Document Type : Original Article

Authors

1 Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Cell and Molecular Biology, Ashrafi Isfahani University, Isfahan, Iran

3 Department of Biology, Kazeroon Branch, Islamic Azad University, Tehran, Iran

10.30495/rkctc.2024.76867.1057

Abstract

Background and Aim: Many studies have shown that the amniotic membrane can be used to prepare a suitable acellular sheet. At the same time, the quality of acellular sheets prepared by different methods is different. Based on this, the present study aims to obtain a suitable scaffold prepared from the amniotic membrane, investigate the characteristics and evaluate the acellular sheet derived from the amniotic membrane.
Methods: During this experimental laboratory study, the complete sample of placenta was isolated from mothers who underwent cesarean section. The amnion membrane was treated with PBS solution and 0.03% (w/v) sodium dodecyl sulfate and Tris-buffered saline (TBS) solution and decellularized.
Results: The results of examination with electron microscope showed that there is no cell content in the prepared acellular sheet.
Conclusion: Overall, the results of this research showed that the acellular sheet prepared from human amniotic membrane has good quality as a biological scaffold.

Keywords

Main Subjects

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