Original Article
tissue engineering and Regenerative medicine
Akram Fadavi; Rahim Ahmadi; Sepideh Shahbaz Gahrouei; Maryam Hassan Nasab
Abstract
Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast ...
Read More
Background and aim: Many studies have shown that chitosan scaffolds can be used to load stem cells, but the findings are still challenging. Therefore, the present study evaluated the properties of chitosan scaffolds synthesized by the enzymatic method and their efficiency for loading neonatal fibroblast cells.Methods: During this experimental-laboratory study, the human neonatal foreskin was prepared and the fibroblast cells of the dermal layer were isolated and cultured. Cell viability was determined by flow cytometry and cell identity was determined using a Vimentin marker. After the preparation of chitosan hydrogel, fibroblast cells were loaded on it and the cytotoxic effect of hydrogel on cells was evaluated using the MTT assay. Data were analyzed using a t-test.Results: Cell counts showed that each dense T75 flask contained about 2 million cells. Isolated cells expressed the Vimentin marker at a high level. Fibroblasts were observed with good adhesion and uniform distribution on chitosan hydrogel. According to the results of the MTT test, the prepared hydrogel had no significant toxicity effect on fibroblast cells.Conclusion: The results of this study showed that chitosan scaffolding can be a suitable scaffold for loading fibroblast cells and transporting these cells for transplantation to damaged tissues.
Original Article
tissue engineering and Regenerative medicine
Elaheh Roshanzadeh; Abbas Sahebghadam Lotfi; Sareh Arjmand
Volume 2, Issue 2 , October 2021, Pages 8-20
Abstract
Introduction and Aim: Using 3D culture to increase cell density and creating a suitable bedrock for the growth and proliferation and differentiation of stem cells is one of the important methods for their application in tissue engineering. In this study, mesenchymal stem cells of adipose tissue because ...
Read More
Introduction and Aim: Using 3D culture to increase cell density and creating a suitable bedrock for the growth and proliferation and differentiation of stem cells is one of the important methods for their application in tissue engineering. In this study, mesenchymal stem cells of adipose tissue because of ease of access and abundance have been used to differentiate into hepatocyte-like cells to investigate the effect of 3D cultures on gelatin/laminin 3D scaffolds.Methods: Mesenchymal stem cells were extracted from subcutaneous fat of the abdominal region of C57 mice by enzymatic digestion using collagenase enzyme. The extracted stem cells were confirmed by flow cytometry analysis and observation of the expression of specific stem cells markers such as CD105, CD44, and the absence of specific leukocyte (CD34) and hematopoietic (CD45). The non-toxicity of the scaffold was also investigated by the MTT method. Stem cells were cultured on a polystyrene surface and laminin and gelatin/laminin scaffold and treated with growth factors for 21 days in two stages. Cell differentiation was investigated by biochemical methods including examination of secretion indices such as urea and glycogen storage.Results: The expression of specific stem cells markers such as CD105, CD44, and the absence of CD34 and CD45 were proved. The ability to differentiate these cells into adipocytes and osteocytes has been proven.Conclusion: The results show the presence of a three-dimensional gelatin/laminin scaffold increases the adhesion, proliferation, and differentiation of fatty mesenchymal cells to hepatocyte-like cells.
Original Article
Cell culture and Tissue culture
Arefeh Kamali; Abbas Zare MirakAbadi
Volume 2, Issue 2 , October 2021, Pages 21-27
Abstract
Introduction and Aim: Many colubrid snakes produce toxic oral secretions and some species have caused severe reactions in humans. In this research toxicity of Duvernoy´s gland secretion (DGS) from Hemorrhois ravergieri (aglyph) has been studied.Methods: In this study, the effect of Hemorrhois ravergieri ...
Read More
Introduction and Aim: Many colubrid snakes produce toxic oral secretions and some species have caused severe reactions in humans. In this research toxicity of Duvernoy´s gland secretion (DGS) from Hemorrhois ravergieri (aglyph) has been studied.Methods: In this study, the effect of Hemorrhois ravergieri saliva on human dermal fibroblasts (HDF) cells growth was determined by the inverted microscope and MTT assay. The integrity of the cell membrane through LDH release was evaluated as well.Results: The MTT assay and Neutral red assay showed a significant (p˂0.05) cytotoxic effect of Hemorrhois ravergieri salvia on HDF cells growth after 24 h treatment. Additionally, Hemorrhois ravergieri venom caused a significant increase in LDH release (p˂0.05). Numerous morphological abnormalities were observed in cells exposed to the DGS and showed loss of their common polygonal shape, appearing as several roughly rounded cells of variable size. Conclusion: The Hemorrhois ravergieri saliva causes cytotoxic effects on HDF cells by the necrotic mechanism. This colubrid snake bite causes local symptoms, so this research suggests a more careful evaluation of the victims when considering the medical treatment to be adopted.
Original Article
Cancer tissue and cell culture
Mina Maftoon; Rahim Ahmadi
Volume 2, Issue 2 , October 2021, Pages 28-34
Abstract
Introduction and Aim: Fibroblastoma is a common skin malignancy and a common cause of morbidity worldwide. Studies have shown that sex steroid hormones including progesterone have cytotoxic effects on cancer cells. According to this, the present study aims to determine the effects of progesterone cytotoxic ...
Read More
Introduction and Aim: Fibroblastoma is a common skin malignancy and a common cause of morbidity worldwide. Studies have shown that sex steroid hormones including progesterone have cytotoxic effects on cancer cells. According to this, the present study aims to determine the effects of progesterone cytotoxic concentration on iNOs expression in fibroblastoma (L929) cells.Methods: Cell viability was measured using MTT assay in cells exposed to 0.001, 0.01, 0.1, 1, and 10 mg/ml of progesterone, and IC50 dose was determined. NO concentration levels were also measured using the Griess test. The expression level of iNOS was measured by real-time RT-PCR. Data were analyzed using SPSS by one-way ANOVA.Results: Viability significantly decreased in fibroblastoma cells exposed to 1 and 10 mg/ml of progesterone (P<0.001). The relative expression level of iNOS significantly increased in cells exposed to IC50 dose of progesterone (P<0.001). The relative concentration of NO significantly increased in fibroblastoma cells exposed to 0.01, 0.1, and 1 mg/ml of progesterone (P<0.001, P<0.001, and P<0.05, respectively).Conclusion: Progesterone has cytotoxic effects on fibroblastoma cancer cells due partly to the effects of the hormone on iNOS expression level and increased NO that probably induces apoptosis in fibroblastoma cancer cells.
Original Article
Cell culture and Tissue culture
Sahar Salmanzadeh Zehkesh; Naser Mohammadpour Donighi
Volume 2, Issue 2 , October 2021, Pages 35-42
Abstract
Introduction and Aim: Alginate nanoparticles have been used in novel drug delivery systems due to marvelous features including biocompatibility, biodegradable, mucoadhesive, and non-toxic. At present various types of adjuvants are used against snake venom, as a solution, suspension, or emulsion systems, ...
Read More
Introduction and Aim: Alginate nanoparticles have been used in novel drug delivery systems due to marvelous features including biocompatibility, biodegradable, mucoadhesive, and non-toxic. At present various types of adjuvants are used against snake venom, as a solution, suspension, or emulsion systems, which are used in different centers. The purpose of this study is to design a novel type of antigen delivery system as an adjuvant from alginate nanoparticles.Methods: Alginate nanoparticles were prepared based on the ionic gelation process. The nanoparticles' particle size, morphology, zeta potential, in vitro release behavior, and structure of nanoparticles contained venom were analyzed by SEM, DLS, and FT-IR spectroscopy. Cell viability of SW-480 cells was measured using an MTT assay.Results: The particles were spherical. The optimum particles size of alginate and venom-loaded nanoparticles were 118 and 169 nm, respectively. Viability significantly decreased in cells exposed to upper 20 µg/ml o (P<0.05).Conclusion: Our results suggested that alginate nanoparticles, which were prepared in our study possibly, could be used as an alternative for traditional adjuvant systems.
Original Article
Animal cell and culture
Mohammad Karamali Gilaneh; Rahim Ahmadi; Fatemeh Amini Khodashahri
Volume 2, Issue 2 , October 2021, Pages 43-50
Abstract
Introduction: Many studies have shown the relationship between ultrasound and the function of the reproductive system. However, the mechanism of ultrasound waves action on sex hormones and testicular tissue is not perfectly clear. Accordingly, the present study investigated the effects of ultrasound ...
Read More
Introduction: Many studies have shown the relationship between ultrasound and the function of the reproductive system. However, the mechanism of ultrasound waves action on sex hormones and testicular tissue is not perfectly clear. Accordingly, the present study investigated the effects of ultrasound waves on serum levels of DHEA-SO4, testosterone and testicular tissue in male rats.Materials and Method: Male wistar rats were divided into three groups: control group which was not exposed to ultrasound waves, rats that were exposed to ultrasound waves for 1h/day, and rats that were exposed to ultrasound waves for 6h/day. The serum levels of testosterone and DHEA-SO4 were measured using ELISA. The testicular tissue slides were prepared and analyzed microscopically.Data were analyzed using one-way ANOVA. Results: Serum levels of testosterone and DHEA-SO4 and spermatogonia, spermatocytes, sertoli cell count and seminiferous tubules morphology did not significantly changed in groups exposed to ultrasound waves compared to control animals. Conclusion: Our findings show that ultrasound waves do not significantly influence male reproductive system function and histology.
Editorial
Volume 2, Issue 2 , October 2021
