 10 mg/l. Stem thickness and diameter in the nSe-treated seedlings at 50 mg/l and bulk at 10 mg/l were increased compared to the control. Xylem diameter decreased in response to the supplements, the lowest belongs to the nSe50. Monitoring the leaf cross-sections showed that nSe10 increased the thickness of xylem diameter, palisade, and sponge parenchyma compared to the control. A comparison of petiole cross-sections indicated that the thickness of the epidermis and the diameter of the central and lateral xylem were changed. While 50 mg/l nSe declined these traits.
Conclusion: Therefore, the results of this study indicate that the application of applied concentration-dependent selenium, in addition to inducing physiological and molecular changes, causes anatomical changes and tissue differentiation. Further research is needed in the future to determine the mechanism of these responses.

کلیدواژه‌ها [English]

  • Nanomaterials
  • Selenium
  • Lemon balm
  • Anatomy
  • Tissue
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