نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکتری دامپزشکی دانشگاه آزاد اسلامی واحد شبستر، ایران

2 گروه دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران

3 آزمایشگاه سلولی مولکولی موسسه تحقیقات واکسن و سرم سازی رازی شعبه شمالغرب، ایران

10.30495/rkctc.2023.75968.1055

چکیده

مقدمه و هدف: سلول‌های کشت‌شده طیور، بویژه سلول­های فیبروبلاست می­توانند منبع مفیدی برای محققین مختلف در زمینه مطالعات دارویی, کشت ویروس­های طیور، انسان و هم چنین تولید واکسن­های ویروسی را فراهم آورند .کشت این سلول­ها نیازمند وجود یک محیط پایه بهینه می­باشد و در حال حاضر گزینه‌های محدودی برای این محیط­های پایه وجود دارد.
روش کار: این مطالعه با هدف جداسازی سلول‌های فیبروبلاست از جوجه­های SPF، ارزیابی رشد و تکثیر این سلول­ها در سه محیط کشت DMEM با گلوگز بالا، DMEM با گلوگز پایین و محیط کشت RMI1640 که با غلظت­های مختلفی از سرم جنین گوساله (FBS) غنی شده بودند، انجام گردید.
نتایج: نتایج این مطالعه نشان ‌داد که تفاوت معنی­داری در رشد و تکثیر فیبروبلاست‌های کشت‌شده در محیط کشت DMEM با گلوگز بالای غنی شده با 10 درصد FBS در مقایسه با محیط کشت­های DMEM با گلوگز پایین و محیط کشت RMI1640 که با 10 درصد FBS غنی شده بودند، وجود داشت (P<0.05). اما تفاوت معنی­داری در نتایج زمان دو برابر شدن این سلول‌ها در سه محیط کشت، مشاهده نگردید.
نتیجه‌گیری: این نتایج نشان می­دهد که براحتی می­توان در آزمایشگاه سلول­های فیبروبلاست جنین جوجه را جداسازی نموده و با استفاده از محیط کشت­ DMEM با گلوگز بالای با 10 درصد FBS تکثیر داد. نتایج این مطالعه می­تواند در تحقیقات مختلف دارویی و ویروس­شناسی و همچنین تولید واکسن­های مختلف ویروسی مورد استفاده محققین قرار گیرد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Isolation,, propagation and optimization of SPF egg fibroblast cell culture

نویسندگان [English]

  • Mohamad Mahdi Ramezani 1
  • Ali Asghar Razmaraei Iranagh 1
  • Reaza Aghaei 2
  • Nasser Razmaraii 3

1 ، East Azerbaijan, Shabestar, Islamic Azad University

2 East Azerbaijan, Shabestar, Islamic Azad University

3 East Azarbaijan, Marand, Razi vaccine and serum research institute, northwestern branch

چکیده [English]

Introduction and aim: Cultured poultry cells can provide a valuable resource for various researchers in the field of pharmaceutical studies, the culture of poultry and human viruses, as well as the production of viral vaccines. Avian cell culture requires an optimal basic medium and currently, there are limited options for this basic medium. This means that there is still room to improve an optimal basal medium for avian cell culture.
Methods: In this study, fibroblast cells were isolated from SPF chickens and the growth and proliferation of fibroblast cells were done in three culture mediums: DMEM with high glucose, DMEM with low glucose, and RMI1640 culture medium enriched with different concentrations of fetal calf serum (FBS), were evaluated.
Results: The results of this study showed that there is a significant difference in the growth and proliferation of fibroblasts cultured in DMEM culture medium with high glucose enriched with 10% FBS compared to DMEM culture medium with low glucose and RMI1640 culture medium enriched with 10% FBS. There were (p<0.05). However, no significant difference was observed in the doubling time of these cells in three culture media.
Conclusion: These results show that it is easy to isolate chicken embryo fibroblast cells in the laboratory and provide the necessary conditions for the growth and proliferation of these cells by using a DMEM culture medium with high glucose enriched with 10% FBS. The results of this study can be used by researchers in various pharmaceutical and virological research and the production of poultry viral vaccines.

کلیدواژه‌ها [English]

  • Chick Embryo Fibroblast
  • DMEM
  • RPMI1640
  • SPF
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