نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیستشناسی، دانشگاه شهیدمدنی آذربایجان، تبریز، ایران

2 گروه زیست شناسی، دانشگاه آزاد اسلامی واحد گرمسار، گرمسار، ایران

10.30495/rkctc.2022.67085.1042

چکیده

To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases in root and shoot fresh weights, and chlorophyll content. While, the nSe at 50 mgl-1 exhibited severe phyotoxicity. Also, nSe10 enhanced uptake and accumulation of Ca and Mg in both leaf and root, contrasted to the nSe50-treated plants. The applied supplements modified phenylalanine ammonia lyase activity, concentrations of flavonids, glutathione, and proline. Moreover, these supplements in the dose and type-dependent manners changed the activities of catalase. Furthermore, the applied treatments up-regulated the expression of phenylalanine ammonia-lyase (PAL) and Coumarate: CoA-ligase (4CL) genes. The comparative physiological and molecular evidence on phytotoxicity and potential advantages of nSe and its bulk counterpart was provided as a theoretical basis for exploiting in food, agricultural, and pharmaceutical industries.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Elemental selenium nanoparticles contributed to changes in primary and secondary metabolism in Lemon balm by affecting gene expression and cellular redox status

نویسندگان [English]

  • Leila Zarandi-Miandoab 1
  • Zahra Oraghi Ardebili 2

1 Department of Plant Biology, Azarbaijan University of Tarbiat Moallem, Tabriz, Iran

2 Department of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Iran

چکیده [English]

To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases in root and shoot fresh weights, and chlorophyll content. While, the nSe at 50 mgl-1 exhibited severe phyotoxicity. Also, nSe10 enhanced uptake and accumulation of Ca and Mg in both leaf and root, contrasted to the nSe50-treated plants. The applied supplements modified phenylalanine ammonia lyase activity, concentrations of flavonids, glutathione, and proline. Moreover, these supplements in the dose and type-dependent manners changed the activities of catalase. Furthermore, the applied treatments up-regulated the expression of phenylalanine ammonia-lyase (PAL) and Coumarate: CoA-ligase (4CL) genes. The comparative physiological and molecular evidence on phytotoxicity and potential advantages of nSe and its bulk counterpart was provided as a theoretical basis for exploiting in food, agricultural, and pharmaceutical industries.

کلیدواژه‌ها [English]

  • Biofortification
  • Gene expression
  • Nanoparticles
  • Selenium
  • Toxicity

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019a. Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles. Environmental Science and Pollution Research 26, 24430–24444.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019b. Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles, cold plasma as a novel emerging tool for plant science. Plasma Chem Plasma Process 391, 21-34.

Bates, L.S., Walrow, R.P., Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant Soil 39, 205–208

Beaudoin-Eagan, L.,D., Thorpe, T.A., 1985. Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures. Plant Physiology 78, 438–441

Djanaguiraman, M., Belliraj, N., Bossmann, S.,H., Prasad, P.,V. 2018. High-Temperature Stress Alleviation by Selenium Nanoparticle Treatment in Grain Sorghum. ACS Omega 33, 2479-2491

Djanaguiraman, M., Prasad, P.,V., Seppanen, M. 2010. Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system. Plant physiology and Biochemistry 4812, 999-1007

Geneva, M., Hristozkova, M., Yonova, P., Boychinova, M., Stancheva, I. 2010. Effect of endomycorrhizal colonization with Glomus intraradices on growth and antioxidant capacity of Sideritis scardica Griseb. General Appl Plant Physiol 362, 47-54

Hussein, H.A.A., Darwesh, O.M. and Mekki, B.B., 2019. Environmentally friendly nano-selenium to improve antioxidant system and growth of groundnut cultivars under sandy soil conditions. Biocatalysis and agricultural biotechnology, 18, 101080.

Hu, T., Li, H., Li, J., Zhao, G., Wu, W., Liu, L., Wang, Q., Guo, Y. 2018. Absorption and bio-transformation of Selenium nanoparticles by wheat seedlings Triticum aestivum L. Front Plant Sci 9, 597

Iranbakhsh, A., Ardebili, Z.O., Ardebili, N.O., Ghoranneviss, M., Safari, N. 2018. Cold plasma relieved toxicity signs of nano zinc oxide in Capsicum annuum cayenne via modifying growth differentiation and physiology. Acta Physiol Plant 408, 154

Lichtenthaler, H., Wellburn, A. 1983. Determination of totalcarotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 603, 591–592

Lima, L.W., Pilon-Smits, E.A., Schiavon, M. 2018. Mechanisms of selenium hyperaccumulation in plants, A survey of molecular biochemical and ecological cues. Biochim Biophys Acta 186211, 2343-53

Malandrakis, A.A., Kavroulakis, N., Chrysikopoulos, C.V. 2019. Use of copper silver and zinc nanoparticles against foliar and soil-borne plant pathogens. Sci Total Environ 670, 292-299

Moghaddasi, S., Fotovat, A., Khoshgoftarmanesh, A.H., Karimzadeh, F., Khazaei, H.R., Khorassani, R. 2017. Bioavailability of coated and uncoated ZnO nanoparticles to cucumber in soil with or without organic matter. Ecotoxicol Environ Saf 144, 543-551

Moghanloo, M., Iranbakhsh, A., Ebadi, M., Ardebili, Z.O. 2019a. Differential physiology and expression of phenylalanine ammonia lyase PAL and universal stress protein USP. in the endangered species Astragalus fridae following seed priming with cold plasma and manipulation of culture medium with silica nanoparticles. 3 Biotech 97, 288.

Moghanloo, M., Iranbakhsh, A., Ebadi, M., Satari, T.N., Ardebili, Z.O. 2019b. Seed priming with cold plasma and supplementation of culture medium with silicon nanoparticle modified growth physiology and anatomy in Astragalus fridae as an endangered species. Acta Physiol Plant 414., 54

Nadgórska-Socha, A., Kafel, A., Kandziora-Ciupa, M., Gospodarek, J., Zawisza-Raszka, A. 2013. Accumulation of heavy metals and antioxidant responses in Vicia faba plants grown on monometallic contaminated soil. Environ Sci Poll Res 202, 1124-1134

Nazerieh, H., Oraghi Ardebili. Z., Iranbakhsh, A. 2018. Potential benefits and toxicity of nanoselenium and nitric oxide in peppermint. Acta Agric Slov 1112., 357-368

Oraghi Ardebili, Z., Ardebili, N.O., Jalili, S., Safiallah, S. 2015. The modified qualities of basil plants by selenium and/or ascorbic acid. Turk J Bot 393, 401-407

Pereira, A.S., Dorneles, A.O.S., Bernardy, K., Sasso, M., Bernardy, D., Possebom, G., Rossato, L.V., Dressler, V.L., Tabaldi, L.A. 2018. Selenium and silicon reduce cadmium uptake and mitigate cadmium toxicity in Pfaffia glomerata Spreng. Pedersen plants by activation antioxidant enzyme system. Environ Sci Poll Res 2519., pp.18548-18558

Puccinelli, M., Malorgio, F., Rosellini, I., Pezzarossa, B. 2017. Uptake and partitioning of selenium in basil Ocimum basilicum L. plants grown in hydroponics. Sci Hortic 225, 271-276

Rawat, S., Adisa, I.O., Wang, Y., Sun, Y., Fadil, A.S., Niu, G., Sharma, N., Hernandez-Viezcas, J.A., Peralta-Videa, J.R., Gardea-Torresdey, J.L. 2019. Differential physiological and biochemical impacts of nano vs micron Cu at two phenological growth stages in bell pepper Capsicum annuum. plant. Nano Impact 14, 100161

Rizwan, M., Ali, S., Ali, B., Adrees, M., Arshad, M., Hussain, A., Rehman, M.Z., Waris, A.A. 2019. Zinc and iron oxide nanoparticles improved the plant growth and reduced the oxidative stress and cadmium concentration in wheat. Chemosphere 214, 269-277

Safari, M., Ardebili, Z.O., Iranbakhsh, A. 2018. Selenium nano-particle induced alterations in expression patterns of heat shock factor A4A HSFA4A. and high molecular weight glutenin subunit 1Bx Glu-1Bx. and enhanced nitrate reductase activity in wheat Triticum aestivum L. Acta Physiol Plant 406, 117

Seddighinia, F.S., Iranbakhsh, A., Ardebili, Z.O., Satari, T.N., Soleimanpour, S. 2019. Seed priming with cold plasma and multi-walled carbon nanotubes modified growth, tissue differentiation, anatomy, and yield in bitter melon Momordica charantia. J Plant Growth Regul https,//doi.org/10.1007/s00344-019-09965-2

Wang, H., Zhang, J., Yu, H. 2007. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes, comparison with selenomethionine in mice. Free Radical Biol 4210,1524-33.

Wu, Z., Liu, S., Zhao, J., Wang, F., Du, Y., Zou, S., Li, H., Wen, D., Huang, Y. 2017. Comparative responses to silicon and selenium in relation to antioxidant enzyme system and the glutathione-ascorbate cycle in flowering Chinese cabbage Brassica campestris L ssp chinensis var utilis under cadmium stress. Environ Exp Bot 133, 1-11

Zhong, C., Cao, X., Bai, Z., Zhang, J., Zhu, L., Huang, J., Jin, Q. 2018. Nitrogen metabolism correlates with the acclimation of photosynthesis to short-term water stress in rice Oryza sativa L. Plant physiology and Biochemistry 125, 52-62