Comparison of the effect of different sources of cold plasma production on the growth of MCF-7 cell line

Hekmat, Azadeh; Zahedian, Setareh

doi:10.30495/rkctc.2022.71088.1048

Document Type : Original Article

Authors

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

10.30495/rkctc.2022.71088.1048

Abstract

Introduction and aim: Cold atmospheric plasma (CAP) is a new anti-cancer therapeutic method in the field of plasma medicine, with high selectivity to kill cancer cells more than normal cells. In recent years plasma-activated solutions, specifically plasma-activated medium (PAM) have also shown their selective anti-cancer effect, like direct CAP treatment. Different sources produce plasma, and the purpose of this research was to investigate the effect of various plasma production devices on the growth of MCF-7 breast cancer cells.
Methods: The effect of PAM produced from three different sources of cold atmospheric plasma on the human breast cancer cell line (MCF-7) was compared using the MTT test.
Results: The cells treated with PAM of the Parla plasma jet in 1, 2, 3, and 4 minutes decreased the viability of the cells by 35%, respectively. The cells treated with PAM of electric charge discharge plasma device caused significant cell death, but the culture cells treated with PAM of argon plasma jet device did not affect cell survival.
Conclusion: The present findings showed that the type of cold atmospheric plasma source is effective on cell survival rate.

Keywords

Main Subjects

Biophysics and Cancer

References

Adachi, T., Tanaka, H., Nonomura, S., Hara, H., Kondo, S.-i. and Hori, M. 2015. Plasma-activated medium induces a549 cell injury via a spiral apoptotic cascade involving the mitochondrial–nuclear network. Free Radical Biology and Medicine, 79: 28-44.

Azzariti, A., Iacobazzi, R.M., Di Fonte, R., Porcelli, L., Gristina, R., Favia, P., Fracassi, F., Trizio, I., Silvestris, N. and Guida, G. 2019. Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells. Scientific reports, 9(1): 4099.

Gay-Mimbrera, J., Garcia, M.C., Isla-Tejera, B., Rodero-Serrano, A., Garcia-Nieto, A.V. and Ruano, J. 2016. Clinical and biological principles of cold atmospheric plasma application in skin cancer. Adv Ther, 33(6): 894-909.

Hekmat, A., Hatamie, S. and Saboury, A.A. 2022. The effects of synthesized silver nanowires on the structure and esterase-like activity of human serum albumin and their impacts on human endometrial stem cells. Inorganic and Nano-Metal Chemistry: 1-14.

Karki, S.B., Gupta, T.T., Yildirim-Ayan, E., Eisenmann, K.M. and Ayan, H. 2017. Investigation of non-thermal plasma effects on lung cancer cells within 3d collagen matrices. Journal of Physics D: Applied Physics, 50(31): 315401.

Karki, S.B., Yildirim-Ayan, E., Eisenmann, K.M. and Ayan, H. 2017. Miniature dielectric barrier discharge nonthermal plasma induces apoptosis in lung cancer cells and inhibits cell migration. BioMed research international, 2017.

Kumar, N., Attri, P., Dewilde, S. and Bogaerts, A. 2018. Inactivation of human pancreatic ductal adenocarcinoma with atmospheric plasma treated media and water: A comparative study. Journal of Physics D: Applied Physics, 51(25): 255401.

Kurake, N., Ishikawa, K., Tanaka, H., Hashizume, H., Nakamura, K., Kajiyama, H., Toyokuni, S., Kikkawa, F., Mizuno, M. and Hori, M. 2019. Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of u251sp glioblastoma. Archives of biochemistry and biophysics, 662: 83-92.

Rhmanian, N., Hekmat, A. and Hajebrahimi, Z. 2022. Effect of simulated microgravity condition on cells proliferation and myostatin gene expression in differentiated skeletal muscle cells (c2c12). Journal of Space Science and Technology, 15(2): -. DOI 10.30699/jsst.2021.288166.1346.

Schneider, C., Gebhardt, L., Arndt, S., Karrer, S., Zimmermann, J.L., Fischer, M.J. and Bosserhoff, A.-K. 2018. Cold atmospheric plasma causes a calcium influx in melanoma cells triggering cap-induced senescence. Scientific reports, 8(1): 10048.

Sivachandiran, L. and Khacef, A. 2017. Enhanced seed germination and plant growth by atmospheric pressure cold air plasma: Combined effect of seed and water treatment. RSC Advances, 7(4): 1822-1832.

Tendero, C., Tixier, C., Tristant, P., Desmaison, J. and Leprince, P. 2006. Atmospheric pressure plasmas: A review. Spectrochimica Acta Part B: Atomic Spectroscopy, 61(1): 2-30.

Yan, D., Sherman, J.H. and Keidar, M. 2017. Cold atmospheric plasma, a novel promising anti-cancer treatment modality. Oncotarget, 8(9): 15977.

Zahedian, S., Hekmat, A., Tackallou, S.H. and Ghoranneviss, M. 2022. The impacts of prepared plasma-activated medium (pam) combined with doxorubicin on the viability of mcf-7 breast cancer cells: A new cancer treatment strategy. Reports of Biochemistry & Molecular Biology, 10(4): 640.

Research in Cell and Tissue

Comparison of the effect of different sources of cold plasma production on the growth of MCF-7 cell line

References

References

Volume 3, Issue 1
April 2022
Pages 9-14

Comparison of the effect of different sources of cold plasma production on the growth of MCF-7 cell line

References

References

Volume 3, Issue 1April 2022Pages 9-14

Volume 3, Issue 1
April 2022
Pages 9-14