Investigating the Antimicrobial Properties of Metabolites Extracted from Cellulosimicrobium

Seratnahaei, Marjan; Eshraghi, Seyyed Saeed; Pakzad, Parviz; Zahraei-Ramazani, Alireza; Yaseri, Mehdi

doi:10.30495/rkctc.2023.74228.1052

Document Type : Original Article

Authors

¹ Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran

² Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran

³ Department of Biology and Vector Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

⁴ Department of Epidemiology and Biostatistics, Tehran University of Medical Sciences

https://doi.org/10.30495/rkctc.2023.74228.1052

Abstract

Introduction and aim: This study aimed to investigate the antimicrobial properties of metabolites extracted from cellulose microbiomes to better understand their potential as a source of new antimicrobial agents.
Methods: In this observational-analytical study, 50 soil samples were collected from different regions of Tehran province. After culturing, suspicious colonies were phenotypically and biochemically tested, and molecular identification was performed by amplifying the 16S rRNA gene. Primary screening of metabolite production with antibacterial and antifungal effects was done using the Cross-Streak Method. The antimicrobial effect of the extracted metabolite was measured using the well diffusion method and the minimum concentration of growth inhibition. Finally, the chemical composition and potential metabolite substance were determined through high-performance liquid chromatography and mass spectrometry.
Results: Five Cellulosimicrobium isolates were identified using biochemical methods and molecular identity verification. Among the five isolates suspected to be Cellulosimicrobium, strong antibacterial activity was observed in one isolate during the screening stage. Cellulosimicrobium had a strong effect on Escherichia coli and methicillin-resistant Staphylococcus aureus, but no antifungal effect was observed. The structure of the desired metabolite with the formula C₁₄H₂₄N₂O₇was identified through HPLC and Mass chromatography analysis.
Conclusion: These findings suggest that Cellulosimicrobium is a promising source for the discovery and development of new antimicrobial agents.

Keywords

Main Subjects

Bacteriology and Bacterial culture media

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Research in Cell and Tissue

Investigating the Antimicrobial Properties of Metabolites Extracted from Cellulosimicrobium

References

References

Volume 3, Issue 1
April 2022
Pages 15-22

Investigating the Antimicrobial Properties of Metabolites Extracted from Cellulosimicrobium

References

References

Volume 3, Issue 1April 2022Pages 15-22

Volume 3, Issue 1
April 2022
Pages 15-22