Original Article
Cell culture and Tissue culture
Shahin Seyf; Rahim Ahmadi; Sara Najafi
Abstract
Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in ...
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Background and aim: Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in human red blood cells.Methods: In this experimental laboratory study, blood samples were obtained from healthy individuals, and blood samples were divided into the control group (treated with normal saline) and 2, 4, and 6 mg/kg of sulfasalazine receiving groups. Samples treated with 2 mg/kg of sulfasalazine were treated with 2, 4, and 6 mg/kg of Pissum sativum L and Aloe vera extract, and the erythrocyte membrane stability was calculated by standard methods. Data were analyzed using a one-way analysis of variance.Results: Sulfasalazine significantly reduced the stability of the erythrocyte membrane compared to the control group. Treatment of samples with concentrations of 4 and 6 mg/kg Pissum sativum L extract significantly increased membrane stability compared to the groups treated with sulfasalazine. Treatment with 2 and 4 mg/kg of Aloe vera extract did not increase membrane stability and a 6 mg/kg of Aloe vera extract non-significantly increased the membrane stability.Conclusion: Unlike Aloe vera, Pissum sativum L extract can increase the membrane stability of red blood cells in healthy people.
Original Article
Microbiology and Microbial media
Niloofar Seyfollahi; Simin Nabizadeh; Zahra Shafiei
Abstract
Introduction and Aim: In the second half of the last century, Pseudomonas aeruginosa is considered an important hospital pathogen, according to the US Centers for Disease Control. Pseudomonas aeruginosa is the fifth pathogen among hospital microorganisms, which comprises 10% of hospital-acquired infections, ...
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Introduction and Aim: In the second half of the last century, Pseudomonas aeruginosa is considered an important hospital pathogen, according to the US Centers for Disease Control. Pseudomonas aeruginosa is the fifth pathogen among hospital microorganisms, which comprises 10% of hospital-acquired infections, as the purslane plant has a variety of vitamins and minerals in fatty acids and as a fever antiseptic medication and it is used against spasm.Material and method: To evaluate the antimicrobial activity of aqueous and ethanolic extracts of Purslane, four standard microbial methods including mixed agar diffusion method, disk diffusion, the minimum inhibitory concentration of MIC, and minimum killer concentration of MBC using the microdilution method.Results: The results showed that silver nanoparticles obtained from purslane significantly reduced the growth of Pseudomonas, however, treatments of this bacterium with only purslane extract did not cause any effects on the growth of Pseudomonas.Conclusion: It can be said that the effect of silver nanoparticles from purslane was significantly higher than the extracts extracted from purslane. pH 10 combined with 2 mM nitrate salt yielded the most suitable dimensions for silver nanoparticles, and silver nanoparticles synthesized from purslane had far greater antibacterial effect than the extract extracted from purslane. So, this nanoparticle could be a good candidate to fight infection caused by Pseudomonas aeruginosa.
Original Article
Cell culture and Tissue culture
sasan Kalantari; Tahereh Naji; Rahim Ahmadi
Abstract
Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar ...
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Introduction and Aim: One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The present study aimed to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar rats.Methods: In this study, 20 two-month-old adult male Wistar albino rats weighing approximately 200-150 g were studied in two groups: the control group (healthy) and the busulfan group. After this period, the testicular tissue of the mice was isolated and evaluated for the expression of Nrf-2 and Keap-1 genes, as well as changes in testosterone, FSH, and LH. Data were analyzed using SPSS software and a one-way ANOVA statistical test.Results: The statistical analysis of the results showed that the expression of Nrf-2 and Keap-1 genes in the treatment group (busulfan) decreased compared to the control group, but this decrease was not statistically significant (0.2951 and P = 0.3528). LH and testosterone levels were not significantly different between the control and treated groups (0.1327 and P = 0.0809) but FSH levels were significant between the two groups (P = 0.0189).Conclusion: The results of this study showed that busulfan can affect the level of FSH, which is effective in spermatogenesis, by causing changes in the expression of keap1 and Nrf-2 genes and causing infertility. Therefore, these genetic and hormonal factors can be used to predict infertility.
Original Article
Cancer tissue and cell culture
Mahdi Azadmanesh; Tahereh Naji; Abdolreza Mohammadnia
Abstract
Introduction and aim: Genes play a very important role in the development of cancer. This study aimed to evaluate three very important genes (C-fos, C-jun, and P53) in Wistar mice's stomach tissue cancer cells designed by dimethylhydrazine.Methods: In this study, forty male Wistar mice, 57 weeks old, ...
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Introduction and aim: Genes play a very important role in the development of cancer. This study aimed to evaluate three very important genes (C-fos, C-jun, and P53) in Wistar mice's stomach tissue cancer cells designed by dimethylhydrazine.Methods: In this study, forty male Wistar mice, 57 weeks old, were evaluated and divided into four groups of Ten. In the control group, the second group received Cinnamaldehyde-free carcinogens, the third group was healthy with the Cinnamaldehyde, and the fourth group received carcinogens with the Cinnamaldehyde at the same time. Then, the gastric tissue of mice was isolated and evaluated for the expression of C-fos, C-Jun, and P53 genes. The data were analyzed with One-way ANOVA using SPSS.Results: Statistical analysis of the results showed that the expression of C-fos, C-Jun, and P53 genes in the carcinogen receptor with the Cinnamaldehyde was increased compared to the control group. This increase was statistically significant for two genes C-fos (P = 0.0146) and P53 (P = 0.0212) and for the C-Jun gene (P = 0.01604) this increase was not significant.Conclusion: The results of this study showed that Cinnamaldehyde can alter the expression of important genes C-fos, C-Jun, and P53 that are effective in causing gastric cancer.
Original Article
Animal cell and culture
Sajjad Gharakhani Deh Sorkhi; Marzyieh Taqipour Dehkordi
Abstract
Introduction and aim: Hydatid cyst surgery is the most effective treatment. Hydatid cyst recurrence after surgery and the lack of effective and safe Askvlysydl is one of the most important research topics in the treatment of hydatid cysts, which have negative effects on human organs. This study aimed ...
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Introduction and aim: Hydatid cyst surgery is the most effective treatment. Hydatid cyst recurrence after surgery and the lack of effective and safe Askvlysydl is one of the most important research topics in the treatment of hydatid cysts, which have negative effects on human organs. This study aimed to conduct a full investigation on the impact of the different stages of the parasite herbs and ingredients, to treat helminth infections will significantly contribute to a significant reduction in helminth infections.Methods: In the present study, the liver of sheep infected with hydatid cyst collected from the slaughterhouse was isolated and the fluid of cysts containing live protoscolices was aspirated under sterile conditions.Results: After examining the viability protoscolex 1% eosin staining, protoscolex activities Plumbing chamomile extract diluted concentrations of 5%, 2.50%, 1.25%, 0.66%, 0.33% and in time of 0, 10, 20, 30, 40, 50, 60, 120, h24, h72 were studied. 0/5 chamomile extract concentration in the first two hours protoscolex of hydatid cyst can be destroyed. The protoscolices lowest percentage was in the concentration of 0.33% percent in an hour. Dilution of engineering activity increased protoscolex Decreases.Conclusion: In addition to being affordable chamomile extract, in a short time leads to protoscolex within the hydatid cyst. Scolexes property in dilution laying downtime is less effective.
Original Article
Plant Cell Culture and Tissue
Leila Zarandi-Miandoab; Zahra Oraghi Ardebili
Abstract
To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases ...
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To gain insight into metal-based nanomaterials, this study figured out the physiological and molecular behaviors of Melissa officinalis to supplementation of nutrient solution with red nano elemental selenium (nSe; 0, 10, and 50 mgl-1) or bulk Se (BSe). The nSe10 application led to drastic increases in root and shoot fresh weights, and chlorophyll content. While, the nSe at 50 mgl-1 exhibited severe phyotoxicity. Also, nSe10 enhanced uptake and accumulation of Ca and Mg in both leaf and root, contrasted to the nSe50-treated plants. The applied supplements modified phenylalanine ammonia lyase activity, concentrations of flavonids, glutathione, and proline. Moreover, these supplements in the dose and type-dependent manners changed the activities of catalase. Furthermore, the applied treatments up-regulated the expression of phenylalanine ammonia-lyase (PAL) and Coumarate: CoA-ligase (4CL) genes. The comparative physiological and molecular evidence on phytotoxicity and potential advantages of nSe and its bulk counterpart was provided as a theoretical basis for exploiting in food, agricultural, and pharmaceutical industries.
Editorial
Volume 2, Issue 3 , December 2021
