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Isolation,, propagation and optimization of SPF egg fibroblast cell culture

Document Type : Original Article

Authors

1 ، East Azerbaijan, Shabestar, Islamic Azad University

2 Islamic Azad University, Shabest branch

3 East Azerbaijan, Shabestar, Islamic Azad University

4 East Azarbaijan, Marand, Razi vaccine and serum research institute, northwestern branch

10.30495/rkctc.2023.75968.1055

Abstract

Introduction and aim: Cultured poultry cells can provide a valuable resource for various researchers in the field of pharmaceutical studies, the culture of poultry and human viruses, as well as the production of viral vaccines. Avian cell culture requires an optimal basic medium and currently, there are limited options for this basic medium. This means that there is still room to improve an optimal basal medium for avian cell culture.
Methods: In this study, fibroblast cells were isolated from SPF chickens and the growth and proliferation of fibroblast cells were done in three culture mediums: DMEM with high glucose, DMEM with low glucose, and RMI1640 culture medium enriched with different concentrations of fetal calf serum (FBS), were evaluated.
Results: The results of this study showed that there is a significant difference in the growth and proliferation of fibroblasts cultured in DMEM culture medium with high glucose enriched with 10% FBS compared to DMEM culture medium with low glucose and RMI1640 culture medium enriched with 10% FBS. There were (p<0.05). However, no significant difference was observed in the doubling time of these cells in three culture media.
Conclusion: These results show that it is easy to isolate chicken embryo fibroblast cells in the laboratory and provide the necessary conditions for the growth and proliferation of these cells by using a DMEM culture medium with high glucose enriched with 10% FBS. The results of this study can be used by researchers in various pharmaceutical and virological research and the production of poultry viral vaccines.

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References
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Research in Cell and Tissue
Volume 3, Issue 2
November 2023
Pages 9-18
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