اثر نانو ذره و بالک اکسید روی بر الگوی تمایز بافتی در گیاه بادرنجبویه (Melissa officinalis)

خیری, سمیه; باباجانی, عالمه

doi:10.30495/rkctc.2022.67083.1040

نوع مقاله : مقاله پژوهشی

نویسندگان

¹ استادیار پژوهش، موسسه تحقیقات جنگل‌ها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

² گروه زیست شناسی دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران ایران

10.30495/rkctc.2022.67083.1040

چکیده

پیشینه و هدف: این تحقیق به منظور شناخت پاسخ های آناتومیک گیاه بادرنجبویه به نانواکسید روی (nZnO) انجام شد.
روش بررسی: این تحقیق nZnO در غلظت های ۱۰۰ و ۳۰۰ میلی‌گرم در لیتر انجام شد.
نتایج: نتایج حاصل از بررسی های میکروسکوپی نشان داد که گروه nZnO300 بیشترین قطر استوانه مرکزی و بیشترین قطر زایلم و قطر فلوئم را در ریشه نسبت به شاهد داشتند. بررسی برش عرضی ساقه نشان داد که بیشترین قطر ساقه در گروه nZnO100 مشاهده شد. بیشترین ضخامت کلانشیم و قطر استوانه مرکزی و قطر فلوئم و زایلم نیز درnZnO100 ثبت شد. از مقایسه برش های عرضی برگ های تیمارها ملاحظه شد که ضخامت اپیدرم بالایی، ضخامت اپیدرم پایینی، قطر زایلم، ضخامت پارانشیم نرده ای و اسفنجی درگروه nZnO100 نسبت به شاهد افزایش معنی دار داشت. مقایسه برش های عرضی دمبرگ مشخص کرد که ضخامت اپیدرم بالایی و پایینی و قطر فلوئم و زایلم مرکزی و ضخامت فلوئم و زایلم جانبی در تمامی گروه های تیماری نسبت به شاهد کاهش معنی دار داشته است.
نتیجه‌گیری: نتایج این تحقیق شواهد آناتومیک را ارایه نمود که بیانگر تغییرات اساسی در سرنوشت تمایز سلول‌ها و تشکیل بافت ها دارد. تحقیقات آینده برای برقراری ارتباط بین شواهد فیزیولوژیک، مولکولی و آناتومیک برای شناخت دقیق تاثیر محصولات نانو بر کل زندگی گیاهان به ویژه گیاهان زراعی و دارویی ضرورت دارد.

کلیدواژه‌ها

موضوعات

کشت سلول و بافت 'گیاهی

عنوان مقاله [English]

Effects of bulk and nanoparticles of zinc oxide on the tissue differentiation in Melissa officinalis

نویسندگان [English]

Somayyeh Kheiri ¹
Alameh Babajani ²

² Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده [English]

Background and aim: This study was performed to identify the anatomical responses of lemon balm to zinc oxide nanoparticles (nZnO).
Methods: This experimental laboratory study was done at the concentrations of 100 and 300 mg/L of nZnO.
Results: The microscopic observation showed that the nZnO300 had the largest diameter of the central cylinder and the diameter of xylem and phloem in the root compared to the control. The study of stem cross-section revealed that the highest stem diameter was observed in response to the nZnO100 group. The maximum thickness of collenchyma and the highest diameter of the central cylinder and the diameter of phloem and xylem were also recorded in the nZnO100 group. Comparing the leaf cross-sections indicated that the thickness of the upper and lower epidermis, the diameter of the xylem, and the thickness of the spongy parenchyma in the nZnO100 group were significantly increased relative to the control. A comparison of petiole cross-sections showed that the thickness of the upper and lower epidermis and the diameter of the central/lateral phloem and xylem in all nZnO-treated seedlings were significantly reduced compared to the control.
Conclusion: This study provided anatomical evidence that indicates fundamental changes in the fate of cell differentiation and tissue formation in response to nZnO. Future research is needed to link physiological, molecular, and anatomical evidence to accurately understand the impact of nanoproducts on the entire life of plants, especially crops and medicines.

کلیدواژه‌ها [English]

Nanomaterials
Zinc oxide
Lemon balm
Anatomy
Tissue

مراجع

Adrees, M., Khan, Z.S., Hafeez, M., Rizwan, M., Hussain, K., Asrar, M., Alyemeni, M.N., Wijaya, L. and Ali, S., 2021. Foliar exposure of zinc oxide nanoparticles improved the growth of wheat (Triticum aestivum L.) and decreased cadmium concentration in grains under simultaneous Cd and water deficient stress. Ecotoxicology and Environmental Safety, 208, p.111627.

Asgari-Targhi, G., Iranbakhsh, A., Ardebili, Z.O. and Tooski, A.H., 2021. Synthesis and characterization of chitosan encapsulated zinc oxide (ZnO) nanocomposite and its biological assessment in pepper (Capsicum annuum) as an elicitor for in vitro tissue culture applications. International Journal of Biological Macromolecules, 189, pp.170-182.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019^a. Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019^b. Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles, cold plasma as a novel emerging tool for plant science. Plasma Chem Plasma Process 391, 21-34.

Faizan, M., Bhat, J.A., Chen, C., Alyemeni, M.N., Wijaya, L., Ahmad, P. and Yu, F., 2021. Zinc oxide nanoparticles (ZnO-NPs) induce salt tolerance by improving the antioxidant system and photosynthetic machinery in tomato. Plant Physiology and Biochemistry, 161, pp.122-130.

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Mirakhorli T, Ardebili ZO, Ladan-Moghadam A, Danaee E (2021) Bulk and nanoparticles of zinc oxide exerted their beneficial effects by conferring modifications in transcription factors, histone deacetylase, carbon and nitrogen assimilation, antioxidant biomarkers, and secondary metabolism in soybean. PLoS ONE 16(9): e0256905.

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Rajaee Behbahani S, Iranbakhsh A, Ebadi M, Majd A, Ardebili ZO (2020) Red elemental selenium nanoparticles mediated substantial variations in growth, tissue differentiation, metabolism, gene transcription, epigenetic cytosine DNA methylation, and callogenesis in bittermelon (Momordica charantia); an in vitro experiment. PLoS ONE 15(7): e0235556.

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Sheteiwy, M.S., Dong, Q., An, J., Song, W., Guan, Y., He, F., Huang, Y., Hu, J. 2017. Regulation of ZnO nanoparticles-induced physiological and molecular changes by seed priming with humic acid in Oryza sativa seedlings. Plant Growth Regul 831., 27-41

Sotoodehnia-Korani, S., Iranbakhsh, A., Ebadi, M., Majd, A. and Ardebili, Z.O., 2020. Selenium nanoparticles induced variations in growth, morphology, anatomy, biochemistry, gene expression, and epigenetic DNA methylation in Capsicum annuum; an in vitro study. Environmental Pollution, 265, p.114727.

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Tripathi, D.K., Mishra, R.K., Singh, S., Singh, S., Singh, V.P., Singh, P.K., Chauhan, D.K., Prasad, S.M., Dubey, N.K. and Pandey, A.C., 2017. Nitric oxide ameliorates zinc oxide nanoparticles phytotoxicity in wheat seedlings:

پژوهش‌هایی در یاخته و بافت

اثر نانو ذره و بالک اکسید روی بر الگوی تمایز بافتی در گیاه بادرنجبویه (Melissa officinalis)

مراجع

مراجع

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019^a. Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019^b. Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles, cold plasma as a novel emerging tool for plant science. Plasma Chem Plasma Process 391, 21-34.

Sheteiwy, M.S., Dong, Q., An, J., Song, W., Guan, Y., He, F., Huang, Y., Hu, J. 2017. Regulation of ZnO nanoparticles-induced physiological and molecular changes by seed priming with humic acid in Oryza sativa seedlings. Plant Growth Regul 831., 27-41

Sotoodehnia-Korani, S., Iranbakhsh, A., Ebadi, M., Majd, A. and Ardebili, Z.O., 2020. Selenium nanoparticles induced variations in growth, morphology, anatomy, biochemistry, gene expression, and epigenetic DNA methylation in Capsicum annuum; an in vitro study. Environmental Pollution, 265, p.114727.

دوره 2، شماره 4
اسفند 1400
صفحه 1-6

اثر نانو ذره و بالک اکسید روی بر الگوی تمایز بافتی در گیاه بادرنجبویه (Melissa officinalis)

مراجع

مراجع

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019a. Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019b. Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles, cold plasma as a novel emerging tool for plant science. Plasma Chem Plasma Process 391, 21-34.

Sheteiwy, M.S., Dong, Q., An, J., Song, W., Guan, Y., He, F., Huang, Y., Hu, J. 2017. Regulation of ZnO nanoparticles-induced physiological and molecular changes by seed priming with humic acid in Oryza sativa seedlings. Plant Growth Regul 831., 27-41

دوره 2، شماره 4اسفند 1400صفحه 1-6

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019^a. Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019^b. Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles, cold plasma as a novel emerging tool for plant science. Plasma Chem Plasma Process 391, 21-34.

دوره 2، شماره 4
اسفند 1400
صفحه 1-6