Document Type : Original Article

Authors

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Background and aim: This study was performed to identify the anatomical responses of lemon balm to zinc oxide nanoparticles (nZnO).
Methods: This experimental laboratory study was done at the concentrations of 100 and 300 mg/L of nZnO.
Results: The microscopic observation showed that the nZnO300 had the largest diameter of the central cylinder and the diameter of xylem and phloem in the root compared to the control. The study of stem cross-section revealed that the highest stem diameter was observed in response to the nZnO100 group. The maximum thickness of collenchyma and the highest diameter of the central cylinder and the diameter of phloem and xylem were also recorded in the nZnO100 group. Comparing the leaf cross-sections indicated that the thickness of the upper and lower epidermis, the diameter of the xylem, and the thickness of the spongy parenchyma in the nZnO100 group were significantly increased relative to the control. A comparison of petiole cross-sections showed that the thickness of the upper and lower epidermis and the diameter of the central/lateral phloem and xylem in all nZnO-treated seedlings were significantly reduced compared to the control.
Conclusion: This study provided anatomical evidence that indicates fundamental changes in the fate of cell differentiation and tissue formation in response to nZnO. Future research is needed to link physiological, molecular, and anatomical evidence to accurately understand the impact of nanoproducts on the entire life of plants, especially crops and medicines.

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Main Subjects

Adrees, M., Khan, Z.S., Hafeez, M., Rizwan, M., Hussain, K., Asrar, M., Alyemeni, M.N., Wijaya, L. and Ali, S., 2021. Foliar exposure of zinc oxide nanoparticles improved the growth of wheat (Triticum aestivum L.) and decreased cadmium concentration in grains under simultaneous Cd and water deficient stress. Ecotoxicology and Environmental Safety, 208, p.111627.
Asgari-Targhi, G., Iranbakhsh, A., Ardebili, Z.O. and Tooski, A.H., 2021. Synthesis and characterization of chitosan encapsulated zinc oxide (ZnO) nanocomposite and its biological assessment in pepper (Capsicum annuum) as an elicitor for in vitro tissue culture applications. International Journal of Biological Macromolecules, 189, pp.170-182.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019a. Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles.

Babajani, A., Iranbakhsh, A., Ardebili, Z.,O., Eslami, B. 2019b. Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles, cold plasma as a novel emerging tool for plant science. Plasma Chem Plasma Process 391, 21-34.

Faizan, M., Bhat, J.A., Chen, C., Alyemeni, M.N., Wijaya, L., Ahmad, P. and Yu, F., 2021. Zinc oxide nanoparticles (ZnO-NPs) induce salt tolerance by improving the antioxidant system and photosynthetic machinery in tomato. Plant Physiology and Biochemistry, 161, pp.122-130.
Hussain A, Ali S, Rizwan M, Rehman MZ, Javed MR, Imran M et al. Zinc oxide nanoparticles alter the wheat physiological response and reduce the cadmium uptake by plants. Environ Pollut. 2018; 242: 1518-1526.
Mirakhorli T, Ardebili ZO, Ladan-Moghadam A, Danaee E (2021) Bulk and nanoparticles of zinc oxide exerted their beneficial effects by conferring modifications in transcription factors, histone deacetylase, carbon and nitrogen assimilation, antioxidant biomarkers, and secondary metabolism in soybean. PLoS ONE 16(9): e0256905.
Plaksenkova, I., Kokina, I., Petrova, A., Jermaļonoka, M., Gerbreders, V. and Krasovska, M., 2020. The Impact of Zinc Oxide Nanoparticles on Cytotoxicity, Genotoxicity, and miRNA Expression in Barley (Hordeum vulgare L.) Seedlings. The Scientific World Journal.
Rajaee Behbahani S, Iranbakhsh A, Ebadi M, Majd A, Ardebili ZO (2020) Red elemental selenium nanoparticles mediated substantial variations in growth, tissue differentiation, metabolism, gene transcription, epigenetic cytosine DNA methylation, and callogenesis in bittermelon (Momordica charantia); an in vitro experiment. PLoS ONE 15(7): e0235556.
Semida, W.M., Abdelkhalik, A., Mohamed, G., El-Mageed, A., Taia, A., El-Mageed, A., Shimaa, A., Rady, M.M. and Ali, E.F., 2021. Foliar application of zinc oxide nanoparticles promotes drought stress tolerance in eggplant (Solanum melongena L.). Plants, 10(2), p.421.

Sheteiwy, M.S., Dong, Q., An, J., Song, W., Guan, Y., He, F., Huang, Y., Hu, J. 2017. Regulation of ZnO nanoparticles-induced physiological and molecular changes by seed priming with humic acid in Oryza sativa seedlings. Plant Growth Regul 831., 27-41

Sotoodehnia-Korani, S., Iranbakhsh, A., Ebadi, M., Majd, A. and Ardebili, Z.O., 2020. Selenium nanoparticles induced variations in growth, morphology, anatomy, biochemistry, gene expression, and epigenetic DNA methylation in Capsicum annuum; an in vitro study. Environmental Pollution, 265, p.114727.

Sun L, Wang Y, Wang R, Wang R, Zhang P, Ju Q, Xu J. Physiological, transcriptomic, and metabolomic analyses reveal zinc oxide nanoparticles modulate plant growth in tomato. Environ Sci Nano. 2020; 7(11): 3587-3604.
Tripathi, D.K., Mishra, R.K., Singh, S., Singh, S., Singh, V.P., Singh, P.K., Chauhan, D.K., Prasad, S.M., Dubey, N.K. and Pandey, A.C., 2017. Nitric oxide ameliorates zinc oxide nanoparticles phytotoxicity in wheat seedlings: